Q8NPZ2 · PIPS_CORGL
- ProteinPhosphatidylinositol phosphate synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids219 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.
Catalytic activity
- 1D-myo-inositol 3-phosphate + a CDP-1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H+
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H+
Cofactor
Note: Contains a di-nuclear catalytic Mg2+ center.
Pathway
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 29-32 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: NQLT | ||||||
Binding site | 66 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 66 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 69 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 70 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 74 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 80 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 87 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 87 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 91 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 91 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphotransferase activity, for other substituted phosphate groups | |
Biological Process | phospholipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphatidylinositol phosphate synthase
- EC number
- Short namesPIP synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Corynebacteriaceae > Corynebacterium
Accessions
- Primary accessionQ8NPZ2
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 31-47 | Helical | ||||
Sequence: LTLVSAGLTVGVALLLI | ||||||
Transmembrane | 53-72 | Helical | ||||
Sequence: IWAAVLTGLFAAFDMIDGTV | ||||||
Transmembrane | 93-110 | Helical | ||||
Sequence: ITDGALFGAITWWLVYSY | ||||||
Transmembrane | 116-133 | Helical | ||||
Sequence: LVAASLVCLVASQVISYV | ||||||
Transmembrane | 154-171 | Helical | ||||
Sequence: RLIVSLVGLGLTGMGVPY | ||||||
Transmembrane | 177-194 | Helical | ||||
Sequence: LWALAAGSIYTVVQRLVM |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000448364 | 1-219 | Phosphatidylinositol phosphate synthase | |||
Sequence: MLGLHGRKPAQVIVEPVAKLMIKLKVTPNQLTLVSAGLTVGVALLLIPTGHLIWAAVLTGLFAAFDMIDGTVARMQGGGTKFGATLDATCDRITDGALFGAITWWLVYSYDAPQALVAASLVCLVASQVISYVKARGEASGFTMDGGLVERPERLIVSLVGLGLTGMGVPYAIDVALWALAAGSIYTVVQRLVMAGKSPLAKEFTKAPAGAKADYSNTK |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length219
- Mass (Da)22,834
- Last updated2002-10-01 v1
- ChecksumCFFB170796DF4DE5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000036 EMBL· GenBank· DDBJ | BAB99062.1 EMBL· GenBank· DDBJ | Genomic DNA |