Q8NNP0 · SYI_CORGL

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site630ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • Ordered locus names
      Cgl2148, cg2359

Organism names

Accessions

  • Primary accession
    Q8NNP0
  • Secondary accessions
    • Q6M3S6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000985381-1054Isoleucine--tRNA ligase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif58-68'HIGH' region
Region522-543Disordered
Motif627-631'KMSKS' region

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,054
  • Mass (Da)
    117,404
  • Last updated
    2002-10-01 v1
  • Checksum
    C3333C86C8554B1F
MSEAVGGVYPQVDLSGGSSRFPEMEENVLSYWKKDDTFQASIDQRDGAEDYVFYDGPPFANGLPHYGHLLTGYVKDIVPRYQTMRGYRVPRVFGWDTHGLPAELEAEKQLGIKDKGEIEAMGLAKFNEYCATSVLQYTKEWEEYVTRQARWVDFENGYKTMDLSFMESVIWAFKELYDKGLIYQGFRVLPYSWAEHTPLSNQETRLDDSYKLRQDPTLTVTFPVTGVVEGSSANAGLVGALALAWTTTPWTLPSNLALAVNPAVTYALVEVAEDGEAEFVGKRVLLAKDLVGSYAKELGAEAVIVSEHPGSELVGLTYEPIFGYFRDHANGFQILGAEYVTTEDGTGIVHQAPAFGEDDMNTCNAAGIEPVIPVDIDGKFTGLVPEYQGQLVFDANKDIIKDLKAAGRVVRHQTIEHSYPHSWRSGEPLIYMALPSWFVNVTEIRDRMVEVNQDIEWMPAHIRDGQFGKWLEGARDWNISRSRYWGSPIPAWVSDNDEYPRVDVYGSLDELEADFGVRPKSLHRPDIDELTRPNPDDPTGKSTMRRVTDVLDVWFDSGSMPFAQVHYPFENKEWFDTHAPADFIVEYIGQTRGWFYLLHVLSTALFDRPAFKKVVAHGIVLGDDGLKMSKSKGNYPNVNEVFDRDGSDAMRWFLMSSPILRGGNLIVTEKGIREGVRQAQLPMWNAYSFLQLYTSKNATWSVDSTDVLDRYILAKLHDLVAETQAALDGTDIAKACDLVRNFCDALTNWYVRRSRDRFWAGDEAHPEAFNTLYTVLETLTRVAAPLLPMTTEVIWRGLTGERSVHLTDFPSAESFPADADLVRTMDEIRGVCSAASSVRKAHKLRNRLPLPGLTVALPDSARLADFASIIRDEVNVKNVDLTSDVDSVGTFEVVVNAKVAGPRLGKDVQRVIKAVKAGNYTREGDVVVADGIELNEGEFTERLVAANPDSTAQIDGVDGLVVLDMEVTEELEAEGWAADAIRGLQDARKNSGFEVSDRISVVVSVPEDKKEWITTHADHIAAEVLATSFEIVTDALDGETHDIVAGVTAKVTKN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000036
EMBL· GenBank· DDBJ
BAB99541.1
EMBL· GenBank· DDBJ
Genomic DNA
BX927154
EMBL· GenBank· DDBJ
CAF20488.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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