Q8NC96 · NECP1_HUMAN
- ProteinAdaptin ear-binding coat-associated protein 1
- GeneNECAP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids275 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in endocytosis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | clathrin vesicle coat | |
Cellular Component | clathrin-coated pit | |
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Biological Process | endocytosis | |
Biological Process | protein transport | |
Biological Process | vesicle-mediated transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdaptin ear-binding coat-associated protein 1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8NC96
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with AP-2 at the plasma membrane.
Keywords
- Cellular component
Disease & Variants
Involvement in disease
Developmental and epileptic encephalopathy 21 (DEE21)
- Note
- DescriptionA severe disease characterized by intractable seizures, profound global developmental delay, and persistent severe axial hypotonia as well as appendicular hypertonia.
- See alsoMIM:615833
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_034153 | 224 | in dbSNP:rs2231752 | |||
Sequence: D → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 350 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000213067 | 1-275 | UniProt | Adaptin ear-binding coat-associated protein 1 | |||
Sequence: MATELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKTAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLCIGNITNKKGGASKPRTARGGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGSDADILLDLDSPAPVTTPAPTPVSVSNDLWGDFSTASSSVPNQAPQPSNWVQF | |||||||
Modified residue (large scale data) | 140 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 180 | UniProt | Omega-N-methylarginine | ||||
Sequence: R | |||||||
Modified residue (large scale data) | 207 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 211 | UniProt | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 211 | PRIDE | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8NC96 | AP1B1 Q10567-3 | 3 | EBI-2609792, EBI-11978055 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 170-191 | Disordered | ||||
Sequence: KGGASKPRTARGGGLSLLPPPP | ||||||
Motif | 252-255 | WXXF motif 1 | ||||
Sequence: WGDF | ||||||
Region | 254-275 | Disordered | ||||
Sequence: DFSTASSSVPNQAPQPSNWVQF | ||||||
Motif | 272-275 | WXXF motif 2 | ||||
Sequence: WVQF |
Domain
The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2 (By similarity).
Sequence similarities
Belongs to the NECAP family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8NC96-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length275
- Mass (Da)29,737
- Last updated2005-03-29 v2
- Checksum22FC4CCEC7E3B713
Q8NC96-2
- Name2
- NoteMay be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Computationally mapped potential isoform sequences
There are 11 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1W2PPF5 | A0A1W2PPF5_HUMAN | NECAP1 | 110 | ||
A0A1W2PNT6 | A0A1W2PNT6_HUMAN | NECAP1 | 179 | ||
A0A1W2PR27 | A0A1W2PR27_HUMAN | NECAP1 | 110 | ||
A0A1W2PQK9 | A0A1W2PQK9_HUMAN | NECAP1 | 23 | ||
A0A1W2PR09 | A0A1W2PR09_HUMAN | NECAP1 | 271 | ||
A0A1W2PRW5 | A0A1W2PRW5_HUMAN | NECAP1 | 93 | ||
A0A1W2PRL5 | A0A1W2PRL5_HUMAN | NECAP1 | 254 | ||
A0A1W2PRM0 | A0A1W2PRM0_HUMAN | NECAP1 | 250 | ||
A0A1W2PRQ7 | A0A1W2PRQ7_HUMAN | NECAP1 | 121 | ||
F5GYH1 | F5GYH1_HUMAN | NECAP1 | 240 | ||
F5H2U7 | F5H2U7_HUMAN | NECAP1 | 116 |
Sequence caution
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 77 | in Ref. 2; CAB43373 | ||||
Sequence: Y → F | ||||||
Alternative sequence | VSP_013232 | 102 | in isoform 2 | |||
Sequence: R → G | ||||||
Alternative sequence | VSP_013233 | 103-275 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 232 | in Ref. 1; BAC11250 | ||||
Sequence: D → G | ||||||
Sequence conflict | 239 | in Ref. 1; BAC11264 | ||||
Sequence: T → A |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK074858 EMBL· GenBank· DDBJ | BAC11250.1 EMBL· GenBank· DDBJ | mRNA | ||
AK074880 EMBL· GenBank· DDBJ | BAC11264.1 EMBL· GenBank· DDBJ | mRNA | ||
AK074923 EMBL· GenBank· DDBJ | BAC11296.1 EMBL· GenBank· DDBJ | mRNA | ||
AK075013 EMBL· GenBank· DDBJ | BAC11352.1 EMBL· GenBank· DDBJ | mRNA | ||
AL050272 EMBL· GenBank· DDBJ | CAB43373.2 EMBL· GenBank· DDBJ | mRNA | ||
BC002888 EMBL· GenBank· DDBJ | AAH02888.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BC067367 EMBL· GenBank· DDBJ | AAH67367.1 EMBL· GenBank· DDBJ | mRNA | ||
BC084551 EMBL· GenBank· DDBJ | AAH84551.1 EMBL· GenBank· DDBJ | mRNA | ||
BC110876 EMBL· GenBank· DDBJ | AAI10877.1 EMBL· GenBank· DDBJ | mRNA |