Q8NBN7 · RDH13_HUMAN
- ProteinRetinol dehydrogenase 13
- GeneRDH13
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids331 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Retinol dehydrogenase with a clear preference for NADP. Oxidizes all-trans-retinol, but seems to reduce all-trans-retinal with much higher efficiency (PubMed:18039331).
Has no activity toward steroids (PubMed:18039331).
Has no activity toward steroids (PubMed:18039331).
Catalytic activity
- all-trans-retinol + NADP+ = all-trans-retinal + H+ + NADPH
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.2 μM | all-trans-retinal | |||||
3 μM | all-trans-retinol | |||||
1.5 μM | NADPH | |||||
6000 μM | NAD |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
230 nmol/min/mg | with all-trans-retinal as substrate | ||||
5 nmol/min/mg | with all-trans-retinol as substrate | ||||
230 nmol/min/mg | with NADPH as substrate |
Pathway
Cofactor metabolism; retinol metabolism.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | all-trans-retinol dehydrogenase (NADP+) activity | |
Biological Process | eye photoreceptor cell development | |
Biological Process | response to high light intensity | |
Biological Process | retina layer formation | |
Biological Process | retinal metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameRetinol dehydrogenase 13
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8NBN7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Peripheral membrane protein
Note: Localized on the outer side of the inner mitochondrial membrane.
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 860 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000054768 | 2-331 | Retinol dehydrogenase 13 | |||
Sequence: SRYLLPLSALGTVAGAAVLLKDYVTGGACPSKATIPGKTVIVTGANTGIGKQTALELARRGGNIILACRDMEKCEAAAKDIRGETLNHHVNARHLDLASLKSIREFAAKIIEEEERVDILINNAGVMRCPHWTTEDGFEMQFGVNHLGHFLLTNLLLDKLKASAPSRIINLSSLAHVAGHIDFDDLNWQTRKYNTKAAYCQSKLAIVLFTKELSRRLQGSGVTVNALHPGVARTELGRHTGIHGSTFSSTTLGPIFWLLVKSPELAAQPSTYLAVAEELADVSGKYFDGLKQKAPAPEAEDEEVARRLWAESARLVGLEAPSVREQPLPR | ||||||
Modified residue | 323 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8NBN7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length331
- Mass (Da)35,932
- Last updated2005-03-29 v2
- ChecksumDC5C1A6E54F1E6CC
Q8NBN7-2
- Name2
- Differences from canonical
- 1-71: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_040383 | 1-71 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 106 | in Ref. 2; BAC11591 | ||||
Sequence: E → V |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY358473 EMBL· GenBank· DDBJ | AAQ88837.1 EMBL· GenBank· DDBJ | mRNA | ||
AK075392 EMBL· GenBank· DDBJ | BAC11591.1 EMBL· GenBank· DDBJ | mRNA | ||
AC011476 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC009881 EMBL· GenBank· DDBJ | AAH09881.1 EMBL· GenBank· DDBJ | mRNA |