Q8N8Q3 · ENDOV_HUMAN
- ProteinEndonuclease V
- GeneENDOV
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids282 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Isoform 1
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:23912683, PubMed:23912718, PubMed:25195743, PubMed:27573237, PubMed:31703097).
Active against both single-stranded and double-stranded RNAs (PubMed:25195743, PubMed:31703097).
Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed:23912718).
Cleaves mRNAs and tRNAs containing inosine (PubMed:23912683, PubMed:31703097).
Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed:23912718, PubMed:27573237).
Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed:23139746).
Active against both single-stranded and double-stranded RNAs (PubMed:25195743, PubMed:31703097).
Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed:23912718).
Cleaves mRNAs and tRNAs containing inosine (PubMed:23912683, PubMed:31703097).
Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed:23912718, PubMed:27573237).
Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed:23139746).
Isoform 6
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097).
Active against both single-stranded and double-stranded RNAs (PubMed:31703097).
Cleaves tRNAs containing inosine (PubMed:31703097).
Active against both single-stranded and double-stranded RNAs (PubMed:31703097).
Cleaves tRNAs containing inosine (PubMed:31703097).
Isoform 7
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097).
Active against both single-stranded and double-stranded RNAs (PubMed:31703097).
Cleaves tRNAs containing inosine (PubMed:31703097).
Active against both single-stranded and double-stranded RNAs (PubMed:31703097).
Cleaves tRNAs containing inosine (PubMed:31703097).
Cofactor
Activity regulation
Isoform 1
Inhibited by normal intracellular concentrations of ATP.
Features
Showing features for binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic stress granule | |
Cellular Component | nucleolus | |
Molecular Function | DNA binding | |
Molecular Function | endodeoxyribonuclease activity, producing 5'-phosphomonoesters | |
Molecular Function | magnesium ion binding | |
Molecular Function | RNA endonuclease activity, producing 5'-phosphomonoesters | |
Molecular Function | single-stranded RNA binding | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndonuclease V
- EC number
- Short nameshEndoV
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8N8Q3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform 1
Note: Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with PABPC1.
Isoform 6
Note: Relocalizes to cytoplasmic stress granules upon cellular stress.
Isoform 7
Note: Relocalizes to cytoplasmic stress granules upon cellular stress.
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_046285 | 29 | in dbSNP:rs35549084 | |||
Sequence: V → I | ||||||
Mutagenesis | 35-40 | Does not gain activity against single- or double-stranded DNA. | ||||
Sequence: Missing | ||||||
Mutagenesis | 52 | Abolishes ribonuclease activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 57-60 | Does not gain activity against single- or double-stranded DNA. | ||||
Sequence: KGDS → QGGE | ||||||
Mutagenesis | 90-93 | Abolishes ability to bind branched DNA and RNA. | ||||
Sequence: PYVS → GGGG | ||||||
Mutagenesis | 91 | Abolishes ribonuclease activity without affecting ability to bind branched DNA. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 100 | Abolishes ribonuclease activity. | ||||
Sequence: E → A | ||||||
Natural variant | VAR_046286 | 112 | in dbSNP:rs34933300 | |||
Sequence: R → Q | ||||||
Natural variant | VAR_046287 | 114 | in dbSNP:rs41298706 | |||
Sequence: K → R | ||||||
Mutagenesis | 115-119 | Does not gain activity against single- or double-stranded DNA. | ||||
Sequence: Missing | ||||||
Natural variant | VAR_046288 | 141 | in dbSNP:rs41299812 | |||
Sequence: H → Y | ||||||
Mutagenesis | 161-166 | Does not gain activity against single- or double-stranded DNA. | ||||
Sequence: Missing | ||||||
Mutagenesis | 171 | No effect on subcellular location or activity; when associated with A-174. | ||||
Sequence: E → A | ||||||
Mutagenesis | 174 | No effect on subcellular location or activity; when associated with A-171. | ||||
Sequence: R → A | ||||||
Natural variant | VAR_046289 | 201 | in dbSNP:rs35929621 | |||
Sequence: D → N | ||||||
Mutagenesis | 225 | No significant effect on activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 226 | No significant effect on activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 227 | 68% decrease in activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 228 | 46% decrease in activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 248-249 | Abolishes ability to bind branched DNA and RNA. | ||||
Sequence: RK → AA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 423 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000349223 | 1-282 | UniProt | Endonuclease V | |||
Sequence: MALEAAGGPPEETLSLWKREQARLKAHVVDRDTEAWQRDPAFSGLQRVGGVDVSFVKGDSVRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKEPGLMPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHKEKIRLLQTRGDSFPLLGDSGTVLGMALRSHDRSTRPLYISVGHRMSLEAAVRLTCCCCRFRIPEPVRQADICSREHIRKSLGLPGPPTPRSPKAQRPVACPKGDSGESSALC | |||||||
Modified residue (large scale data) | 203 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 258 | PRIDE | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 261 | PRIDE | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Expression
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 250-282 | Disordered | ||||
Sequence: SLGLPGPPTPRSPKAQRPVACPKGDSGESSALC |
Sequence similarities
Belongs to the endonuclease V family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 7 isoforms produced by Alternative splicing.
Q8N8Q3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymshENDOV 282
- Length282
- Mass (Da)30,792
- Last updated2002-10-01 v1
- ChecksumEDB10210FB508A51
Q8N8Q3-2
- Name2
- Differences from canonical
- 77-121: Missing
Q8N8Q3-3
- Name3
Q8N8Q3-4
- Name4
Q8N8Q3-5
- Name5
- Differences from canonical
- 1-194: Missing
Q8N8Q3-6
- Name6
- SynonymshENDOV 308
- Differences from canonical
- 280-282: ALC → GGAPSPQRQADRTTPGGRRSTAQHQVGQR
Q8N8Q3-7
- Name7
- SynonymshENDOV 309
- Differences from canonical
- 280-282: ALC → GEGQPPQDHSPGPRTAPRPGSQEQAGKDWQ
Computationally mapped potential isoform sequences
There are 14 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H0YBX8 | H0YBX8_HUMAN | ENDOV | 131 | ||
H0YBZ5 | H0YBZ5_HUMAN | ENDOV | 170 | ||
H0YAY5 | H0YAY5_HUMAN | ENDOV | 236 | ||
I3L4V7 | I3L4V7_HUMAN | ENDOV | 155 | ||
I3L4M6 | I3L4M6_HUMAN | ENDOV | 109 | ||
I3L485 | I3L485_HUMAN | ENDOV | 105 | ||
I3L1V3 | I3L1V3_HUMAN | ENDOV | 97 | ||
I3L0L4 | I3L0L4_HUMAN | ENDOV | 115 | ||
E5RHX4 | E5RHX4_HUMAN | ENDOV | 50 | ||
E5RII5 | E5RII5_HUMAN | ENDOV | 138 | ||
E5RJ92 | E5RJ92_HUMAN | ENDOV | 97 | ||
E5RFW0 | E5RFW0_HUMAN | ENDOV | 105 | ||
A0A0G2JLD4 | A0A0G2JLD4_HUMAN | ENDOV | 8 | ||
E5RGZ9 | E5RGZ9_HUMAN | ENDOV | 123 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_035228 | 1-194 | in isoform 4 and isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 19 | in Ref. 1; BAC03912 | ||||
Sequence: R → W | ||||||
Alternative sequence | VSP_035229 | 77-121 | in isoform 2 and isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_035230 | 239-282 | in isoform 4 | |||
Sequence: ADICSREHIRKSLGLPGPPTPRSPKAQRPVACPKGDSGESSALC → HFVERGGESTRPRLIPDRTRW | ||||||
Alternative sequence | VSP_035231 | 280-282 | in isoform 3 and isoform 7 | |||
Sequence: ALC → GEGQPPQDHSPGPRTAPRPGSQEQAGKDWQ | ||||||
Alternative sequence | VSP_060583 | 280-282 | in isoform 6 | |||
Sequence: ALC → GGAPSPQRQADRTTPGGRRSTAQHQVGQR |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK092539 EMBL· GenBank· DDBJ | BAC03912.1 EMBL· GenBank· DDBJ | mRNA | ||
AK096344 EMBL· GenBank· DDBJ | BAC04765.1 EMBL· GenBank· DDBJ | mRNA | ||
AK096802 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
DQ500957 EMBL· GenBank· DDBJ | ABF47100.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC120024 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471099 EMBL· GenBank· DDBJ | EAW89607.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471099 EMBL· GenBank· DDBJ | EAW89605.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471099 EMBL· GenBank· DDBJ | EAW89615.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC037889 EMBL· GenBank· DDBJ | AAH37889.1 EMBL· GenBank· DDBJ | mRNA | ||
BC045824 EMBL· GenBank· DDBJ | AAH45824.1 EMBL· GenBank· DDBJ | mRNA | ||
BC064545 EMBL· GenBank· DDBJ | AAH64545.1 EMBL· GenBank· DDBJ | mRNA |