Q8N7N5 · DCAF8_MOUSE
- ProteinDDB1- and CUL4-associated factor 8
- GeneDcaf8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids591 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Miscellaneous
The homozygous loop-tail (Lp) mouse has a severe neural tube closure defect, analogous to the craniorachischisis phenotype seen in humans. This gene has been mapped to The Lp critical region.
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Cul4-RING E3 ubiquitin ligase complex | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Biological Process | protein ubiquitination |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDDB1- and CUL4-associated factor 8
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8N7N5
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000296958 | 1-591 | DDB1- and CUL4-associated factor 8 | |||
Sequence: MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS | ||||||
Modified residue | 22 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 23 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 100 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 123 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 124 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 198 | Omega-N-methylarginine; by PRMT1 | ||||
Sequence: R |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the brain.
Developmental stage
Expressed in the brain at 8.5 dpc, 9.5 dpc and 10.5 dpc.
Gene expression databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, motif, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-26 | Polar residues | ||||
Sequence: MSNKRPNTTDGRTDLANGSLSSSPEE | ||||||
Region | 1-140 | Disordered | ||||
Sequence: MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTA | ||||||
Motif | 40-51 | Nuclear export signal | ||||
Sequence: IEVEASDLSLSL | ||||||
Compositional bias | 52-68 | Polar residues | ||||
Sequence: TGDDGGPNRTSTESRGT | ||||||
Compositional bias | 69-133 | Basic and acidic residues | ||||
Sequence: DTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDW | ||||||
Repeat | 185-224 | WD 1 | ||||
Sequence: GHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLD | ||||||
Repeat | 228-269 | WD 2 | ||||
Sequence: GHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKN | ||||||
Repeat | 275-315 | WD 3 | ||||
Sequence: QHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASK | ||||||
Repeat | 323-363 | WD 4 | ||||
Sequence: EKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENEN | ||||||
Repeat | 379-418 | WD 5 | ||||
Sequence: ESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQY | ||||||
Repeat | 426-466 | WD 6 | ||||
Sequence: RNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQF | ||||||
Repeat | 470-509 | WD 7 | ||||
Sequence: DKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTEL | ||||||
Region | 552-591 | Disordered | ||||
Sequence: HRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS |
Sequence similarities
Belongs to the WD repeat DCAF8 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8N7N5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length591
- Mass (Da)66,031
- Last updated2002-10-01 v1
- Checksum2FFC175E1E2D1F4A
Q8N7N5-2
- Name2
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0A6YX55 | A0A0A6YX55_MOUSE | Dcaf8 | 138 | ||
A0A0A6YWY2 | A0A0A6YWY2_MOUSE | Dcaf8 | 153 |
Sequence caution
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-26 | Polar residues | ||||
Sequence: MSNKRPNTTDGRTDLANGSLSSSPEE | ||||||
Compositional bias | 52-68 | Polar residues | ||||
Sequence: TGDDGGPNRTSTESRGT | ||||||
Compositional bias | 69-133 | Basic and acidic residues | ||||
Sequence: DTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDW | ||||||
Alternative sequence | VSP_027266 | 376-411 | in isoform 2 | |||
Sequence: VNSESKANITCLVYSHDGTELLASYNDEDIYLFNSS → SSWPVTMMKTFTFSTLLTVMGPSILRDTKAIEIMLQ | ||||||
Alternative sequence | VSP_027267 | 412-591 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK098114 EMBL· GenBank· DDBJ | BAC05237.1 EMBL· GenBank· DDBJ | mRNA | ||
AK146825 EMBL· GenBank· DDBJ | BAE27462.1 EMBL· GenBank· DDBJ | mRNA | ||
BC023704 EMBL· GenBank· DDBJ | AAH23704.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BC023804 EMBL· GenBank· DDBJ | AAH23804.1 EMBL· GenBank· DDBJ | mRNA |