Q8MMJ7 · NDB41_OPIMA
- ProteinCytotoxic linear peptide IsCT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cytotoxic linear peptide IsCT
Shows weak hemolytic activity and antibacterial activity against both Gram-positive and Gram-negative bacteria probably by forming pores in the cell membrane. IsCT adopts an amphipathic alpha-helical structure.
Cytotoxic linear peptide IsCTf
Shows neither hemolytic, nor antibacterial activities, probably because it cannot adopt amphipathic alpha-helical structure.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 29 | Important for antibacterial activity, and hemolysis activity | ||||
Sequence: W |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | membrane | |
Cellular Component | other organism cell membrane | |
Molecular Function | toxin activity | |
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism | |
Biological Process | monoatomic ion transport |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameCytotoxic linear peptide IsCT
- Alternative names
- Cleaved into 1 chains
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Scorpiones > Iurida > Scorpionoidea > Hemiscorpiidae > Opisthacanthus
Accessions
- Primary accessionQ8MMJ7
Subcellular Location
UniProt Annotation
GO Annotation
Note: Forms a helical membrane channel in the prey.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 29 | Drastic reduction in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 29 | Drastic reduction in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity; when associated with K-34. | ||||
Sequence: W → L | ||||||
Mutagenesis | 30 | Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and little loss of hemolysis activity. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity; when associated with P-31 and K-34. | ||||
Sequence: E → K | ||||||
Mutagenesis | 31 | Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity; when associated with K-30 and K-34. | ||||
Sequence: G → P | ||||||
Mutagenesis | 34 | Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity; when associated with L-29. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis; when associated with K-30 and P-31. | ||||
Sequence: S → K |
PTM/Processing
Features
Showing features for signal, peptide, modified residue, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-23 | |||||
Sequence: MKTQFAILLVALVLFQMFAQSDA | ||||||
Peptide | PRO_0000035357 | 24-34 | Cytotoxic linear peptide IsCTf | |||
Sequence: ILGKIWEGIKS | ||||||
Peptide | PRO_0000035356 | 24-36 | Cytotoxic linear peptide IsCT | |||
Sequence: ILGKIWEGIKSLF | ||||||
Modified residue | 36 | Phenylalanine amide | ||||
Sequence: F | ||||||
Propeptide | PRO_0000035358 | 40-68 | ||||
Sequence: GLSDLDGLDELFDGEISKADRDFLRELMR |
Post-translational modification
IsCTf is an enzymatic proteolytic cleavage product of IsCT by the proteases present in the venom.
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Belongs to the non-disulfide-bridged peptide (NDBP) superfamily. Short antimicrobial peptide (group 4) family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length68
- Mass (Da)7,722
- Last updated2002-10-01 v1
- ChecksumFAFBC86E52583297
Mass Spectrometry
Cytotoxic linear peptide IsCT
Molecular mass is 1,502 Da. Determined by MALDI.Cytotoxic linear peptide IsCT
Molecular mass is 1,502.9 Da. Determined by MALDI.Cytotoxic linear peptide IsCTf
Molecular mass is 1,242.8 Da. Determined by MALDI.Keywords
- Technical term