Q8MMJ7 · NDB41_OPIMA

Function

function

Cytotoxic linear peptide IsCT

Shows weak hemolytic activity and antibacterial activity against both Gram-positive and Gram-negative bacteria probably by forming pores in the cell membrane. IsCT adopts an amphipathic alpha-helical structure.

Cytotoxic linear peptide IsCTf

Shows neither hemolytic, nor antibacterial activities, probably because it cannot adopt amphipathic alpha-helical structure.

Features

Showing features for site.

1685101520253035404550556065
MKTQFAILLVALVLFQMFAQSDAILGKIWEGIKSLFGKRGLSDLDGLDELFDGEISKADRDFLRELMR
TypeIDPosition(s)Description
Site29Important for antibacterial activity, and hemolysis activity

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componentmembrane
Cellular Componentother organism cell membrane
Molecular Functiontoxin activity
Biological Processdefense response to bacterium
Biological Processkilling of cells of another organism
Biological Processmonoatomic ion transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Cytotoxic linear peptide IsCT
  • Alternative names
    • Non-disulfide-bridged peptide 4.1
      (NDBP-4.1
      )
    • Non-disulfide-bridged peptide 5.2
      (NDBP-5.2
      )
  • Cleaved into 1 chains

Organism names

Accessions

  • Primary accession
    Q8MMJ7

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis29Drastic reduction in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity.
Mutagenesis29Drastic reduction in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity; when associated with K-34.
Mutagenesis30Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and little loss of hemolysis activity. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity; when associated with P-31 and K-34.
Mutagenesis31Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis activity; when associated with K-30 and K-34.
Mutagenesis34Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity; when associated with L-29. Similar or 2-fold increase in antibacterial activity against both Gram-positive and Gram-negative bacteria, and complete loss of hemolysis; when associated with K-30 and P-31.

PTM/Processing

Features

Showing features for signal, peptide, modified residue, propeptide.

TypeIDPosition(s)Description
Signal1-23
PeptidePRO_000003535724-34Cytotoxic linear peptide IsCTf
PeptidePRO_000003535624-36Cytotoxic linear peptide IsCT
Modified residue36Phenylalanine amide
PropeptidePRO_000003535840-68

Post-translational modification

IsCTf is an enzymatic proteolytic cleavage product of IsCT by the proteases present in the venom.

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    68
  • Mass (Da)
    7,722
  • Last updated
    2002-10-01 v1
  • Checksum
    FAFBC86E52583297
MKTQFAILLVALVLFQMFAQSDAILGKIWEGIKSLFGKRGLSDLDGLDELFDGEISKADRDFLRELMR

Mass Spectrometry

Cytotoxic linear peptide IsCT

Molecular mass is 1,502 Da. Determined by MALDI.

Cytotoxic linear peptide IsCT

Molecular mass is 1,502.9 Da. Determined by MALDI.

Cytotoxic linear peptide IsCTf

Molecular mass is 1,242.8 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF397895
EMBL· GenBank· DDBJ
AAM76913.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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