Q8LPN3 · ADCS_ARATH

Function

function

Bifunctional enzyme that catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. In the first step, a glutamine amidotransferase generates ammonia that is channelled between the binding sites of glutamine and chorismate and used along with chorismate in the second step, catalyzed by aminodeoxychorismate synthase, to produce ADC. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis. Does not possess ADC lyase activity.

Catalytic activity

Activity regulation

Activated by chorismate and inhibited by dihydrofolate and methotrexate.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
600 μML-glutamine
1.5 μMchorismate

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site172Nucleophile
Active site316
Active site318

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Function4-amino-4-deoxychorismate synthase activity
Biological Processchorismate metabolic process
Biological Processfolic acid biosynthetic process
Biological Processglutamine metabolic process
Biological Processpara-aminobenzoic acid biosynthetic process
Biological Processtetrahydrofolate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aminodeoxychorismate synthase, chloroplastic
  • EC number
  • Short names
    ADC synthase
  • Alternative names
    • P-aminobenzoic acid synthase (PABA synthase)
    • Para-aminobenzoate synthase
    • Protein EMBRYO DEFFECTIVE 1997

Gene names

    • Name
      ADCS
    • Synonyms
      EMB1997
    • ORF names
      F8N16.17
    • Ordered locus names
      At2g28880

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8LPN3
  • Secondary accessions
    • Q9ZV26

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-45Chloroplast
ChainPRO_000043015446-919Aminodeoxychorismate synthase, chloroplastic

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain86-342Glutamine amidotransferase type-1
Region436-910PABB component

Domain

The PABA component provides the glutamine amidotransferase activity.
The PABB component catalyzes the formation of ADC by binding chorismate and ammonia.

Sequence similarities

In the C-terminal section; belongs to the anthranilate synthase component I family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    919
  • Mass (Da)
    102,920
  • Last updated
    2002-10-01 v1
  • Checksum
    4DC9792D1C3D111D
MNMNFSFCSTSSELSYPSENVLRFSVASRLFSPKWKKSFISLPCRSKTTRKVLASSRYVPGKLEDLSVVKKSLPRREPVEKLGFVRTLLIDNYDSYTFNIYQALSTINGVPPVVIRNDEWTWEEAYHYLYEDVAFDNIVISPGPGSPMCPADIGICLRLLLECRDIPILGVCLGHQALGYVHGAHVVHAPEPVHGRLSGIEHDGNILFSDIPSGRNSDFKVVRYHSLIIDKESLPKELVPIAWTIYDDTGSFSEKNSCVPVNNTGSPLGNGSVIPVSEKLENRSHWPSSHVNGKQDRHILMGIMHSSFPHYGLQFHPESIATTYGSQLFKNFKDITVNYWSRCKSTSLRRRNINDTANMQVPDATQLLKELSRTRCTGNGSSYFGNPKSLFSAKTNGVDVFDMVDSSYPKPHTKLLRLKWKKHERLAHKVGGVRNIFMELFGKNRGNDTFWLDTSSSDKARGRFSFMGGKGGSLWKQLTFSLSDQSEVTSKHAGHLLIEDSQSSTEKQFLEEGFLDFLRKELSSISYDEKDFEELPFDFCGGYVGCIGYDIKVECGMPINRHKSNAPDACFFFADNVVAIDHQLDDVYILSLYEEGTAETSFLNDTEEKLISLMGLSTRKLEDQTLPVIDSSQSKTSFVPDKSREQYINDVQSCMKYIKDGESYELCLTTQNRRKIGNADPLGLYLHLRERNPAPYAAFLNFSNANLSLCSSSPERFLKLDRNGMLEAKPIKGTIARGSTPEEDEFLKLQLKLSEKNQAENLMIVDLLRNDLGRVCEPGSVHVPNLMDVESYTTVHTMVSTIRGLKKTDISPVECVRAAFPGGSMTGAPKLRSVEILDSLENCSRGLYSGSIGYFSYNGTFDLNIVIRTVIIHEDEASIGAGGAIVALSSPEDEFEEMILKTRAPANAVMEFCSDQRRQ

Sequence caution

The sequence AAC79592.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BK001419
EMBL· GenBank· DDBJ
DAA01457.1
EMBL· GenBank· DDBJ
mRNA
AC005727
EMBL· GenBank· DDBJ
AAC79592.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
CP002685
EMBL· GenBank· DDBJ
AEC08184.1
EMBL· GenBank· DDBJ
Genomic DNA
AY096797
EMBL· GenBank· DDBJ
AAM19955.1
EMBL· GenBank· DDBJ
mRNA
BT002702
EMBL· GenBank· DDBJ
AAO11618.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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