Q8LPN3 · ADCS_ARATH
- ProteinAminodeoxychorismate synthase, chloroplastic
- GeneADCS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids919 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Bifunctional enzyme that catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. In the first step, a glutamine amidotransferase generates ammonia that is channelled between the binding sites of glutamine and chorismate and used along with chorismate in the second step, catalyzed by aminodeoxychorismate synthase, to produce ADC. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis. Does not possess ADC lyase activity.
Catalytic activity
- chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate
Activity regulation
Activated by chorismate and inhibited by dihydrofolate and methotrexate.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
600 μM | L-glutamine | |||||
1.5 μM | chorismate |
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 172 | Nucleophile | |||
Active site | 316 | ||||
Active site | 318 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | 4-amino-4-deoxychorismate synthase activity | |
Biological Process | chorismate metabolic process | |
Biological Process | folic acid biosynthetic process | |
Biological Process | glutamine metabolic process | |
Biological Process | para-aminobenzoic acid biosynthetic process | |
Biological Process | tetrahydrofolate biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminodeoxychorismate synthase, chloroplastic
- EC number
- Short namesADC synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8LPN3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-45 | Chloroplast | |||
Chain | PRO_0000430154 | 46-919 | Aminodeoxychorismate synthase, chloroplastic | ||
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 86-342 | Glutamine amidotransferase type-1 | |||
Region | 436-910 | PABB component | |||
Domain
The PABA component provides the glutamine amidotransferase activity.
The PABB component catalyzes the formation of ADC by binding chorismate and ammonia.
Sequence similarities
In the C-terminal section; belongs to the anthranilate synthase component I family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length919
- Mass (Da)102,920
- Last updated2002-10-01 v1
- Checksum4DC9792D1C3D111D
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BK001419 EMBL· GenBank· DDBJ | DAA01457.1 EMBL· GenBank· DDBJ | mRNA | ||
AC005727 EMBL· GenBank· DDBJ | AAC79592.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
CP002685 EMBL· GenBank· DDBJ | AEC08184.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY096797 EMBL· GenBank· DDBJ | AAM19955.1 EMBL· GenBank· DDBJ | mRNA | ||
BT002702 EMBL· GenBank· DDBJ | AAO11618.1 EMBL· GenBank· DDBJ | mRNA |