Q8LL04 · CPL3_ARATH

Function

function

Completely dephosphorylates 'Ser-2', and partially 'Ser-5' and 'Ser-7' of the heptad repeats YSPTSPS in the C-terminal domain (CTD) of the largest RNA polymerase II subunit (RPB1) (PubMed:25464831).
Involved in defense response (PubMed:25464831).
Acts as a negative regulator of immune gene expression and immunity to pathogen infections (PubMed:25464831).
Preferentially dephosphorylates 'Ser-2' of RNA polymerase II CTD (PubMed:25464831).
This counterregulates the MAP kinase (MAPK) or cyclin-dependent kinase C (CDKC)-mediated phosphorylation of CTD in response to pathogens and upon perception of microbe-associated molecular patterns (MAMPs) (PubMed:25464831).
MAPKs phosphorylate and activate CDKCs, which are CTD kinases that positively regulate plant innate immunity (PubMed:25464831).
Acts as a negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated signaling pathway and cold resistance (PubMed:12149434, PubMed:16905668).
Acts as a post-transcriptional gene silencing (PTGS) suppressor (PubMed:31076735).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Co2+ (UniProtKB | Rhea| CHEBI:48828 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds Mg2+, Co2+ or Mn2+.

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA polymerase II CTD heptapeptide repeat phosphatase activity
Biological Processdefense response
Biological Processnegative regulation of abscisic acid-activated signaling pathway
Biological Processnegative regulation of post-transcriptional gene silencing by regulatory ncRNA
Biological Processresponse to salt stress

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA polymerase II C-terminal domain phosphatase-like 3
  • EC number
  • Short names
    FCP-like 3
  • Alternative names
    • Carboxyl-terminal phosphatase-like 3
      (AtCPL3
      ; CTD phosphatase-like 3
      )

Gene names

    • Name
      CPL3
    • Synonyms
      AGGIE1
    • ORF names
      F4P9.31
    • Ordered locus names
      At2g33540

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8LL04
  • Secondary accessions
    • O22804

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Grows more slowly and flower earlier than wild-type plants. ABA hyperactivation of stress-inducible genes.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis933Loss of phosphatase activity.
Mutagenesis1064Loss of phosphatase activity; when associated with A-1065.
Mutagenesis1065Loss of phosphatase activity; when associated with A-1064.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 57 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003760851-1241RNA polymerase II C-terminal domain phosphatase-like 3

Proteomic databases

PTM databases

Expression

Induction

By NaCl.

Gene expression databases

Interaction

Subunit

Interacts with RAP74.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region361-402Disordered
Compositional bias428-443Polar residues
Region428-470Disordered
Compositional bias450-468Polar residues
Region505-525Disordered
Region578-598Disordered
Region677-702Disordered
Region720-800Disordered
Compositional bias750-773Polar residues
Region852-885Disordered
Compositional bias863-885Polar residues
Domain923-1103FCP1 homology
Domain1146-1239BRCT

Domain

The BRCT domain is required for interaction with RAP74.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,241
  • Mass (Da)
    136,479
  • Last updated
    2009-05-26 v2
  • Checksum
    CEFFBD725719D404
MLVARSGCSRTLIRMGNDENLMVMVDVEEGEIPDSVNTEIEVKHKSTTTTADVGGDVDVGVVAGGRGGGGGGSNGNSRVWTMEELISQYPAYRPYANSGLSNLAWARAVQNKPFNEGLVMDYEPRESDKIVIEDSDDEKEEGELEEGEIDLVDNASDDNLVEKDTESVVLISADKVEDDRILKERDLEKKVKLIRGVLESTSLVEAQTGFEGVCSRILGALESLRELVSDNDDFPKRDTLVQLSFASLQTINYVFCSMNNISKERNKETMSRLLTLVNDHFSQFLSFNQKNEIETMNQDLSRSAIAVFAGTSSEENVNQMTQPSNGDSFLAKKLTSESTHRGAAYLRSRLPMLPLLDLHKDHDADSLPSPTRETTPSLPVNGRHTMVRPGFPVGRESQTTEGAKVYSYESDARKAVSTYQQKFGLNSVFKTDDLPSPTPSGEPNDGNGDVGGEVSSSVVKSSNPGSHLIYGQDVPLPSNFNSRSMPVANSVSSTVPPHHLSIHAISAPTASDQTVKPSAKSRDPRLRLAKPDAANVTIYSYSSGDARNLSKVELSADLVNPRKQKAADEFLIDGPAWKRQKSDTDAPKAAGTGGWLEDTESSGLLKLESKPRLIENGVTSMTSSVMPTSAVSVSQKVRTASTDTASLQSLLKDIAVNPTMLLNLLKMGERQKVPEKAIQKPMDPRRAAQLPGSSVQPGVSTPLSIPASNALAANSLNSGVLQDSSQNAPAAESGSIRMKPRDPRRILHGSTLQRTDSSMEKQTKVNDPSTLGTLTMKGKAEDLETPPQLDPRQNISQNGTSKMKISGELLSGKTPDFSTQFTKNLKSIADMVVVSQQLGNPPASMHSVQLKTERDVKHNPSNPNAQDEDVSVSAASVTAAAGPTRSMNSWGDVEHLFEGYDDIQRVAIQRERVRRLEEQNKMFASQKLSLVLDIDHTLLNSAKFNEVESRHEEILRKKEEQDREKPYRHLFRFLHMGMWTKLRPGIWNFLEKASKLYELHLYTMGNKLYATEMAKLLDPKGVLFNGRVISKGDDGDPLDGDERVPKSKDLEGVMGMESSVVIIDDSVRVWPQHKMNLIAVERYLYFPCSRRQFGLLGPSLLELDRDEVPEEGTLASSLAVIEKIHQNFFSHTSLDEVDVRNILASEQRKILAGCRIVFSRIIPVGEAKPHLHPLWQTAEQFGAVCTTQVDEHVTHVVTNSLGTDKVNWALTRGRFVVHPGWVEASAFLYQRANENLYAINP

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8B2G0A0A1P8B2G0_ARATHCPL3984

Sequence caution

The sequence AAB80671.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias428-443Polar residues
Compositional bias450-468Polar residues
Compositional bias750-773Polar residues
Compositional bias863-885Polar residues
Sequence conflict1010in Ref. 1; AAM94371

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF486633
EMBL· GenBank· DDBJ
AAM94371.1
EMBL· GenBank· DDBJ
mRNA
AC002332
EMBL· GenBank· DDBJ
AAB80671.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002685
EMBL· GenBank· DDBJ
AEC08850.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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