Q8LEC0 · RLPHL_ARATH
- ProteinTyrosine-protein phosphatase At3g09960
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids311 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score2/5
Function
function
Protein phosphatase that dephosphorylates specifically tyrosine-phosphorylated peptides; especially active on dual-phosphorylated substrates containing a phosphothreonine-X-phosphotyrosine motif.
Catalytic activity
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
Cofactor
Note: Binds 2 divalent metal cations per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 14 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 16 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 16 | substrate | ||||
Sequence: H | ||||||
Binding site | 48 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 48 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 52 | substrate | ||||
Sequence: R | ||||||
Binding site | 81 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 82 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 133 | substrate | ||||
Sequence: R | ||||||
Binding site | 215 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 240 | substrate | ||||
Sequence: R | ||||||
Binding site | 247 | substrate | ||||
Sequence: R | ||||||
Binding site | 268 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | metal ion binding | |
Molecular Function | protein tyrosine phosphatase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTyrosine-protein phosphatase At3g09960
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8LEC0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000447244 | 1-311 | Tyrosine-protein phosphatase At3g09960 | |||
Sequence: MMTQKPRTVICVGDIHGNISKLNKLWLNLQSDIQNSDFSSALVIFLGDYCDRGPETRKVIDFLISLPEKHPDQTHVFLAGNHDFAFAGFLGLLPRPSDGSEFKETWKEYSKSEEREGWYKGEGFENMHLQSRRWAGKIRVQFDYSAYGVLYNGSIYDAASTFESYGVPHGSSDLIKAVPESHKKFLTNMVWVHKEDDVCIETEEGLTHCKLIAVHAGLETKNNVEEQLKLLRDKDTSIPRIQPLTGRKTVWGIPQELDDKKTIVVSGHHGKLHIDGLRLIIDEGGGYTDTPLAAIVLPSKKIIRDTDNFSN |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length311
- Mass (Da)35,085
- Last updated2002-10-01 v1
- Checksum0573DA23565E56F9
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010927 EMBL· GenBank· DDBJ | AAF04427.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AC015985 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002686 EMBL· GenBank· DDBJ | AEE74841.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY085511 EMBL· GenBank· DDBJ | AAM62735.1 EMBL· GenBank· DDBJ | mRNA |