Q8LDN8 · UGE3_ARATH

Function

function

Catalyzes the interconversion between UDP-glucose and UDP-galactose and the interconversion between UDP-arabinose and UDP-xylose. Cooperates with UGE2 in pollen development. May preferentially act in the UDP-galactose to UDP-glucose direction, therefore displaying a role in carbohydrate catabolism (Probable).

Catalytic activity

Cofactor

NAD+ (UniProtKB | Rhea| CHEBI:57540 )

Activity regulation

Strongly inhibited by UDP.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.19 mMUDP-glucose
0.19 mMUDP-galactose
0.22 mMUDP-galactose
0.068 mMUDP-galactose
0.32 mMUDP-xylose
kcat is 19 sec-1 with UDP-glucose as substrate (PubMed:16644739).
kcat is 42 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 57 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 66 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 27 sec-1 with UDP-galactose as substrate (PubMed:19754426).
kcat is 13 sec-1 with UDP-xylose as substrate (PubMed:19754426).

pH Dependence

Optimum pH is 7.0-9.0.

Temperature Dependence

Optimum temperature is 35 degrees Celsius.

Pathway

Carbohydrate metabolism; galactose metabolism.
Nucleotide-sugar biosynthesis; UDP-L-arabinose biosynthesis; UDP-L-arabinose from UDP-alpha-D-xylose: step 1/1.
Cell wall biogenesis; cell wall polysaccharide biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site8-39NAD+ (UniProtKB | ChEBI)
Binding site134substrate
Active site158Proton acceptor

GO annotations

AspectTerm
Molecular Functionprotein dimerization activity
Molecular FunctionUDP-arabinose 4-epimerase activity
Molecular FunctionUDP-glucose 4-epimerase activity
Biological Processcapsule polysaccharide biosynthetic process
Biological Processcell wall organization
Biological Processgalactose metabolic process
Biological Processpollen development
Biological ProcessUDP-L-arabinose biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3
  • EC number
  • Alternative names
    • UDP-D-xylose 4-epimerase
    • UDP-L-arabinose 4-epimerase
    • UDP-galactose 4-epimerase 3
    • UDP-glucose 4-epimerase 3 (AtUGE3
      )

Gene names

    • Name
      UGE3
    • ORF names
      F16M19.8
    • Ordered locus names
      At1g63180

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8LDN8
  • Secondary accessions
    • Q9CAM5

Proteomes

Organism-specific databases

Genome annotation databases

Phenotypes & Variants

Disruption phenotype

No visible phenotype. Uge2 and uge3 double mutant is almost completely sterile (PubMed:17496119).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004221851-351Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3

Proteomic databases

Expression

Tissue specificity

Ubiquitous.

Induction

Down-regulated by ethylene.

Gene expression databases

Interaction

Subunit

homodimer. Heterodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    351
  • Mass (Da)
    38,910
  • Last updated
    2002-10-01 v1
  • Checksum
    314BC1FA8DD091EF
MGSSVEQNILVTGGAGFIGTHTVVQLLNQGFKVTIIDNLDNSVVEAVHRVRELVGPDLSTKLEFNLGDLRNKGDIEKLFSNQRFDAVIHFAGLKAVGESVGNPRRYFDNNLVGTINLYETMAKYNCKMMVFSSSATVYGQPEIVPCVEDFELQAMNPYGRTKLFLEEIARDIHAAEPEWKIILLRYFNPVGAHESGRIGEDPKGIPNNLMPYIQQVAVGRLPELNVFGHDYPTMDGSAVRDYIHVMDLADGHVAALNKLFSDSKIGCTAYNLGTGQGTSVLEMVSSFEKASGKKIPIKLCPRRAGDATAVYASTQKAEKELGWKAKYGVDEMCRDQWNWANKNPWGFQKKP

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8APY6A0A1P8APY6_ARATHUGE3304

Sequence caution

The sequence AAG51599.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC010795
EMBL· GenBank· DDBJ
AAG51599.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002684
EMBL· GenBank· DDBJ
AEE34065.1
EMBL· GenBank· DDBJ
Genomic DNA
AK117913
EMBL· GenBank· DDBJ
BAC42551.1
EMBL· GenBank· DDBJ
mRNA
BT024882
EMBL· GenBank· DDBJ
ABD85153.1
EMBL· GenBank· DDBJ
mRNA
AY085887
EMBL· GenBank· DDBJ
AAM63099.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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