Q8LDN8 · UGE3_ARATH
- ProteinBifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3
- GeneUGE3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids351 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the interconversion between UDP-glucose and UDP-galactose and the interconversion between UDP-arabinose and UDP-xylose. Cooperates with UGE2 in pollen development. May preferentially act in the UDP-galactose to UDP-glucose direction, therefore displaying a role in carbohydrate catabolism (Probable).
Catalytic activity
- UDP-alpha-D-glucose = UDP-alpha-D-galactose
Cofactor
Activity regulation
Strongly inhibited by UDP.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.19 mM | UDP-glucose | |||||
0.19 mM | UDP-galactose | |||||
0.22 mM | UDP-galactose | |||||
0.068 mM | UDP-galactose | |||||
0.32 mM | UDP-xylose |
kcat is 19 sec-1 with UDP-glucose as substrate (PubMed:16644739).
kcat is 42 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 57 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 66 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 27 sec-1 with UDP-galactose as substrate (PubMed:19754426).
kcat is 13 sec-1 with UDP-xylose as substrate (PubMed:19754426).
kcat is 42 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 57 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 66 sec-1 with UDP-galactose as substrate (PubMed:16644739).
kcat is 27 sec-1 with UDP-galactose as substrate (PubMed:19754426).
kcat is 13 sec-1 with UDP-xylose as substrate (PubMed:19754426).
pH Dependence
Optimum pH is 7.0-9.0.
Temperature Dependence
Optimum temperature is 35 degrees Celsius.
Pathway
Carbohydrate metabolism; galactose metabolism.
Nucleotide-sugar biosynthesis; UDP-L-arabinose biosynthesis; UDP-L-arabinose from UDP-alpha-D-xylose: step 1/1.
Cell wall biogenesis; cell wall polysaccharide biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | protein dimerization activity | |
Molecular Function | UDP-arabinose 4-epimerase activity | |
Molecular Function | UDP-glucose 4-epimerase activity | |
Biological Process | capsule polysaccharide biosynthetic process | |
Biological Process | cell wall organization | |
Biological Process | galactose metabolic process | |
Biological Process | pollen development | |
Biological Process | UDP-L-arabinose biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8LDN8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
No visible phenotype. Uge2 and uge3 double mutant is almost completely sterile (PubMed:17496119).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000422185 | 1-351 | Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3 | |||
Sequence: MGSSVEQNILVTGGAGFIGTHTVVQLLNQGFKVTIIDNLDNSVVEAVHRVRELVGPDLSTKLEFNLGDLRNKGDIEKLFSNQRFDAVIHFAGLKAVGESVGNPRRYFDNNLVGTINLYETMAKYNCKMMVFSSSATVYGQPEIVPCVEDFELQAMNPYGRTKLFLEEIARDIHAAEPEWKIILLRYFNPVGAHESGRIGEDPKGIPNNLMPYIQQVAVGRLPELNVFGHDYPTMDGSAVRDYIHVMDLADGHVAALNKLFSDSKIGCTAYNLGTGQGTSVLEMVSSFEKASGKKIPIKLCPRRAGDATAVYASTQKAEKELGWKAKYGVDEMCRDQWNWANKNPWGFQKKP |
Proteomic databases
Expression
Tissue specificity
Ubiquitous.
Induction
Down-regulated by ethylene.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length351
- Mass (Da)38,910
- Last updated2002-10-01 v1
- Checksum314BC1FA8DD091EF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8APY6 | A0A1P8APY6_ARATH | UGE3 | 304 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010795 EMBL· GenBank· DDBJ | AAG51599.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE34065.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK117913 EMBL· GenBank· DDBJ | BAC42551.1 EMBL· GenBank· DDBJ | mRNA | ||
BT024882 EMBL· GenBank· DDBJ | ABD85153.1 EMBL· GenBank· DDBJ | mRNA | ||
AY085887 EMBL· GenBank· DDBJ | AAM63099.1 EMBL· GenBank· DDBJ | mRNA |