Q8L799 · MIOX1_ARATH
- ProteinInositol oxygenase 1
- GeneMIOX1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids311 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the oxygenative cleavage of myo-inositol to D-glucuronate. Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.
Catalytic activity
- myo-inositol + O2 = D-glucuronate + H2O + H+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 iron ions per subunit.
Pathway
Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 52 | substrate | |||
Binding site | 109-111 | substrate | |||
Binding site | 122 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 147 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 148 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 148 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 151 | substrate | |||
Binding site | 168-169 | substrate | |||
Binding site | 220 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 246 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 246-247 | substrate | |||
Binding site | 279 | Fe cation 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | inositol oxygenase activity | |
Molecular Function | iron ion binding | |
Biological Process | inositol catabolic process | |
Biological Process | L-ascorbic acid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInositol oxygenase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8L799
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Incorporation of the inositol pathway-derived monosaccharides is strongly reduced in knockout AtMIOX1 seedling walls.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 22 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000079154 | 1-311 | Inositol oxygenase 1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in roots, young leaves, stems, flowers and siliques.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-29 | Disordered | |||
Compositional bias | 9-29 | Basic and acidic residues | |||
Sequence similarities
Belongs to the myo-inositol oxygenase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q8L799-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length311
- Mass (Da)36,574
- Last updated2002-10-01 v1
- Checksum0BCBDE175D505F59
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8APC9 | A0A1P8APC9_ARATH | MIOX1 | 341 | ||
Q3EDC6 | Q3EDC6_ARATH | At1g14630 | 226 | ||
A0A1P8AWY2 | A0A1P8AWY2_ARATH | At1g14630 | 166 | ||
F4HW72 | F4HW72_ARATH | MIOX1 | 309 |
Sequence caution
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 9-29 | Basic and acidic residues | |||
Sequence conflict | 111 | in Ref. 5; BAC42925 and 6; BAD43581/BAD43596/BAD94364 | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010657 EMBL· GenBank· DDBJ | AAF63180.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC012188 EMBL· GenBank· DDBJ | AAF43953.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE29174.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM58508.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY136388 EMBL· GenBank· DDBJ | AAM97054.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000187 EMBL· GenBank· DDBJ | AAN15506.1 EMBL· GenBank· DDBJ | mRNA | ||
AK118307 EMBL· GenBank· DDBJ | BAC42925.1 EMBL· GenBank· DDBJ | mRNA | ||
AK175115 EMBL· GenBank· DDBJ | BAD42878.1 EMBL· GenBank· DDBJ | mRNA | ||
AK175818 EMBL· GenBank· DDBJ | BAD43581.1 EMBL· GenBank· DDBJ | mRNA | ||
AK175833 EMBL· GenBank· DDBJ | BAD43596.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176690 EMBL· GenBank· DDBJ | BAD44453.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221931 EMBL· GenBank· DDBJ | BAD94364.1 EMBL· GenBank· DDBJ | mRNA |