Q8L707 · IRX14_ARATH

Function

function

Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls (PubMed:17944810, PubMed:20335400, PubMed:20424005, PubMed:20595206).
Involved in the elongation of glucuronoxylan xylosyl backbone (PubMed:17944810, PubMed:20335400, PubMed:20424005, PubMed:20595206).
Xylan xylosyltransferase that acts cooperatively with IRX9 to achieve the successive addition of xylosyl residues during xylan backbone elongation (PubMed:22080591, PubMed:25118690).
Required for the proper composition and structural properties of released seed coat mucilage (PubMed:26482889, PubMed:26834178).
Required for the production of highly branched xylan polymers in seed coat mucilage (PubMed:26482889, PubMed:26834178).
Xylan with xylose side chains seems to be necessary for pectin attachment to the seed surface (PubMed:26482889, PubMed:26834178).
Together with MUCI70, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells (PubMed:26482889, PubMed:26834178, PubMed:30228108).

Catalytic activity

GO annotations

AspectTerm
Cellular Componentendosome
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular ComponentGolgi trans cisterna
Cellular Componenttrans-Golgi network
Molecular Function1,4-beta-D-xylan synthase activity
Molecular Functiongalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
Molecular Functionxylosyltransferase activity
Biological Processfruit development
Biological Processglucuronoxylan biosynthetic process
Biological Processmucilage biosynthetic process involved in seed coat development
Biological Processmucilage extrusion from seed coat
Biological Processmucilage pectin biosynthetic process
Biological Processrhamnogalacturonan I biosynthetic process
Biological Processshoot system development
Biological Processxylan biosynthetic process
Biological Processxylan metabolic process
Biological Processxylem and phloem pattern formation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-1,4-xylosyltransferase IRX14
  • EC number
  • Alternative names
    • Protein IRREGULAR XYLEM 14
    • Xylan xylosyltransferase IRX14

Gene names

    • Name
      IRX14
    • ORF names
      AP22.51
      , C7A10.470
    • Ordered locus names
      At4g36890

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8L707
  • Secondary accessions
    • O23194

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-35Cytoplasmic
Transmembrane36-56Helical; Signal-anchor for type II membrane protein
Topological domain57-525Lumenal

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced xylan content in cell wall (PubMed:17944810).
Reduced amount and altered composition of seed coat mucilage (PubMed:26482889, PubMed:26834178).
Plants missing both MUCI70 and IRX14 exhibit a severe reduction in both xylan- and pectin-related sugars in total seed mucilage extracts (PubMed:30228108).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis261-262Unable to complement irregular xylem phenotype of irx14 mutant.
Mutagenesis321Complements irregular xylem phenotype of irx14 mutant.
Mutagenesis415Complements irregular xylem phenotype of irx14 mutant.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 24 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004075651-525Beta-1,4-xylosyltransferase IRX14
Glycosylation102N-linked (GlcNAc...) asparagine
Glycosylation204N-linked (GlcNAc...) asparagine
Glycosylation326N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in developing interfascicular fibers and xylem cells in stems and developing secondary xylem in roots.

Developmental stage

Expressed in the seed coat at the linear cotyledon and mature green stages, when mucilage synthesis occurs.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region452-525Disordered
Compositional bias469-484Polar residues
Compositional bias488-502Basic residues

Sequence similarities

Belongs to the glycosyltransferase 43 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    525
  • Mass (Da)
    59,099
  • Last updated
    2002-10-01 v1
  • Checksum
    025BFCC9A2C1C348
MKLSALHQSYLNRRSNSFRSPTSLDSSVDGSGKSLIAVFWLILHCLCCLISLVLGFRFSRLVFFFLFSTSSTNLYSLPFRPDLPVKHLDVHTIGRTLDPGANGTTVVATATKSSRVVVGRHGIRIRPWPHPNPVEVMKAHQIIGRVQKEQKMIFGMKSSKMVIAVTPTYVRTFQALHLTGVMHSLMLVPYDLVWIVVEAGGATNETGLIIAKSGLRTIHVGIDQRMPNTWEDRSKLEVFMRLQALRVVREEKLDGIVMFADDSNMHSMELFDEIQNVKWFGTVSVGILAHSGNAEEMVLSMEKRKEMEKEEEEESSSLPVQGPACNSTDQLIGWHIFNTLPYAGKSAVYIDDVAAVLPQKLEWSGFVLNSRLLWEEAENKPEWVKDFGSLNENEGVESPLSLLKDPSMVEPLGSCGRQVLLWWLRVEARADSKFPPGWIIDPPLEITVAAKRTPWPDVPPEPPTKKKDQMPLSQGNTVVVIPKQQQHPTKIRKPKRKSKKSKHEPRPTDTTTQVYSSSSKHQERN

Sequence caution

The sequence CAB16800.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB80355.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias469-484Polar residues
Compositional bias488-502Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z99707
EMBL· GenBank· DDBJ
CAB16800.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161590
EMBL· GenBank· DDBJ
CAB80355.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE86714.1
EMBL· GenBank· DDBJ
Genomic DNA
AY140036
EMBL· GenBank· DDBJ
AAM98177.1
EMBL· GenBank· DDBJ
mRNA
BT014969
EMBL· GenBank· DDBJ
AAT47820.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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