Q8L707 · IRX14_ARATH
- ProteinBeta-1,4-xylosyltransferase IRX14
- GeneIRX14
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids525 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls (PubMed:17944810, PubMed:20335400, PubMed:20424005, PubMed:20595206).
Involved in the elongation of glucuronoxylan xylosyl backbone (PubMed:17944810, PubMed:20335400, PubMed:20424005, PubMed:20595206).
Xylan xylosyltransferase that acts cooperatively with IRX9 to achieve the successive addition of xylosyl residues during xylan backbone elongation (PubMed:22080591, PubMed:25118690).
Required for the proper composition and structural properties of released seed coat mucilage (PubMed:26482889, PubMed:26834178).
Required for the production of highly branched xylan polymers in seed coat mucilage (PubMed:26482889, PubMed:26834178).
Xylan with xylose side chains seems to be necessary for pectin attachment to the seed surface (PubMed:26482889, PubMed:26834178).
Together with MUCI70, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells (PubMed:26482889, PubMed:26834178, PubMed:30228108).
Involved in the elongation of glucuronoxylan xylosyl backbone (PubMed:17944810, PubMed:20335400, PubMed:20424005, PubMed:20595206).
Xylan xylosyltransferase that acts cooperatively with IRX9 to achieve the successive addition of xylosyl residues during xylan backbone elongation (PubMed:22080591, PubMed:25118690).
Required for the proper composition and structural properties of released seed coat mucilage (PubMed:26482889, PubMed:26834178).
Required for the production of highly branched xylan polymers in seed coat mucilage (PubMed:26482889, PubMed:26834178).
Xylan with xylose side chains seems to be necessary for pectin attachment to the seed surface (PubMed:26482889, PubMed:26834178).
Together with MUCI70, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells (PubMed:26482889, PubMed:26834178, PubMed:30228108).
Catalytic activity
- [(1->4)-beta-D-xylan](n) + UDP-alpha-D-xylose = [(1->4)-beta-D-xylan](n+1) + H+ + UDPThis reaction proceeds in the forward direction.
[(1→4)-β-D-xylan](n) RHEA-COMP:9548 + CHEBI:57632 = [(1→4)-β-D-xylan](n+1) RHEA-COMP:9549 + CHEBI:15378 + CHEBI:58223
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endosome | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Cellular Component | Golgi trans cisterna | |
Cellular Component | trans-Golgi network | |
Molecular Function | 1,4-beta-D-xylan synthase activity | |
Molecular Function | galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity | |
Molecular Function | xylosyltransferase activity | |
Biological Process | fruit development | |
Biological Process | glucuronoxylan biosynthetic process | |
Biological Process | mucilage biosynthetic process involved in seed coat development | |
Biological Process | mucilage extrusion from seed coat | |
Biological Process | mucilage pectin biosynthetic process | |
Biological Process | rhamnogalacturonan I biosynthetic process | |
Biological Process | shoot system development | |
Biological Process | xylan biosynthetic process | |
Biological Process | xylan metabolic process | |
Biological Process | xylem and phloem pattern formation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-1,4-xylosyltransferase IRX14
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8L707
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-35 | Cytoplasmic | ||||
Sequence: MKLSALHQSYLNRRSNSFRSPTSLDSSVDGSGKSL | ||||||
Transmembrane | 36-56 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: IAVFWLILHCLCCLISLVLGF | ||||||
Topological domain | 57-525 | Lumenal | ||||
Sequence: RFSRLVFFFLFSTSSTNLYSLPFRPDLPVKHLDVHTIGRTLDPGANGTTVVATATKSSRVVVGRHGIRIRPWPHPNPVEVMKAHQIIGRVQKEQKMIFGMKSSKMVIAVTPTYVRTFQALHLTGVMHSLMLVPYDLVWIVVEAGGATNETGLIIAKSGLRTIHVGIDQRMPNTWEDRSKLEVFMRLQALRVVREEKLDGIVMFADDSNMHSMELFDEIQNVKWFGTVSVGILAHSGNAEEMVLSMEKRKEMEKEEEEESSSLPVQGPACNSTDQLIGWHIFNTLPYAGKSAVYIDDVAAVLPQKLEWSGFVLNSRLLWEEAENKPEWVKDFGSLNENEGVESPLSLLKDPSMVEPLGSCGRQVLLWWLRVEARADSKFPPGWIIDPPLEITVAAKRTPWPDVPPEPPTKKKDQMPLSQGNTVVVIPKQQQHPTKIRKPKRKSKKSKHEPRPTDTTTQVYSSSSKHQERN |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Reduced xylan content in cell wall (PubMed:17944810).
Reduced amount and altered composition of seed coat mucilage (PubMed:26482889, PubMed:26834178).
Plants missing both MUCI70 and IRX14 exhibit a severe reduction in both xylan- and pectin-related sugars in total seed mucilage extracts (PubMed:30228108).
Reduced amount and altered composition of seed coat mucilage (PubMed:26482889, PubMed:26834178).
Plants missing both MUCI70 and IRX14 exhibit a severe reduction in both xylan- and pectin-related sugars in total seed mucilage extracts (PubMed:30228108).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 261-262 | Unable to complement irregular xylem phenotype of irx14 mutant. | ||||
Sequence: DD → AA | ||||||
Mutagenesis | 321 | Complements irregular xylem phenotype of irx14 mutant. | ||||
Sequence: Q → A | ||||||
Mutagenesis | 415 | Complements irregular xylem phenotype of irx14 mutant. | ||||
Sequence: C → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 24 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000407565 | 1-525 | Beta-1,4-xylosyltransferase IRX14 | |||
Sequence: MKLSALHQSYLNRRSNSFRSPTSLDSSVDGSGKSLIAVFWLILHCLCCLISLVLGFRFSRLVFFFLFSTSSTNLYSLPFRPDLPVKHLDVHTIGRTLDPGANGTTVVATATKSSRVVVGRHGIRIRPWPHPNPVEVMKAHQIIGRVQKEQKMIFGMKSSKMVIAVTPTYVRTFQALHLTGVMHSLMLVPYDLVWIVVEAGGATNETGLIIAKSGLRTIHVGIDQRMPNTWEDRSKLEVFMRLQALRVVREEKLDGIVMFADDSNMHSMELFDEIQNVKWFGTVSVGILAHSGNAEEMVLSMEKRKEMEKEEEEESSSLPVQGPACNSTDQLIGWHIFNTLPYAGKSAVYIDDVAAVLPQKLEWSGFVLNSRLLWEEAENKPEWVKDFGSLNENEGVESPLSLLKDPSMVEPLGSCGRQVLLWWLRVEARADSKFPPGWIIDPPLEITVAAKRTPWPDVPPEPPTKKKDQMPLSQGNTVVVIPKQQQHPTKIRKPKRKSKKSKHEPRPTDTTTQVYSSSSKHQERN | ||||||
Glycosylation | 102 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 204 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 326 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in developing interfascicular fibers and xylem cells in stems and developing secondary xylem in roots.
Developmental stage
Expressed in the seed coat at the linear cotyledon and mature green stages, when mucilage synthesis occurs.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 452-525 | Disordered | ||||
Sequence: RTPWPDVPPEPPTKKKDQMPLSQGNTVVVIPKQQQHPTKIRKPKRKSKKSKHEPRPTDTTTQVYSSSSKHQERN | ||||||
Compositional bias | 469-484 | Polar residues | ||||
Sequence: QMPLSQGNTVVVIPKQ | ||||||
Compositional bias | 488-502 | Basic residues | ||||
Sequence: PTKIRKPKRKSKKSK |
Sequence similarities
Belongs to the glycosyltransferase 43 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length525
- Mass (Da)59,099
- Last updated2002-10-01 v1
- Checksum025BFCC9A2C1C348
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 469-484 | Polar residues | ||||
Sequence: QMPLSQGNTVVVIPKQ | ||||||
Compositional bias | 488-502 | Basic residues | ||||
Sequence: PTKIRKPKRKSKKSK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z99707 EMBL· GenBank· DDBJ | CAB16800.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161590 EMBL· GenBank· DDBJ | CAB80355.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE86714.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY140036 EMBL· GenBank· DDBJ | AAM98177.1 EMBL· GenBank· DDBJ | mRNA | ||
BT014969 EMBL· GenBank· DDBJ | AAT47820.1 EMBL· GenBank· DDBJ | mRNA |