Q8L5V0 · RPB9B_ARATH
- ProteinDNA-directed RNA polymerases II, IV and V subunit 9B
- GeneNRPB9B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids114 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Required for RNA silencing.
Miscellaneous
The loss of silencing in nrpb9b mutants is due to a defect in Pol V function, but not at the level of transcription. Pol IV functions are not impaired in nrpb9b mutants and Pol II functions are partially complemented by NRPB9A (PubMed:22550619).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 7 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 10 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 29 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 32 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 76 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 79 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 103 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 108 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Cellular Component | RNA polymerase II, core complex | |
Cellular Component | RNA polymerase IV complex | |
Cellular Component | RNA polymerase V complex | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | nucleic acid binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription | |
Biological Process | gene silencing by siRNA-directed DNA methylation |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerases II, IV and V subunit 9B
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8L5V0
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Shorter siliques and ovate leaves with shorter petioles, smaller trichomes, prominent leaf veins and changed cuticular wax coating. Loss of methylation at RdDM target sites. Partially redundant with NRPB9A with respect to Pol II. Nrpb9a and nrpb9b double mutants are embryo lethal.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 5 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423337 | 1-114 | DNA-directed RNA polymerases II, IV and V subunit 9B | |||
Sequence: MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILSDVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 72-113 | TFIIS-type | ||||
Sequence: KAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWR |
Sequence similarities
Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length114
- Mass (Da)13,107
- Last updated2002-10-01 v1
- ChecksumFF5D0ADD2977757F
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8B3U3 | A0A1P8B3U3_ARATH | NRPB9B | 133 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL117321 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002687 EMBL· GenBank· DDBJ | AEE83723.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT028953 EMBL· GenBank· DDBJ | ABI49500.1 EMBL· GenBank· DDBJ | mRNA | ||
AY084946 EMBL· GenBank· DDBJ | AAM61507.1 EMBL· GenBank· DDBJ | mRNA |