Q8L5V0 · RPB9B_ARATH

Function

function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Required for RNA silencing.

Miscellaneous

The loss of silencing in nrpb9b mutants is due to a defect in Pol V function, but not at the level of transcription. Pol IV functions are not impaired in nrpb9b mutants and Pol II functions are partially complemented by NRPB9A (PubMed:22550619).

Features

Showing features for binding site.

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TypeIDPosition(s)Description
Binding site7Zn2+ 1 (UniProtKB | ChEBI)
Binding site10Zn2+ 1 (UniProtKB | ChEBI)
Binding site29Zn2+ 1 (UniProtKB | ChEBI)
Binding site32Zn2+ 1 (UniProtKB | ChEBI)
Binding site76Zn2+ 2 (UniProtKB | ChEBI)
Binding site79Zn2+ 2 (UniProtKB | ChEBI)
Binding site103Zn2+ 2 (UniProtKB | ChEBI)
Binding site108Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleolus
Cellular ComponentRNA polymerase II, core complex
Cellular ComponentRNA polymerase IV complex
Cellular ComponentRNA polymerase V complex
Molecular FunctionDNA-directed 5'-3' RNA polymerase activity
Molecular Functionnucleic acid binding
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processgene silencing by siRNA-directed DNA methylation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed RNA polymerases II, IV and V subunit 9B

Gene names

    • Name
      NRPB9B
    • Synonyms
      NRPD9B, NRPE9B
    • ORF names
      FCAALL
    • Ordered locus names
      At4g16265

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8L5V0

Proteomes

Organism-specific databases

Genome annotation databases

Phenotypes & Variants

Disruption phenotype

Shorter siliques and ovate leaves with shorter petioles, smaller trichomes, prominent leaf veins and changed cuticular wax coating. Loss of methylation at RdDM target sites. Partially redundant with NRPB9A with respect to Pol II. Nrpb9a and nrpb9b double mutants are embryo lethal.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 5 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004233371-114DNA-directed RNA polymerases II, IV and V subunit 9B

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the RNA polymerase II, IV and V complexes. Interacts with NRPD1.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger72-113TFIIS-type

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    114
  • Mass (Da)
    13,107
  • Last updated
    2002-10-01 v1
  • Checksum
    FF5D0ADD2977757F
MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILSDVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8B3U3A0A1P8B3U3_ARATHNRPB9B133

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL117321
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CP002687
EMBL· GenBank· DDBJ
AEE83723.1
EMBL· GenBank· DDBJ
Genomic DNA
BT028953
EMBL· GenBank· DDBJ
ABI49500.1
EMBL· GenBank· DDBJ
mRNA
AY084946
EMBL· GenBank· DDBJ
AAM61507.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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