Q8L5J1 · MAN4_SOLLC
- ProteinMannan endo-1,4-beta-mannosidase 4
- GeneMAN4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids399 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Possesses endo-beta-mannanase and mannan transglycosylase activities. May be involved in cell wall degradation during fruit ripening.
Catalytic activity
pH Dependence
Optimum pH is 5.0 for both endo-beta-mannanase and mannan transglycosylase activities.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 88 | substrate | ||||
Sequence: W | ||||||
Binding site | 203 | substrate | ||||
Sequence: N | ||||||
Active site | 204 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 279 | substrate | ||||
Sequence: Y | ||||||
Active site | 318 | Nucleophile | ||||
Sequence: E | ||||||
Binding site | 360 | substrate | ||||
Sequence: W |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | mannan endo-1,4-beta-mannosidase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMannan endo-1,4-beta-mannosidase 4
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionQ8L5J1
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 394 | Activity slightly reduced. | ||||
Sequence: R → A | ||||||
Natural variant | 394-399 | in strain: cv. Castalia and cv. Walter; in allele LeMAN4i; inactive enzyme | ||||
Sequence: RLSKLS → AL | ||||||
Mutagenesis | 394-399 | Activity reduced by 5-fold. | ||||
Sequence: RLSKLS → ALS | ||||||
Mutagenesis | 396-399 | Activity reduced by 8-fold. | ||||
Sequence: Missing | ||||||
Mutagenesis | 397-399 | Activity reduced by 3-fold. | ||||
Sequence: Missing | ||||||
Mutagenesis | 398-399 | Activity reduced by 5-fold. | ||||
Sequence: Missing | ||||||
Mutagenesis | 399 | No effect on activity. | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-26 | |||||
Sequence: MNNSIILIFVAILIIFPNEFSKPTRA | ||||||
Chain | PRO_0000277492 | 27-399 | Mannan endo-1,4-beta-mannosidase 4 | |||
Sequence: FSNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTRIKVTNTFQQASKYKMNVARTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHLIMSLVNNWDAFGGKKQYVEWAVQRGQKLTSDDDFFTNPMVKGFYKNNVKVVLTRVNTITKVAYKDDPTILSWELINEPRCPSDLSGKTFQNWVLEMAGYLKSIDSNHLLEIGLEGFYGNDMRQYNPNSYIFGTNFISNNQVQGIDFTTIHMYPNQWLPGLTQEAQDKWASQWIQVHIDDSKMLKKPLLIAEFGKSTKTPGYTVAKRDNYFEKIYGTIFNCAKSGGPCGGGLFWQVLGQGMSSFDDGYQVVLQESPSTSRVILLQSLRLSKLS | ||||||
Disulfide bond | 347↔354 | |||||
Sequence: CAKSGGPC |
Keywords
- PTM
Proteomic databases
Expression
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length399
- Mass (Da)45,339
- Last updated2007-02-06 v2
- Checksum805A7D7866E5FF6B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 274 | in Ref. 2; AAK56557 | ||||
Sequence: T → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY046588 EMBL· GenBank· DDBJ | AAK97760.1 EMBL· GenBank· DDBJ | mRNA | ||
AY046589 EMBL· GenBank· DDBJ | AAK97759.2 EMBL· GenBank· DDBJ | mRNA | ||
AY034075 EMBL· GenBank· DDBJ | AAK56557.1 EMBL· GenBank· DDBJ | mRNA |