Q8L539 · 5FCL_ARATH

Function

function

Contributes to tetrahydrofolate metabolism and photorespiration through the regulation of serine hydroxymethyltransferase. Prefers the pentalutamyl to the monoglutamyl form of 5-formyltetrahydrofolate.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
5.8 μM5-formyltetrahydrofolate
35.2 μMATP
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
11.5 μmol/min/mgtoward 5-formyltetrahydrofolate

pH Dependence

Optimum pH is 8.5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site60-64ATP (UniProtKB | ChEBI)
Binding site113substrate
Binding site206-213ATP (UniProtKB | ChEBI)
Binding site207-211substrate
Binding site254ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrion
Molecular Function5-formyltetrahydrofolate cyclo-ligase activity
Molecular FunctionATP binding
Biological Processdefense response
Biological Processtetrahydrofolate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5-formyltetrahydrofolate cyclo-ligase, mitochondrial
  • EC number
  • Short names
    5-FCL

Gene names

    • Name
      5FCL
    • ORF names
      T19L5.10
    • Ordered locus names
      At5g13050

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8L539
  • Secondary accessions
    • F4K2F3
    • Q84WD9
    • Q9FYA5

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced growth rate and delayed flowering.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 18 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-48Mitochondrion
ChainPRO_000042871949-2775-formyltetrahydrofolate cyclo-ligase, mitochondrial

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q8L539-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    277
  • Mass (Da)
    31,233
  • Last updated
    2002-10-01 v1
  • Checksum
    221250EBE1D074FE
MIGARVFCITTTALRRSPIFFFPKIPTRPVFRLSPATRPIVAMSTTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSNTQKKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPITPRATESM

Q8L539-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence CAC05433.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_054249209-214in isoform 2
Alternative sequenceVSP_054250215-277in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF516365
EMBL· GenBank· DDBJ
AAM90960.1
EMBL· GenBank· DDBJ
mRNA
AL391711
EMBL· GenBank· DDBJ
CAC05433.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002688
EMBL· GenBank· DDBJ
AED91844.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED91845.1
EMBL· GenBank· DDBJ
Genomic DNA
BX830290
EMBL· GenBank· DDBJ
-mRNA No translation available.
BT024502
EMBL· GenBank· DDBJ
ABD19683.1
EMBL· GenBank· DDBJ
mRNA
AY084396
EMBL· GenBank· DDBJ
AAM60972.1
EMBL· GenBank· DDBJ
mRNA
BT003924
EMBL· GenBank· DDBJ
AAO41971.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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