Q8L539 · 5FCL_ARATH
- Protein5-formyltetrahydrofolate cyclo-ligase, mitochondrial
- Gene5FCL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids277 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Contributes to tetrahydrofolate metabolism and photorespiration through the regulation of serine hydroxymethyltransferase. Prefers the pentalutamyl to the monoglutamyl form of 5-formyltetrahydrofolate.
Catalytic activity
- (6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
5.8 μM | 5-formyltetrahydrofolate | |||||
35.2 μM | ATP |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
11.5 μmol/min/mg | toward 5-formyltetrahydrofolate |
pH Dependence
Optimum pH is 8.5.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | 5-formyltetrahydrofolate cyclo-ligase activity | |
Molecular Function | ATP binding | |
Biological Process | defense response | |
Biological Process | tetrahydrofolate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-formyltetrahydrofolate cyclo-ligase, mitochondrial
- EC number
- Short names5-FCL
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8L539
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Reduced growth rate and delayed flowering.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 18 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-48 | Mitochondrion | ||||
Sequence: MIGARVFCITTTALRRSPIFFFPKIPTRPVFRLSPATRPIVAMSTTSK | ||||||
Chain | PRO_0000428719 | 49-277 | 5-formyltetrahydrofolate cyclo-ligase, mitochondrial | |||
Sequence: NQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSNTQKKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPITPRATESM |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Structure
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q8L539-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length277
- Mass (Da)31,233
- Last updated2002-10-01 v1
- Checksum221250EBE1D074FE
Q8L539-2
- Name2
Sequence caution
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF516365 EMBL· GenBank· DDBJ | AAM90960.1 EMBL· GenBank· DDBJ | mRNA | ||
AL391711 EMBL· GenBank· DDBJ | CAC05433.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | AED91844.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED91845.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX830290 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BT024502 EMBL· GenBank· DDBJ | ABD19683.1 EMBL· GenBank· DDBJ | mRNA | ||
AY084396 EMBL· GenBank· DDBJ | AAM60972.1 EMBL· GenBank· DDBJ | mRNA | ||
BT003924 EMBL· GenBank· DDBJ | AAO41971.1 EMBL· GenBank· DDBJ | mRNA |