Q8KQL6 · PADGT_ACHLA

  • Protein
    Processive diacylglycerol alpha-glucosyltransferase
  • Gene
    dgs
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Processive glucosyltransferase involved in the biosynthesis of both the non-bilayer-prone alpha-monoglucosyldiacylglycerol and the bilayer-forming membrane lipid alpha-diglucosyldiacylglycerol. These are major components for maintaining the anionic lipid surface charge density, for balancing the bilayer to non-bilayer phase equilibria and for keeping a constant lipid bilayer spontaneous curvature (curvature packing stress). Catalyzes the transfer of a glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol), which then acts as acceptor to give alpha-diglucosyl-DAG product (3-O-(alpha-D-glucopyranosyl-alpha-(1->2)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). It can only use UDP-Glc as sugar donor.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Activated by the negatively charged lipids phosphatidylglycerol (PG), cardiolipin (CL), nonbilayer-prone 1,3-DAG, 1,2-dioleoylphosphatidylglycerol (DOPG) and 1,2-dioleoylphosphatidylserine (DOPS). Inhibited by 1,2-diacyl-3-O-(alpha-D-galactopyranosyl)-sn-glycerol, 1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.14 mMUDP-Glc28alpha-diglucosyldiacylglycerol synthase activity
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
19 nmol/h/mg28alpha-diglucosyldiacylglycerol synthase activity

Pathway

Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis.

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Function1,2-diacylglycerol 3-glucosyltransferase activity
Molecular Functiondiglucosyl diacylglycerol synthase activity
Molecular Functionmagnesium ion binding
Biological Processenterobacterial common antigen biosynthetic process
Biological Processglycerol metabolic process
Biological Processmembrane lipid biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT4Glycosyltransferase Family 4

Names & Taxonomy

Protein names

  • Recommended name
    Processive diacylglycerol alpha-glucosyltransferase
  • EC number
  • Alternative names
    • Alpha-diglucosyldiacylglycerol synthase (Alpha-DGS; DGlcDAG synthase)
    • Alpha-monoglucosyldiacylglycerol synthase (Alpha-MGS; MGlcDAG synthase)
    • Glucosyl-alpha-1,2-glucosyldiacylglycerol synthase
    • UDP-glucose:1,2-diacylglycerol 3-alpha-D-glucosyltransferase

Gene names

    • Name
      dgs

Organism names

  • Taxonomic identifier
  • Strain
    • A-EF22
  • Taxonomic lineage
    Bacteria > Mycoplasmatota > Mollicutes > Acholeplasmatales > Acholeplasmataceae > Acholeplasma

Accessions

  • Primary accession
    Q8KQL6

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004252741-332Processive diacylglycerol alpha-glucosyltransferase

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    332
  • Mass (Da)
    38,447
  • Last updated
    2002-10-01 v1
  • Checksum
    89D874EEF79E6793
MKVLLYSQKQSMLKKSGIGRAFYHQKRALEAVGIEYTTDPKDTYDLVHVNIAHSNKIKKFRKKYPVIVHGHSTVQDFRRSFAFWRVIAPFFYKHLQNIYGIADLIITPTRYSKFLIESMHVVKSPVVALSNGIDLDAYEYKQENVDAFRKHFDLEPNQKVVIGVGLLFERKGIHDFIEVARTMPNVTFIWFGNLSKLATTHFIRKRIKNKPKNMIMPGYVDGAVIKGAFSGADCVFFPSYEETEGIVVLEGLASKTPVVLRDIPVYYDWLFHKEHVLKGHNNFEFSKLIEKVLHEDQTEMIENGYKIVQDRSIEKIGEGLKQAYQEVIKIKR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY078412
EMBL· GenBank· DDBJ
AAL83700.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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