Q8KQL6 · PADGT_ACHLA
- ProteinProcessive diacylglycerol alpha-glucosyltransferase
- Genedgs
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids332 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Processive glucosyltransferase involved in the biosynthesis of both the non-bilayer-prone alpha-monoglucosyldiacylglycerol and the bilayer-forming membrane lipid alpha-diglucosyldiacylglycerol. These are major components for maintaining the anionic lipid surface charge density, for balancing the bilayer to non-bilayer phase equilibria and for keeping a constant lipid bilayer spontaneous curvature (curvature packing stress). Catalyzes the transfer of a glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol), which then acts as acceptor to give alpha-diglucosyl-DAG product (3-O-(alpha-D-glucopyranosyl-alpha-(1->2)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). It can only use UDP-Glc as sugar donor.
Catalytic activity
- a 1,2-diacyl-sn-glycerol + UDP-alpha-D-glucose = a 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + H+ + UDP
Cofactor
Activity regulation
Activated by the negatively charged lipids phosphatidylglycerol (PG), cardiolipin (CL), nonbilayer-prone 1,3-DAG, 1,2-dioleoylphosphatidylglycerol (DOPG) and 1,2-dioleoylphosphatidylserine (DOPS). Inhibited by 1,2-diacyl-3-O-(alpha-D-galactopyranosyl)-sn-glycerol, 1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.14 mM | UDP-Glc | 28 | alpha-diglucosyldiacylglycerol synthase activity |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
19 nmol/h/mg | 28 | alpha-diglucosyldiacylglycerol synthase activity |
Pathway
Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 1,2-diacylglycerol 3-glucosyltransferase activity | |
Molecular Function | diglucosyl diacylglycerol synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | enterobacterial common antigen biosynthetic process | |
Biological Process | glycerol metabolic process | |
Biological Process | membrane lipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProcessive diacylglycerol alpha-glucosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mollicutes > Acholeplasmatales > Acholeplasmataceae > Acholeplasma
Accessions
- Primary accessionQ8KQL6
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000425274 | 1-332 | Processive diacylglycerol alpha-glucosyltransferase | |||
Sequence: MKVLLYSQKQSMLKKSGIGRAFYHQKRALEAVGIEYTTDPKDTYDLVHVNIAHSNKIKKFRKKYPVIVHGHSTVQDFRRSFAFWRVIAPFFYKHLQNIYGIADLIITPTRYSKFLIESMHVVKSPVVALSNGIDLDAYEYKQENVDAFRKHFDLEPNQKVVIGVGLLFERKGIHDFIEVARTMPNVTFIWFGNLSKLATTHFIRKRIKNKPKNMIMPGYVDGAVIKGAFSGADCVFFPSYEETEGIVVLEGLASKTPVVLRDIPVYYDWLFHKEHVLKGHNNFEFSKLIEKVLHEDQTEMIENGYKIVQDRSIEKIGEGLKQAYQEVIKIKR |
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length332
- Mass (Da)38,447
- Last updated2002-10-01 v1
- Checksum89D874EEF79E6793
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY078412 EMBL· GenBank· DDBJ | AAL83700.1 EMBL· GenBank· DDBJ | Genomic DNA |