Q8KA62 · METF_BUCAP

Function

function

Catalyzes the NADH-dependent reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. Is required to provide the methyl group necessary for methionine synthetase to convert homocysteine to methionine; the methyl group is given by 5-methyltetrahydrofolate.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site28Proton donor/acceptor
Binding site59NADH (UniProtKB | ChEBI)
Binding site60FAD (UniProtKB | ChEBI)
Binding site62FAD (UniProtKB | ChEBI)
Binding site88FAD (UniProtKB | ChEBI)
Binding site118FAD (UniProtKB | ChEBI)
Binding site119FAD (UniProtKB | ChEBI)
Binding site120(6S)-5-methyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site120FAD (UniProtKB | ChEBI)
Binding site132FAD (UniProtKB | ChEBI)
Binding site152FAD (UniProtKB | ChEBI)
Binding site156FAD (UniProtKB | ChEBI)
Binding site165FAD (UniProtKB | ChEBI)
Binding site168FAD (UniProtKB | ChEBI)
Binding site171FAD (UniProtKB | ChEBI)
Binding site172FAD (UniProtKB | ChEBI)
Binding site183(6S)-5-methyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site183NADH (UniProtKB | ChEBI)
Binding site219(6S)-5-methyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site279(6S)-5-methyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionFAD binding
Molecular Functionmethylenetetrahydrofolate reductase (NADH) activity
Biological Processmethionine biosynthetic process
Biological Processtetrahydrofolate interconversion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5,10-methylenetetrahydrofolate reductase
  • EC number

Gene names

    • Name
      metF
    • Ordered locus names
      BUsg_043

Organism names

Accessions

  • Primary accession
    Q8KA62

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001902591-2925,10-methylenetetrahydrofolate reductase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    292
  • Mass (Da)
    33,676
  • Last updated
    2002-10-01 v1
  • Checksum
    0660895E1B065E71
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTYGANSGERKKTYSIVQRIHKKTGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGDVPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESKNAQSDILNLKKKADAGANRAITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFNGLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE013218
EMBL· GenBank· DDBJ
AAM67614.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help