Q8K4H1 · KFA_MOUSE

  • Protein
    Kynurenine formamidase
  • Gene
    Afmid
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.

Miscellaneous

The Afmid gene shares a bidirectional promoter region with Tk1 gene.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.18 mMN-formyl-L-kynurenine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
42 μmol/min/mg

Pathway

Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site162Nucleophile
Active site247
Active site279

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionarylformamidase activity
Biological Process'de novo' NAD biosynthetic process from tryptophan
Biological Processtryptophan catabolic process to kynurenine

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Kynurenine formamidase
  • EC number
  • Short names
    KFA
    ; KFase
  • Alternative names
    • Arylformamidase
    • N-formylkynurenine formamidase
      (FKF
      )

Gene names

    • Name
      Afmid

Organism names

  • Taxonomic identifier
  • Strains
    • Swiss Webster
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8K4H1
  • Secondary accessions
    • B1AQK9
    • Q80XP3
    • Q8R1K6

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm, cytosol
Nucleus
Note: Predominantly cytosolic. Some fraction is nuclear.

Keywords

Phenotypes & Variants

Disruption phenotype

Mice display sclerosis of kidney glomeruli, possibly due to failures in the elimination of toxic metabolites.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis162Loss of enzyme activity.
Mutagenesis247Loss of enzyme activity.
Mutagenesis279Loss of enzyme activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002483091-305Kynurenine formamidase

Post-translational modification

The N-terminus is blocked.

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in liver. Expressed in kidney. Weakly or not expressed in other tissues.

Induction

Down-regulated upon IL2-mediated activation. Transcriptional activation correlates with reduced histone acetylation.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif93-97HGGXW

Domain

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.

Sequence similarities

Belongs to the kynurenine formamidase family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q8K4H1-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    305
  • Mass (Da)
    34,229
  • Last updated
    2002-10-01 v1
  • Checksum
    898000A577132A52
MAFPSLSAGQNPWRNLSSEELEKQYSPSRWVIHTKPEEVVGNFVQIGSQATQKARATRRNQLDVPYGDGEGEKLDIYFPDEDSKAFPLFLFLHGGYWQSGSKDDSAFMVNPLTAQGIVVVIVAYDIAPKGTLDQMVDQVTRSVVFLQRRYPSNEGIYLCGHSAGAHLAAMVLLARWTKHGVTPNLQGFLLVSGIYDLEPLIATSQNDPLRMTLEDAQRNSPQRHLDVVPAQPVAPACPVLVLVGQHDSPEFHRQSKEFYETLLRVGWKASFQQLRGVDHFDIIENLTREDDVLTQIILKTVFQKL

Q8K4H1-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 296-305: IILKTVFQKL → VGLTPTTVFLNGQRGSHAHTWAWPGRQEQRERLAVPRWKSQSRLHRRACGECLLFIIVVAETMSHCITLAGLGLKI

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
B1AQL0B1AQL0_MOUSEAfmid363

Sequence caution

The sequence AAH24452.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict33in Ref. 4; AAH43309/AAH24452
Alternative sequenceVSP_020237296-305in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF399717
EMBL· GenBank· DDBJ
AAM62284.1
EMBL· GenBank· DDBJ
mRNA
AY099479
EMBL· GenBank· DDBJ
AAM44406.1
EMBL· GenBank· DDBJ
mRNA
AL591433
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC024452
EMBL· GenBank· DDBJ
AAH24452.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BC043309
EMBL· GenBank· DDBJ
AAH43309.1
EMBL· GenBank· DDBJ
mRNA
BC066780
EMBL· GenBank· DDBJ
AAH66780.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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