Q8K2H4 · ACAP1_MOUSE
- ProteinArf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
- GeneAcap1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids740 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).
Miscellaneous
Cells overexpressing Acap1 show accumulation of an electron dense coat containing Acap1 and Cltc on internal membranes as well as accumulation of Tfrc in pericentriolar recycling endosomes. Adipocytes with reduced level of Acap1 or Cltc fail to transport SLC2A4/GLUT4 from recycling endosomes to the cell surface upon insulin stimulation.
Activity regulation
GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | recycling endosome membrane | |
Molecular Function | GTPase activator activity | |
Molecular Function | metal ion binding | |
Biological Process | lipid catabolic process | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameArf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8K2H4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Recycling endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 448 | Loss of catalytic activity. No loss of accumulation of coat proteins on internal membranes upon overexpression of Acap1. | ||||
Sequence: R → Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 35 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000306384 | 1-740 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 | |||
Sequence: MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTHNAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEELNRLAQYRKELGTQLHNLVLNSARQKRDMEQRHVLLKQKELGGEEPEPSLKEGPSGLVMEGHLFKRASNAFKTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQLWVSAVQSSIASAFSQAHLENSPRGPGQVSGYHAPGSAATLACGGAARGRESGGVGQVAAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSECPSDDMGSLHPGALLFQAAGHPPSLPTMADALAHGADVNWVNVGQGNATPLIRATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDTEGRDPLTIAMETTNADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL | ||||||
Modified residue | 485 | 3'-nitrotyrosine | ||||
Sequence: Y |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, zinc finger, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-226 | BAR | ||||
Sequence: MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTHNAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEELNRLAQYRKELGTQLHN | ||||||
Region | 1-382 | Required for formation of endosomal tubules when overexpressed with PIP5K1C | ||||
Sequence: MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTHNAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEELNRLAQYRKELGTQLHNLVLNSARQKRDMEQRHVLLKQKELGGEEPEPSLKEGPSGLVMEGHLFKRASNAFKTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQLWVSAVQSSIASAFSQAHLENSPRGPGQVSGYH | ||||||
Domain | 265-360 | PH | ||||
Sequence: GLVMEGHLFKRASNAFKTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQLWVSAVQSSIA | ||||||
Domain | 405-527 | Arf-GAP | ||||
Sequence: GQVAAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIR | ||||||
Region | 405-740 | Required for interaction with GULP1 | ||||
Sequence: GQVAAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSECPSDDMGSLHPGALLFQAAGHPPSLPTMADALAHGADVNWVNVGQGNATPLIRATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDTEGRDPLTIAMETTNADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL | ||||||
Zinc finger | 420-443 | C4-type | ||||
Sequence: CCDCREPAPEWASINLGVTLCIQC | ||||||
Region | 525-562 | Disordered | ||||
Sequence: EIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSECP | ||||||
Region | 525-566 | Prevents interaction with ITGB1 when S-554 is not phosphorylated | ||||
Sequence: EIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSECPSDDM | ||||||
Compositional bias | 536-550 | Pro residues | ||||
Sequence: PRGHPPVPPKPPIRP | ||||||
Repeat | 606-635 | ANK 1 | ||||
Sequence: GNATPLIRATAANSLLACEFLLQNGANVNQ | ||||||
Repeat | 639-668 | ANK 2 | ||||
Sequence: AGRGPLHHATILGHTGLACLFLKRGADLGA | ||||||
Repeat | 672-702 | ANK 3 | ||||
Sequence: EGRDPLTIAMETTNADIVTLLRLAKMREAEA |
Domain
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers (By similarity).
The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions (By similarity).
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length740
- Mass (Da)81,704
- Last updated2002-10-01 v1
- Checksum798A87CBCC5F8513
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 522 | in Ref. 1; BAE32594 | ||||
Sequence: K → R | ||||||
Compositional bias | 536-550 | Pro residues | ||||
Sequence: PRGHPPVPPKPPIRP | ||||||
Sequence conflict | 584 | in Ref. 4; BAD90138 | ||||
Sequence: S → H |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK154449 EMBL· GenBank· DDBJ | BAE32594.1 EMBL· GenBank· DDBJ | mRNA | ||
AL596185 EMBL· GenBank· DDBJ | CAI35146.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL845465 EMBL· GenBank· DDBJ | CAI35146.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL845465 EMBL· GenBank· DDBJ | CAM13886.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL596185 EMBL· GenBank· DDBJ | CAM13886.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC031462 EMBL· GenBank· DDBJ | AAH31462.1 EMBL· GenBank· DDBJ | mRNA | ||
AK220213 EMBL· GenBank· DDBJ | BAD90138.1 EMBL· GenBank· DDBJ | mRNA |