Q8JZS7 · HMGC2_MOUSE
- Protein3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic
- GeneHmgcll1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids343 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Non-mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis, the products of which support energy production in nonhepatic animal tissues.
Catalytic activity
- (3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA
Cofactor
Pathway
Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56 | substrate | ||||
Sequence: R | ||||||
Binding site | 57 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 248 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 250 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 281 | |||||
Sequence: C | ||||||
Binding site | 290 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | membrane | |
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | hydroxymethylglutaryl-CoA lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | ketone body biosynthetic process | |
Biological Process | L-leucine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8JZS7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000334670 | 2-343 | 3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic | |||
Sequence: GNLPSAAKHCLNYQQLLREHLWSGDSVAGALDAAQEASQLPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQMADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEESMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQASFNARLE |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 48-315 | Pyruvate carboxyltransferase | ||||
Sequence: VKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQMADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEESMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVM |
Sequence similarities
Belongs to the HMG-CoA lyase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length343
- Mass (Da)36,650
- Last updated2002-10-01 v1
- Checksum1C9400381FB979DC
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK141622 EMBL· GenBank· DDBJ | BAE24772.1 EMBL· GenBank· DDBJ | mRNA | ||
BC037381 EMBL· GenBank· DDBJ | AAH37381.1 EMBL· GenBank· DDBJ | mRNA |