Q8IY34 · S15A3_HUMAN
- ProteinSolute carrier family 15 member 3
- GeneSLC15A3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids581 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Proton-coupled amino-acid transporter that transports free histidine and certain di- and tripeptides, and is involved in innate immune response (By similarity).
Also able to transport carnosine (PubMed:31073693, PubMed:31254495).
Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand (By similarity).
Also able to transport carnosine (PubMed:31073693, PubMed:31254495).
Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand (By similarity).
Catalytic activity
- glycylglycylglycine(out) + n H+(out) = glycylglycylglycine(in) + n H+(in)This reaction proceeds in the forward direction.glycylglycylglycine (out)CHEBI:195214
+ n H+ (out)CHEBI:15378= glycylglycylglycine (in)CHEBI:195214+ n H+ (in)CHEBI:15378 - carnosine(out) + n H+(out) = carnosine(in) + n H+(in)This reaction proceeds in the forward direction.
- n H+(out) + L-histidine(out) = n H+(in) + L-histidine(in)This reaction proceeds in the forward direction.
- n H+(out) + N-acetyl-D-muramoyl-L-alanyl-D-isoglutamine(out) = n H+(in) + N-acetyl-D-muramoyl-L-alanyl-D-isoglutamine(in)This reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
428 μM | glycyl-sarcosine | |||||
66.9 μM | L-histidine | |||||
5350 μM | valacyclovir |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1860 pmol/min/mg | toward L-histidine | ||||
73.6 pmol/min/mg | glycyl-sarcosine | ||||
63.7 pmol/min/mg | valacyclovir |
pH Dependence
Optimum pH is 5.5.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endosome membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | lysosomal membrane | |
Molecular Function | dipeptide transmembrane transporter activity | |
Molecular Function | peptide:proton symporter activity | |
Molecular Function | peptidoglycan transmembrane transporter activity | |
Biological Process | dipeptide import across plasma membrane | |
Biological Process | innate immune response | |
Biological Process | monoatomic ion transport | |
Biological Process | peptide transport | |
Biological Process | peptidoglycan transport | |
Biological Process | positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway | |
Biological Process | protein transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameSolute carrier family 15 member 3
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8IY34
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Lysosome membrane ; Multi-pass membrane protein
Endosome membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 38-58 | Helical | ||||
Sequence: VLLVEMLERAAFFGVTANLVL | ||||||
Transmembrane | 76-96 | Helical | ||||
Sequence: ALVFLGASYLLAPVGGWLADV | ||||||
Transmembrane | 103-123 | Helical | ||||
Sequence: AVALSLLLYLAASGLLPATAF | ||||||
Transmembrane | 155-175 | Helical | ||||
Sequence: PYCAPVLYAGLLLLGLAASSV | ||||||
Transmembrane | 200-220 | Helical | ||||
Sequence: NWFYWSINLGAVLSLLVVAFI | ||||||
Transmembrane | 232-252 | Helical | ||||
Sequence: IPVGCVGLAFFIFLFATPVFI | ||||||
Transmembrane | 310-330 | Helical | ||||
Sequence: FQVLVKILPVMVTLVPYWMVY | ||||||
Transmembrane | 369-389 | Helical | ||||
Sequence: TIPEAWLLLANVVVVLILVPL | ||||||
Transmembrane | 411-431 | Helical | ||||
Sequence: MALGMFFGFTSVIVAGVLEME | ||||||
Transmembrane | 458-478 | Helical | ||||
Sequence: IWWQIPQYLLIGISEIFASIP | ||||||
Transmembrane | 497-517 | Helical | ||||
Sequence: GIFFCLSGVGSLLGSSLVALL | ||||||
Transmembrane | 540-560 | Helical | ||||
Sequence: LYFFLLAGIQAVTALLFVWIA |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_034592 | 349 | in dbSNP:rs17855607 | |||
Sequence: I → F |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 680 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000295912 | 1-581 | Solute carrier family 15 member 3 | |||
Sequence: MPAPRAREQPRVPGERQPLLPRGARGPRRWRRAAGAAVLLVEMLERAAFFGVTANLVLYLNSTNFNWTGEQATRAALVFLGASYLLAPVGGWLADVYLGRYRAVALSLLLYLAASGLLPATAFPDGRSSFCGEMPASPLGPACPSAGCPRSSPSPYCAPVLYAGLLLLGLAASSVRSNLTSFGADQVMDLGRDATRRFFNWFYWSINLGAVLSLLVVAFIQQNISFLLGYSIPVGCVGLAFFIFLFATPVFITKPPMGSQVSSMLKLALQNCCPQLWQRHSARDRQCARVLADERSPQPGASPQEDIANFQVLVKILPVMVTLVPYWMVYFQMQSTYVLQGLHLHIPNIFPANPANISVALRAQGSSYTIPEAWLLLANVVVVLILVPLKDRLIDPLLLRCKLLPSALQKMALGMFFGFTSVIVAGVLEMERLHYIHHNETVSQQIGEVLYNAAPLSIWWQIPQYLLIGISEIFASIPGLEFAYSEAPRSMQGAIMGIFFCLSGVGSLLGSSLVALLSLPGGWLHCPKDFGNINNCRMDLYFFLLAGIQAVTALLFVWIAGRYERASQGPASHSRFSRDRG | ||||||
Glycosylation | 61 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 66 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 178 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 223 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 356 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 439 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8IY34 | HOXA1 P49639 | 3 | EBI-12179023, EBI-740785 | |
BINARY | Q8IY34 | KRTAP12-2 P59991 | 4 | EBI-12179023, EBI-10176379 | |
BINARY | Q8IY34 | KRTAP19-2 Q3LHN2 | 3 | EBI-12179023, EBI-12196745 | |
BINARY | Q8IY34 | KRTAP5-9 P26371 | 3 | EBI-12179023, EBI-3958099 | |
BINARY | Q8IY34 | KRTAP9-8 Q9BYQ0 | 3 | EBI-12179023, EBI-11958364 | |
BINARY | Q8IY34 | MDFI Q99750 | 3 | EBI-12179023, EBI-724076 | |
BINARY | Q8IY34 | NOD2 Q9HC29 | 2 | EBI-12179023, EBI-7445625 | |
BINARY | Q8IY34 | SLC15A4 Q8N697 | 2 | EBI-12179023, EBI-4319594 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-26 | Disordered | ||||
Sequence: MPAPRAREQPRVPGERQPLLPRGARG |
Sequence similarities
Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length581
- Mass (Da)63,560
- Last updated2010-05-18 v2
- Checksum56768D2963DB13D4
Computationally mapped potential isoform sequences
There are 9 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H0YFC1 | H0YFC1_HUMAN | SLC15A3 | 247 | ||
H0YG40 | H0YG40_HUMAN | SLC15A3 | 269 | ||
A0A7P0T8B5 | A0A7P0T8B5_HUMAN | SLC15A3 | 579 | ||
A0A7P0T8H0 | A0A7P0T8H0_HUMAN | SLC15A3 | 535 | ||
A0A7P0TAX8 | A0A7P0TAX8_HUMAN | SLC15A3 | 527 | ||
A0A7P0Z4G8 | A0A7P0Z4G8_HUMAN | SLC15A3 | 502 | ||
F5GZ36 | F5GZ36_HUMAN | SLC15A3 | 448 | ||
F5H251 | F5H251_HUMAN | SLC15A3 | 394 | ||
F5H1C8 | F5H1C8_HUMAN | SLC15A3 | 456 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB020598 EMBL· GenBank· DDBJ | BAA93432.1 EMBL· GenBank· DDBJ | mRNA | ||
AF135600 EMBL· GenBank· DDBJ | AAQ13565.1 EMBL· GenBank· DDBJ | mRNA | ||
AP003721 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC037974 EMBL· GenBank· DDBJ | AAH37974.1 EMBL· GenBank· DDBJ | mRNA |