Q8IPX7 · EXOS3_DROME
- ProteinExosome complex component RRP40
- GeneRrp40
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids232 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events (By similarity).
In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts such as antisense RNA species, and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm (By similarity).
In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs (By similarity).
The catalytic inactive RNA exosome core complex of 9 subunits is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes (By similarity).
Required generally for normal embryonic and neuronal development (PubMed:32645003).
Also plays a critical role in the maintenance of neuronal function in mature flies by controlling the levels of specific mRNAs such as the synaptic regulator Arc1 (PubMed:32645003).
In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts such as antisense RNA species, and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm (By similarity).
In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs (By similarity).
The catalytic inactive RNA exosome core complex of 9 subunits is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes (By similarity).
Required generally for normal embryonic and neuronal development (PubMed:32645003).
Also plays a critical role in the maintenance of neuronal function in mature flies by controlling the levels of specific mRNAs such as the synaptic regulator Arc1 (PubMed:32645003).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameExosome complex component RRP40
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ8IPX7
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown in the whole body, neurons, glia or muscles is lethal (PubMed:32645003).
RNAi-mediated knockdown in mushroom bodies affects the morphology of the alpha- and beta-lobes as well as ellipsoid bodies (PubMed:32645003).
RNAi-mediated knockdown in the wing pouch results in severe wing development phenotypes and increase of miR-252-5p expression (PubMed:25892215).
RNAi-mediated knockdown in mushroom bodies affects the morphology of the alpha- and beta-lobes as well as ellipsoid bodies (PubMed:32645003).
RNAi-mediated knockdown in the wing pouch results in severe wing development phenotypes and increase of miR-252-5p expression (PubMed:25892215).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 11 | Results in reduced viability, shortened lifespan, age-dependent wing posture and locomotor defects. Shows a mushroom body beta-lobe midline-crossing defect. Results in increased steady-state levels of specific neuronal mRNAs such as Arc1 and wfs1. | ||||
Sequence: G → A | ||||||
Mutagenesis | 87 | Results in reduced viability, shortened lifespan, age-dependent wing posture and locomotor defects. Results in increased steady-state levels of specific neuronal mRNAs such as Arc1 and wfs1. | ||||
Sequence: D → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451458 | 1-232 | Exosome complex component RRP40 | |||
Sequence: MSATSTIVMPGERIAAIEELAKSKRVILGPGLRRLDDTVVASKAGPLRHKEPGTFWVDNYQRRYIPARGDLILGIVRAKAGDLYRVDIGATDTASISYLAFEAASKKNRPDLIPGDLIYARVLNASADIEPELVCVNSVGKSGKLGVLTDGFFFKCSLNLGRMLLRENCPVLAALTRELPYEIAVGVNGRIWLKAHSLKETVALANAISALEQSGCAEIDKICGNLGDFLQA |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Component of the RNA exosome complex (PubMed:12490954).
Specifically part of the catalytically inactive RNA exosome core complex (By similarity).
Specifically part of the catalytically inactive RNA exosome core complex (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8IPX7 | Rrp46 Q9VGZ2 | 4 | EBI-106288, EBI-166198 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length232
- Mass (Da)25,026
- Last updated2003-03-01 v1
- Checksum5CCAB9DEECD84C7F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAN10459.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | AHN54080.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT023807 EMBL· GenBank· DDBJ | AAZ66314.1 EMBL· GenBank· DDBJ | mRNA |