Q8IKM8 · UCHL3_PLAF7

Function

function

Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8 (PubMed:17371404, PubMed:20042598, PubMed:31658303).
Essential for parasite blood stage survival (PubMed:20042598).

Catalytic activity

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    EC:3.4.19.12 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
301 nMubiquitin-AMC
kcat is 3.2 sec-1 with ubiquitin-AMC as substrate.

Features

Showing features for site, active site.

123220406080100120140160180200220
TypeIDPosition(s)Description
Site33Interaction with ubiquitin
Site86Transition state stabilizer
Active site92Nucleophile
Site157Interaction with ubiquitin
Active site164Proton donor
Site179Important for enzyme activity
Site219Interaction with ubiquitin

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentubiquitin ligase complex
Molecular Functioncysteine-type deubiquitinase activity
Molecular FunctiondeNEDDylase activity
Molecular Functionubiquitin binding
Biological Processprotein deneddylation
Biological Processprotein deubiquitination
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin carboxyl-terminal hydrolase UCHL3
  • EC number
  • Alternative names
    • PfUCHL3

Gene names

    • Name
      UCHL3
    • ORF names
      PF3D7_1460400

Organism names

  • Taxonomic identifier
  • Strain
    • 3D7
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Laverania)

Accessions

  • Primary accession
    Q8IKM8

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis13Small increase in deubiquitinating and deneddylating activities. Small reduction in deubiquitinating and deneddylating activities; when associated with E-33.
Mutagenesis33Small reduction in deubiquitinating and deneddylating activities. Small reduction in deubiquitinating and deneddylating activities; when associated with D-13.
Mutagenesis92Complete loss of deubiquitinating and deneddylating activities.

PTM/Processing

Features

Showing features for chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
ChainPRO_00004515721-232UniProtUbiquitin carboxyl-terminal hydrolase UCHL3
Modified residue (large scale data)114PTMeXchangePhosphoserine
Modified residue (large scale data)127PTMeXchangePhosphoserine
Modified residue (large scale data)140PTMeXchangePhosphoserine

Proteomic databases

Expression

Developmental stage

Expressed during the parasite blood stage, in schizonts (at protein level).

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain6-225UCH catalytic
Region10-14Interaction with ubiquitin
Region151-159Crossover loop which restricts access of large ubiquitin adducts to the active site
Region163-165Interaction with ubiquitin

Sequence similarities

Belongs to the peptidase C12 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    232
  • Mass (Da)
    26,899
  • Last updated
    2009-09-22 v2
  • Checksum
    8B2720342B644DFF
MAKNDIWTPLESNPDSLYLYSCKLGQSKLKFVDIYGFNNDLLDMIPQPVQAVIFLYPVNDNIVSENNTNDKHNLKENFDNVWFIKQYIPNSCGTIALLHLYGNLRNKFELDKDSVLDDFFNKVNEMSAEKRGQELKNNKSIENLHHEFCGQVENRDDILDVDTHFIVFVQIEGKIIELDGRKDHPTVHCFTNGDNFLYDTGKIIQDKFIEKCKDDLRFSALAVIPNDNFDII

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LN999946
EMBL· GenBank· DDBJ
CZU00300.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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