Q8I4M5 · GLS1_CAEEL

  • Protein
    Germline survival defective-1
  • Gene
    gls-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Required maternally for germline survival by forming a maternal complex with gld-3. During hermaphrodite development forms a complex with gld-3 which promotes the sperm/oocyte switch freeing the translational repressor fbf to turn off sperm promoting factors. Required for proper oocyte differentiation and oogenic meiotic arrest. Stimulates the enzymatic activity of gld-4 and together they prevent gld-1 mRNA degradation.

Miscellaneous

Trans-spliced exclusively to SL2, suggesting a transcriptional coregulation with the upstream gene dhfr-1.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular ComponentP granule
Molecular FunctionRNA polymerase II complex binding
Biological Processgerm-line sex determination
Biological Processmeiotic cell cycle
Biological ProcessmRNA 3'-end processing
Biological Processpositive regulation of gene expression
Biological Processregulation of translation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Germline survival defective-1

Gene names

    • Name
      gls-1
    • ORF names
      C36B1.8

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q8I4M5
  • Secondary accessions
    • C3UPA2
    • C6KRM2
    • Q93342

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cytoplasmic granule
Note: Localizes to P granules.

Keywords

Phenotypes & Variants

Disruption phenotype

Loss of maternal and zygotic expression causes germline survival defects. Zygotic mutants show a masculinized hermaphrodite germline. At 25 degress Celsius, hermaphrodite zygotic mutants show small oogenic cells that are either arrested in pachytene or have undergone abnormal meiotic prophase progression and failed to arrest in diakinesis, often resulting in endoreduplicating oocyte nuclei.

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-25
ChainPRO_000041808826-1052Germline survival defective-1

Proteomic databases

Expression

Tissue specificity

Expressed in the germline (at protein level). In the early embryo is expressed in all cells, then becomes gradually restricted to the germ cell lineage and enriches in P granules (at protein level). In adult hermaphrodites, is expressed in the mitotic region, accumulates during early stages of meiotic prophase I and is slightly less abundant in maturing oocytes (at protein level).

Developmental stage

Expressed both maternally and zygotically throughout development and in adult hermaphrodites (at protein level).

Gene expression databases

Interaction

Subunit

Isoform C interacts (via C-terminus) with gld-3 isoform A (via C-terminus) in an RNA-independent manner. Isoform C interacts with gld-4.

Binary interactions

TypeEntry 1
Entry 2Number of experimentsIntAct
BINARY Q8I4M5gld-3 Q95ZK7-13EBI-322416, EBI-14989519
BINARY Q8I4M5-3gld-3 Q95ZK7-16EBI-14989623, EBI-14989519

Complex viewer

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region41-320Disordered
Compositional bias163-172Polar residues
Compositional bias178-190Basic and acidic residues
Compositional bias305-320Basic and acidic residues
Region424-732gld-4 binding
Region478-543Disordered
Compositional bias480-514Polar residues
Region667-689Disordered
Compositional bias674-689Acidic residues
Region892-1052gld-3 binding
Region933-965Disordered
Region1033-1052Disordered

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Q8I4M5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,052
  • Mass (Da)
    116,014
  • Last updated
    2003-10-01 v2
  • MD5 Checksum
    2A06CC4B41F4960B0B3920FC3E03DB7E
MRCLISYLFHSFLIFLKFIRSDVTALTLQEKRKKSRLTGILMKSMANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDLLQETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Q8I4M5-2

  • Name
    a
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q8I4M5-3

  • Name
    c
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence ACO95124.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for alternative sequence, compositional bias.

Type
IDPosition(s)Description
Alternative sequenceVSP_0439671-44in isoform c
Compositional bias163-172Polar residues
Compositional bias178-190Basic and acidic residues
Compositional bias305-320Basic and acidic residues
Compositional bias480-514Polar residues
Compositional bias674-689Acidic residues
Alternative sequenceVSP_043968854-856in isoform a

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FJ610055
EMBL· GenBank· DDBJ
ACO95124.1
EMBL· GenBank· DDBJ
mRNA Different initiation
Z80215
EMBL· GenBank· DDBJ
CAB02273.3
EMBL· GenBank· DDBJ
Genomic DNA
Z80215
EMBL· GenBank· DDBJ
CAD56562.2
EMBL· GenBank· DDBJ
Genomic DNA
Z80215
EMBL· GenBank· DDBJ
CAZ65476.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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