Q8I4M5 · GLS1_CAEEL
- ProteinGermline survival defective-1
- Genegls-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1052 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required maternally for germline survival by forming a maternal complex with gld-3. During hermaphrodite development forms a complex with gld-3 which promotes the sperm/oocyte switch freeing the translational repressor fbf to turn off sperm promoting factors. Required for proper oocyte differentiation and oogenic meiotic arrest. Stimulates the enzymatic activity of gld-4 and together they prevent gld-1 mRNA degradation.
Miscellaneous
Trans-spliced exclusively to SL2, suggesting a transcriptional coregulation with the upstream gene dhfr-1.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | P granule | |
Molecular Function | RNA polymerase II complex binding | |
Biological Process | germ-line sex determination | |
Biological Process | meiotic cell cycle | |
Biological Process | mRNA 3'-end processing | |
Biological Process | positive regulation of gene expression | |
Biological Process | regulation of translation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGermline survival defective-1
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ8I4M5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to P granules.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Loss of maternal and zygotic expression causes germline survival defects. Zygotic mutants show a masculinized hermaphrodite germline. At 25 degress Celsius, hermaphrodite zygotic mutants show small oogenic cells that are either arrested in pachytene or have undergone abnormal meiotic prophase progression and failed to arrest in diakinesis, often resulting in endoreduplicating oocyte nuclei.
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-25 | ||||
Chain | PRO_0000418088 | 26-1052 | Germline survival defective-1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in the germline (at protein level). In the early embryo is expressed in all cells, then becomes gradually restricted to the germ cell lineage and enriches in P granules (at protein level). In adult hermaphrodites, is expressed in the mitotic region, accumulates during early stages of meiotic prophase I and is slightly less abundant in maturing oocytes (at protein level).
Developmental stage
Expressed both maternally and zygotically throughout development and in adult hermaphrodites (at protein level).
Gene expression databases
Interaction
Subunit
Isoform C interacts (via C-terminus) with gld-3 isoform A (via C-terminus) in an RNA-independent manner. Isoform C interacts with gld-4.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q8I4M5 | gld-3 Q95ZK7-1 | 3 | EBI-322416, EBI-14989519 | |
BINARY | Q8I4M5-3 | gld-3 Q95ZK7-1 | 6 | EBI-14989623, EBI-14989519 |
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 41-320 | Disordered | |||
Compositional bias | 163-172 | Polar residues | |||
Compositional bias | 178-190 | Basic and acidic residues | |||
Compositional bias | 305-320 | Basic and acidic residues | |||
Region | 424-732 | gld-4 binding | |||
Region | 478-543 | Disordered | |||
Compositional bias | 480-514 | Polar residues | |||
Region | 667-689 | Disordered | |||
Compositional bias | 674-689 | Acidic residues | |||
Region | 892-1052 | gld-3 binding | |||
Region | 933-965 | Disordered | |||
Region | 1033-1052 | Disordered | |||
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.
Q8I4M5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Nameb
- Length1,052
- Mass (Da)116,014
- Last updated2003-10-01 v2
- MD5 Checksum2A06CC4B41F4960B0B3920FC3E03DB7E
Q8I4M5-2
- Namea
- Differences from canonical
- 854-856: Missing
Q8I4M5-3
- Namec
- Differences from canonical
- 1-44: Missing
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_043967 | 1-44 | in isoform c | ||
Compositional bias | 163-172 | Polar residues | |||
Compositional bias | 178-190 | Basic and acidic residues | |||
Compositional bias | 305-320 | Basic and acidic residues | |||
Compositional bias | 480-514 | Polar residues | |||
Compositional bias | 674-689 | Acidic residues | |||
Alternative sequence | VSP_043968 | 854-856 | in isoform a | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ610055 EMBL· GenBank· DDBJ | ACO95124.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
Z80215 EMBL· GenBank· DDBJ | CAB02273.3 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z80215 EMBL· GenBank· DDBJ | CAD56562.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z80215 EMBL· GenBank· DDBJ | CAZ65476.1 EMBL· GenBank· DDBJ | Genomic DNA |