Q8H1F2 · RGS1_ARATH

Function

function

Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway (PubMed:26528314).
Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K+ and anion channels. Important for the kinetics of voltage activation of inward K+ current but not for the current amplitude.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentendosome membrane
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular FunctionG protein-coupled receptor activity
Molecular FunctionGTPase activator activity
Biological Processjasmonic acid mediated signaling pathway
Biological Processnegative regulation of signal transduction
Biological Processpositive regulation of GTPase activity
Biological Processregulation of cell population proliferation
Biological Processresponse to abscisic acid
Biological Processresponse to glucose
Biological Processresponse to water deprivation
Biological Processsugar mediated signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Regulator of G-protein signaling 1
  • Short names
    AtRGS1

Gene names

    • Name
      RGS1
    • ORF names
      MPE11.27, MPE11.28
    • Ordered locus names
      At3g26090

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8H1F2
  • Secondary accessions
    • Q8RWS5
    • Q9LU81
    • Q9LU82

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Endosome membrane
; Multi-pass membrane protein
Note: accumulates at the nascent cell plate during cytokinesis.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane9-29Helical
Transmembrane48-68Helical
Transmembrane83-103Helical
Transmembrane126-146Helical
Transmembrane157-177Helical
Transmembrane194-214Helical
Transmembrane226-246Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Increased cell elongation and longer hypocotyls when grown in the dark. Longer primary roots when grown in light due to increased cell production in root meristem. Loss of stratification requirement for seed germination. Decreased sensitivity to glucose and abscisic acid (ABA) in seed germination process (PubMed:26528314).
No effect on ABA inhibition of stomatal opening

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis320Loss of GTPase-accelerating activity, loss of interaction with GPA1 and loss of endocytosis.
Mutagenesis428Loss of endocytosis; when associated with A-435 and A-436.
Mutagenesis435Loss of endocytosis; when associated with A-428 and A-436.
Mutagenesis436Loss of endocytosis; when associated with A-428 and A-435.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 42 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004212641-459Regulator of G-protein signaling 1
Modified residue428Phosphoserine

Post-translational modification

Phosphorylated by WNK8 and WNK10, and in vitro by WNK1. Also phosphorylated at Ser-435 or Ser-436.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in hypocotyls, cotyledons, guard cells and root and shoot apical meristems.

Induction

Up-regulated by ozone.

Gene expression databases

Interaction

Subunit

Interacts (via C-ter) with GPA1 (PubMed:14500984, PubMed:17951432, PubMed:18817773, PubMed:21952135).
Interacts with WNK1, WNK8, WNK10, SYP23. The association with WNK8 at the plasma membrane is triggered by induction of glucose and continues with trafficking into endocytic compartments (PubMed:21952135).
Interacts with NDL1, NDL2 and NDL3 (PubMed:19948787).
Interacts with RHIP1 (PubMed:26528314).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q8H1F2GPA1 P180647EBI-1627025, EBI-443890

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain295-413RGS
Region429-459Disordered

Domain

The C-terminal domain (249-459) is able to bind to and to accelerate the GTPase activity of GPA1.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    459
  • Mass (Da)
    52,947
  • Last updated
    2003-03-01 v1
  • Checksum
    43AA65CD0417488E
MASGCALHGGCPSDYVAVAISVICFFVLLSRSVLPCLIHKAPRTNSSSFWIPVIQVISSFNLLFSIMMSVNLLRFRTKHWWRYCYLWAVWIEGPLGFGLLMSCRITQAFQLYFIFVKKRLPPVKSYIFLPLVLLPWIFGAAIIHATKPLNDKCHMGLQWTFPVAGLHALYVLALIAFTRAVRHVEFRFDELRDLWKGILVSATSIVIWVTAFVLNEIHEEISWLQVASRFVLLVTGGILVVVFFSISSNQPLLSQISLKKRQNFEFQRMGQALGIPDSGLLFRKEEFRPVDPNEPLDKLLLNKRFRHSFMEFADSCYAGETLHFFEEVYEHGKIPEDDSIRRIYMARHIMEKFIVAGAEMELNLSHKTRQEILTTQDLTHTDLFKNALNEVMQLIKMNLVRDYWSSIYFIKFKEEESCHEAMHKEGYSFSSPRLSSVQGSDDPFYQEHMSKSSRCSSPG

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1I9LSX6A0A1I9LSX6_ARATHRGS1399

Sequence caution

The sequence BAB01073.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence BAB01074.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict426in Ref. 3; AAM14091

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB023041
EMBL· GenBank· DDBJ
BAB01073.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AB023041
EMBL· GenBank· DDBJ
BAB01074.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002686
EMBL· GenBank· DDBJ
AEE77116.1
EMBL· GenBank· DDBJ
Genomic DNA
AY091142
EMBL· GenBank· DDBJ
AAM14091.1
EMBL· GenBank· DDBJ
mRNA
AY150429
EMBL· GenBank· DDBJ
AAN12971.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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