Q8H1F2 · RGS1_ARATH
- ProteinRegulator of G-protein signaling 1
- GeneRGS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids459 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway (PubMed:26528314).
Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K+ and anion channels. Important for the kinetics of voltage activation of inward K+ current but not for the current amplitude.
Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K+ and anion channels. Important for the kinetics of voltage activation of inward K+ current but not for the current amplitude.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endosome membrane | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | G protein-coupled receptor activity | |
Molecular Function | GTPase activator activity | |
Biological Process | jasmonic acid mediated signaling pathway | |
Biological Process | negative regulation of signal transduction | |
Biological Process | positive regulation of GTPase activity | |
Biological Process | regulation of cell population proliferation | |
Biological Process | response to abscisic acid | |
Biological Process | response to glucose | |
Biological Process | response to water deprivation | |
Biological Process | sugar mediated signaling pathway |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameRegulator of G-protein signaling 1
- Short namesAtRGS1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8H1F2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Endosome membrane ; Multi-pass membrane protein
Note: accumulates at the nascent cell plate during cytokinesis.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 9-29 | Helical | ||||
Sequence: GGCPSDYVAVAISVICFFVLL | ||||||
Transmembrane | 48-68 | Helical | ||||
Sequence: SFWIPVIQVISSFNLLFSIMM | ||||||
Transmembrane | 83-103 | Helical | ||||
Sequence: YCYLWAVWIEGPLGFGLLMSC | ||||||
Transmembrane | 126-146 | Helical | ||||
Sequence: YIFLPLVLLPWIFGAAIIHAT | ||||||
Transmembrane | 157-177 | Helical | ||||
Sequence: LQWTFPVAGLHALYVLALIAF | ||||||
Transmembrane | 194-214 | Helical | ||||
Sequence: LWKGILVSATSIVIWVTAFVL | ||||||
Transmembrane | 226-246 | Helical | ||||
Sequence: VASRFVLLVTGGILVVVFFSI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Increased cell elongation and longer hypocotyls when grown in the dark. Longer primary roots when grown in light due to increased cell production in root meristem. Loss of stratification requirement for seed germination. Decreased sensitivity to glucose and abscisic acid (ABA) in seed germination process (PubMed:26528314).
No effect on ABA inhibition of stomatal opening
No effect on ABA inhibition of stomatal opening
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 320 | Loss of GTPase-accelerating activity, loss of interaction with GPA1 and loss of endocytosis. | ||||
Sequence: E → K | ||||||
Mutagenesis | 428 | Loss of endocytosis; when associated with A-435 and A-436. | ||||
Sequence: S → A | ||||||
Mutagenesis | 435 | Loss of endocytosis; when associated with A-428 and A-436. | ||||
Sequence: S → A | ||||||
Mutagenesis | 436 | Loss of endocytosis; when associated with A-428 and A-435. | ||||
Sequence: S → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 42 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000421264 | 1-459 | Regulator of G-protein signaling 1 | |||
Sequence: MASGCALHGGCPSDYVAVAISVICFFVLLSRSVLPCLIHKAPRTNSSSFWIPVIQVISSFNLLFSIMMSVNLLRFRTKHWWRYCYLWAVWIEGPLGFGLLMSCRITQAFQLYFIFVKKRLPPVKSYIFLPLVLLPWIFGAAIIHATKPLNDKCHMGLQWTFPVAGLHALYVLALIAFTRAVRHVEFRFDELRDLWKGILVSATSIVIWVTAFVLNEIHEEISWLQVASRFVLLVTGGILVVVFFSISSNQPLLSQISLKKRQNFEFQRMGQALGIPDSGLLFRKEEFRPVDPNEPLDKLLLNKRFRHSFMEFADSCYAGETLHFFEEVYEHGKIPEDDSIRRIYMARHIMEKFIVAGAEMELNLSHKTRQEILTTQDLTHTDLFKNALNEVMQLIKMNLVRDYWSSIYFIKFKEEESCHEAMHKEGYSFSSPRLSSVQGSDDPFYQEHMSKSSRCSSPG | ||||||
Modified residue | 428 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by WNK8 and WNK10, and in vitro by WNK1. Also phosphorylated at Ser-435 or Ser-436.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in hypocotyls, cotyledons, guard cells and root and shoot apical meristems.
Induction
Up-regulated by ozone.
Gene expression databases
Interaction
Subunit
Interacts (via C-ter) with GPA1 (PubMed:14500984, PubMed:17951432, PubMed:18817773, PubMed:21952135).
Interacts with WNK1, WNK8, WNK10, SYP23. The association with WNK8 at the plasma membrane is triggered by induction of glucose and continues with trafficking into endocytic compartments (PubMed:21952135).
Interacts with NDL1, NDL2 and NDL3 (PubMed:19948787).
Interacts with RHIP1 (PubMed:26528314).
Interacts with WNK1, WNK8, WNK10, SYP23. The association with WNK8 at the plasma membrane is triggered by induction of glucose and continues with trafficking into endocytic compartments (PubMed:21952135).
Interacts with NDL1, NDL2 and NDL3 (PubMed:19948787).
Interacts with RHIP1 (PubMed:26528314).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8H1F2 | GPA1 P18064 | 7 | EBI-1627025, EBI-443890 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 295-413 | RGS | ||||
Sequence: PLDKLLLNKRFRHSFMEFADSCYAGETLHFFEEVYEHGKIPEDDSIRRIYMARHIMEKFIVAGAEMELNLSHKTRQEILTTQDLTHTDLFKNALNEVMQLIKMNLVRDYWSSIYFIKFK | ||||||
Region | 429-459 | Disordered | ||||
Sequence: FSSPRLSSVQGSDDPFYQEHMSKSSRCSSPG |
Domain
The C-terminal domain (249-459) is able to bind to and to accelerate the GTPase activity of GPA1.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length459
- Mass (Da)52,947
- Last updated2003-03-01 v1
- Checksum43AA65CD0417488E
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LSX6 | A0A1I9LSX6_ARATH | RGS1 | 399 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 426 | in Ref. 3; AAM14091 | ||||
Sequence: G → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB023041 EMBL· GenBank· DDBJ | BAB01073.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AB023041 EMBL· GenBank· DDBJ | BAB01074.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE77116.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY091142 EMBL· GenBank· DDBJ | AAM14091.1 EMBL· GenBank· DDBJ | mRNA | ||
AY150429 EMBL· GenBank· DDBJ | AAN12971.1 EMBL· GenBank· DDBJ | mRNA |