Q8H0U8 · RH42_ARATH
- ProteinDEAD-box ATP-dependent RNA helicase 42
- GeneRH42
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1166 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Helicase required for pre-mRNA splicing, cold-responsive gene regulation and cold tolerance.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 573-580 | ATP (UniProtKB | ChEBI) | |||
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDEAD-box ATP-dependent RNA helicase 42
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8H0U8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 687 | Loss of ATPase activity. | |||
Mutagenesis | 808 | In rcf1-1; loss of function and reduced cold tolerance. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 46 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000239182 | 1-1166 | DEAD-box ATP-dependent RNA helicase 42 | ||
Modified residue | 210 | Phosphoserine | |||
Modified residue | 324 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Family & Domains
Features
Showing features for compositional bias, region, coiled coil, motif, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-45 | Basic and acidic residues | |||
Region | 1-460 | Disordered | |||
Coiled coil | 14-95 | ||||
Compositional bias | 68-83 | Basic and acidic residues | |||
Compositional bias | 92-311 | Basic and acidic residues | |||
Coiled coil | 130-302 | ||||
Compositional bias | 325-346 | Acidic residues | |||
Compositional bias | 416-430 | Basic and acidic residues | |||
Motif | 529-557 | Q motif | |||
Domain | 560-738 | Helicase ATP-binding | |||
Motif | 686-689 | DEAD box | |||
Domain | 749-910 | Helicase C-terminal | |||
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8H0U8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,166
- Mass (Da)133,033
- Last updated2006-05-30 v2
- Checksum597BD171F02666B5
Q8H0U8-2
- Name2
- Differences from canonical
- 1-338: Missing
Sequence caution
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-45 | Basic and acidic residues | |||
Alternative sequence | VSP_053521 | 1-338 | in isoform 2 | ||
Compositional bias | 68-83 | Basic and acidic residues | |||
Compositional bias | 92-311 | Basic and acidic residues | |||
Compositional bias | 325-346 | Acidic residues | |||
Compositional bias | 416-430 | Basic and acidic residues | |||
Sequence conflict | 894 | in Ref. 3; AAN72041 | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC007369 EMBL· GenBank· DDBJ | AAD30599.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE30040.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE30041.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM59668.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM59669.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM59670.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM59671.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM59672.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT002030 EMBL· GenBank· DDBJ | AAN72041.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |