Q8GYQ5 · MPK12_ARATH

Function

function

Negative regulator of the auxin transduction signaling pathway (PubMed:19000167).
Involved in stomatal movement regulation by phosphorylating and repressing HT1 and HT1-mediated GHR1 phosphorylation (PubMed:27694184, PubMed:27923039).
Required for CO2-mediated stomatal movements (e.g. closure) (PubMed:27923039).

Catalytic activity

Activity regulation

Activated by threonine and tyrosine phosphorylation.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site47-55ATP (UniProtKB | ChEBI)
Site53Involved in HT1 binding
Binding site70ATP (UniProtKB | ChEBI)
Active site167Proton acceptor

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionkinase activity
Molecular FunctionMAP kinase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein tyrosine kinase activity
Biological Processauxin-activated signaling pathway
Biological Processcellular response to carbon dioxide
Biological Processintracellular signal transduction
Biological Processprotein autophosphorylation
Biological Processregulation of stomatal closure
Biological Processregulation of stomatal movement
Biological Processresponse to auxin
Biological Processresponse to indolebutyric acid

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Mitogen-activated protein kinase 12
  • EC number
  • Short names
    AtMPK12
    ; MAP kinase 12

Gene names

    • Name
      MPK12
    • ORF names
      T3F17.28
    • Ordered locus names
      At2g46070

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8GYQ5
  • Secondary accessions
    • O82361

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus
Cell membrane
; Peripheral membrane protein
Note: Seems to translocate at the plasma membrane upon interaction with HT1.

Keywords

Phenotypes & Variants

Disruption phenotype

Lethal in homozygous plants (PubMed:27923039).
Abolished CO2-mediated stomatal closure (PubMed:27694184, PubMed:27923039).
Increased stomatal opening (PubMed:27923039).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis53Increased stomatal opening and altered CO2 and ozone (O3) responses. Weaker interaction with HT1. Strongly suppressed inhibition of HT1 activity. Loss of autophosphorylation.
Mutagenesis70Loss of kinase activity. Reduced inhibition of HT1 activity. Loss of autophosphorylation.
Mutagenesis122Hyperactive kinase, can inhibit HT1 activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002458121-372Mitogen-activated protein kinase 12
Modified residue199Phosphothreonine
Modified residue201Phosphotyrosine
Modified residue204Phosphothreonine

Post-translational modification

Autophosphorylated (PubMed:27923039).
Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme (By similarity).
Activated by auxin. Dephosphorylated and inactivated by IBR5

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.

Gene expression databases

Interaction

Subunit

Interacts with IBR5/SKIP33 (PubMed:19000167, PubMed:27923039).
Binds to HT1 (PubMed:27694184, PubMed:27923039).

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif, region.

TypeIDPosition(s)Description
Domain41-327Protein kinase
Motif199-201TXY
Region210-372IBR5 binding

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q8GYQ5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    372
  • Mass (Da)
    42,475
  • Last updated
    2003-03-01 v1
  • Checksum
    1A8FF099D1DC80B9
MSGESSSGSTEHCIKVVPTHGGRYVQYNVYGQLFEVSRKYVPPIRPIGRGACGIVCAAVNSVTGEKVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHENVITIKDIVRPPQRDIFNDVYIVYELMDTDLQRILRSNQTLTSDQCRFLVYQLLRGLKYVHSANILHRDLRPSNVLLNSKNELKIGDFGLARTTSDTDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTGQPLFPGKDYVHQLRLITELVGSPDNSSLGFLRSDNARRYVRQLPRYPKQQFAARFPKMPTTAIDLLERMLVFDPNRRISVDEALGHAYLSPHHDVAKEPVCSTPFSFDFEHPSCTEEHIKELIYKESVKFNPDH

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F4IH84F4IH84_ARATHMPK12324
A0A178VX75A0A178VX75_ARATHMPK12406

Sequence caution

The sequence AAC62906.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC005397
EMBL· GenBank· DDBJ
AAC62906.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
CP002685
EMBL· GenBank· DDBJ
AEC10639.1
EMBL· GenBank· DDBJ
Genomic DNA
AK117449
EMBL· GenBank· DDBJ
BAC42114.1
EMBL· GenBank· DDBJ
mRNA
BT024898
EMBL· GenBank· DDBJ
ABD85169.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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