Q8GV50 · PLA2D_ARATH
- ProteinPhospholipase A2-delta
- GenePLA2-DELTA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids191 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli. Plays a role in pollen development and germination and tube growth.
Miscellaneous
The enzyme has a slight preference for phosphatidylethanolamine over phosphatidylcholine.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+
Cofactor
Note: Binds 1 Ca2+ ion per subunit.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | extracellular region | |
Molecular Function | calcium ion binding | |
Molecular Function | phospholipase A2 activity | |
Biological Process | arachidonic acid secretion | |
Biological Process | lipid catabolic process | |
Biological Process | phospholipid metabolic process | |
Biological Process | pollen development | |
Biological Process | pollen germination | |
Biological Process | pollen tube growth |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhospholipase A2-delta
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8GV50
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-25 | |||||
Sequence: MIRGGALTHVALGLTVFLLLAVVHS | ||||||
Chain | PRO_0000417564 | 26-191 | Phospholipase A2-delta | |||
Sequence: QEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPPCDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVIPTVYRGMNYGIFFSGIGNIIIPKKPASAGPVVEVDLARSKADTKDGLGTNQGPQTKDGSKVSVPMNPSPS | ||||||
Disulfide bond | 29↔56 | |||||
Sequence: CSKTCIAQKCNVLGIRYGKYCGIGYFGC | ||||||
Disulfide bond | 33↔62 | |||||
Sequence: CIAQKCNVLGIRYGKYCGIGYFGCPGEPPC | ||||||
Disulfide bond | 38↔115 | |||||
Sequence: CNVLGIRYGKYCGIGYFGCPGEPPCDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKEC | ||||||
Disulfide bond | 49↔69 | |||||
Sequence: CGIGYFGCPGEPPCDDLDDCC | ||||||
Disulfide bond | 68↔93 | |||||
Sequence: CCMTHDNCVDLKGMTYVDCHKQFQRC | ||||||
Disulfide bond | 75↔86 | |||||
Sequence: CVDLKGMTYVDC |
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Specifically expressed in flowers but at a low level. Detected specifically in the pollen.
Developmental stage
Expressed during pollen germination and tube growth.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 161-191 | Disordered | ||||
Sequence: KADTKDGLGTNQGPQTKDGSKVSVPMNPSPS | ||||||
Compositional bias | 165-191 | Polar residues | ||||
Sequence: KDGLGTNQGPQTKDGSKVSVPMNPSPS |
Sequence similarities
Belongs to the phospholipase A2 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length191
- Mass (Da)20,617
- Last updated2003-03-01 v1
- Checksum3E6F729E288CB149
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 165-191 | Polar residues | ||||
Sequence: KDGLGTNQGPQTKDGSKVSVPMNPSPS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY148347 EMBL· GenBank· DDBJ | AAN63045.1 EMBL· GenBank· DDBJ | mRNA | ||
AL161575 EMBL· GenBank· DDBJ | CAB79705.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE85635.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | ANM66586.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB493706 EMBL· GenBank· DDBJ | BAH30544.1 EMBL· GenBank· DDBJ | mRNA |