Q8GS71 · KN4A_ARATH

Function

function

Kinesin-like motor protein involved in the control of the oriented deposition of cellulose microfibrils (PubMed:12468730, Ref.6). Its motor activity is directed toward the microtubule's plus end. It possesses the potential to drive long-distance transport of cargo along cortical microtubules (PubMed:21914648, PubMed:25646318).
Regulates cell wall mechanics during cell elongation, by the regulation of primary and secondary walls deposition (PubMed:25600279, PubMed:25646318, Ref.6). Contributes to cortical microtubule-mediated trafficking of cell wall components (PubMed:25646318).

Miscellaneous

Overexpression of KIN4A/FRA1 caused a severe reduction in the thickness of secondary walls in interfascicular fibers and deformation of vessels, an increase in the number of secondary wall layers, which are accompanied with a marked decrease in stem strength.

Features

Showing features for binding site.

110351002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site90-97ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcortical microtubule
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular Functionmicrotubule binding
Molecular Functionmicrotubule motor activity
Molecular Functionplus-end-directed microtubule motor activity
Molecular Functionsequence-specific DNA binding
Biological Processcellulose microfibril organization
Biological Processcytoskeleton-dependent intracellular transport
Biological Processmicrotubule-based movement
Biological Processplant-type cell wall biogenesis
Biological Processpositive regulation of DNA-templated transcription
Biological Processregulation of cell population proliferation
Biological Processregulation of gibberellic acid mediated signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Kinesin-like protein KIN-4A
  • Alternative names
    • AtKINESIN-4A
    • Protein FRAGILE FIBER 1

Gene names

    • Name
      KIN4A
    • Synonyms
      FRA1
    • ORF names
      MCA23.16
    • Ordered locus names
      At5g47820

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8GS71
  • Secondary accessions
    • Q9FIJ9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cytoplasm
Note: Concentrated around the periphery of the cytoplasm.

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced length of roots and inflorescence stems. Altered orientation of cellulose microfibrils in fiber walls leading to dramatic reduction in fiber mechanical strength. No apparent alteration in cell wall composition. Lower expansion rate of the inflorescence stem along with the reduced thickness of both primary and secondary cell walls leading to mechanically weaker stems (PubMed:25646318).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 53 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004319621-1035Kinesin-like protein KIN-4A

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in stems and flowers (PubMed:12468730).
Detected in cells undergoing secondary wall deposition including developing interfascicular fibers and xylem cells, but also in dividing cells and expanding/elongating parenchyma cells (Ref.6)

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, coiled coil, region, compositional bias.

TypeIDPosition(s)Description
Domain11-370Kinesin motor
Coiled coil408-436
Coiled coil504-707
Region704-724Disordered
Compositional bias710-724Polar residues
Coiled coil881-911

Domain

Composed of an N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule) and a central to C-terminal alpha-helical coiled coil domain that mediates the heavy chain dimerization.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,035
  • Mass (Da)
    116,734
  • Last updated
    2003-03-01 v1
  • Checksum
    6DF0B4C16B88588C
MESTPPPDDCSVKVAVHIRPLIGDERIQGCQDCVTVVTGKPQVQIGSHSFTFDHVYGSSGSPSTEMYEECAAPLVDGLFQGYNATVLAYGQTGSGKTYTMGTGCGDSSQTGIIPQVMNALFTKIETLKQQIEFQIHVSFIEIHKEEVQDLLDPCTVNKSDTNNTGHVGKVAHVPGKPPIQIRETSNGVITLAGSTEVSVSTLKEMAACLDQGSVSRATGSTNMNNQSSRSHAIFTITVEQMRKINTDSPENGAYNGSLKEEYLCAKLHLVDLAGSERAKRTGSDGLRFKEGVHINKGLLALGNVISALGDEKKRKDGAHVPYRDSKLTRLLQDSLGGNSRTVMIACISPADINAEETLNTLKYANRARNIRNKPVVNRDPVSSEMLKMRQQVEYLQAELSLRTGGSSCAEVQALKERIVWLETANEELCRELHEYRSRCPGVEHSEKDFKDIRADDIVGSVRPDGLKRSLHSIESSNYPMVEATTGDSREIDEEAKEWEHKLLQNSMDKELYELNRRLEEKESEMKLFDGYDPAALKQHFGKKIAEVEDEKRSVQEERNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQILDLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQKVQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEAAMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEVRHEYEKQSHVRAALAEELAVLRQVDEFAVKGLSPPRGKNGFARASSLSPNARMARISSLENMLVISSNSLVAMASQLSEAEERERAFTNRGRWNQLRSMGEAKNLLQYMFNSLAETRCQLWEKDVEIKEMKDQFKEIVGLLRQSELRRKEAEKELKLREQAIATSLGTPPSSVKHVAEDLSTPSPMTVPAQKQLKFTPGIANGKVRGPAAFLDTNKKMVPMGQVSMRKLSAVGKQGGRLWRWKRSHHQWIVQFKWKWQKPWRLSEWIRTSDETLLKSKPRLKALPNKIM

Sequence caution

The sequence BAB11329.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias710-724Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY158083
EMBL· GenBank· DDBJ
AAN86114.1
EMBL· GenBank· DDBJ
mRNA
AY158084
EMBL· GenBank· DDBJ
AAN86115.1
EMBL· GenBank· DDBJ
Genomic DNA
AB016886
EMBL· GenBank· DDBJ
BAB11329.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002688
EMBL· GenBank· DDBJ
AED95575.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED95576.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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