Q8GEA9 · BGAL1_THESP
- ProteinBeta-galactosidase BgaA
- GenebgaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids645 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and p-nitrophenyl-beta-D-galactoside (pNPGal).
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.41 mM | pNPGal | 7.0 | 70 | |||
42 mM | lactose | 7.0 | 70 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
140 μmol/min/mg | 7.0 | 70 | with pNPGal as substrate | ||
8.5 μmol/min/mg | 7.0 | 70 | with lactose as substrate |
pH Dependence
Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.
Temperature Dependence
Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 102 | substrate | |||
Binding site | 106 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 140 | substrate | |||
Active site | 141 | Proton donor | |||
Binding site | 150 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 152 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 155 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 312 | Nucleophile | |||
Binding site | 320 | substrate | |||
Binding site | 360-363 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | beta-galactosidase complex | |
Molecular Function | beta-galactosidase activity | |
Molecular Function | metal ion binding | |
Biological Process | galactose metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-galactosidase BgaA
- EC number
- Short namesBeta-gal
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Deinococcota > Deinococci > Thermales > Thermaceae > Thermus
Accessions
- Primary accessionQ8GEA9
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000407695 | 1-645 | Beta-galactosidase BgaA | ||
Structure
Sequence
- Sequence statusComplete
- Length645
- Mass (Da)72,714
- Last updated2003-03-01 v1
- ChecksumE1F13D0D598857BE
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY130259 EMBL· GenBank· DDBJ | AAN05443.1 EMBL· GenBank· DDBJ | Genomic DNA |