Q8GEA9 · BGAL1_THESP

  • Protein
    Beta-galactosidase BgaA
  • Gene
    bgaA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Organism
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and p-nitrophenyl-beta-D-galactoside (pNPGal).

Catalytic activity

  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
    EC:3.2.1.23 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.41 mMpNPGal7.070
42 mMlactose7.070
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
140 μmol/min/mg7.070with pNPGal as substrate
8.5 μmol/min/mg7.070with lactose as substrate

pH Dependence

Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.

Temperature Dependence

Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site102substrate
Binding site106Zn2+ (UniProtKB | ChEBI)
Binding site140substrate
Active site141Proton donor
Binding site150Zn2+ (UniProtKB | ChEBI)
Binding site152Zn2+ (UniProtKB | ChEBI)
Binding site155Zn2+ (UniProtKB | ChEBI)
Active site312Nucleophile
Binding site320substrate
Binding site360-363substrate

GO annotations

AspectTerm
Cellular Componentbeta-galactosidase complex
Molecular Functionbeta-galactosidase activity
Molecular Functionmetal ion binding
Biological Processgalactose metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-galactosidase BgaA
  • EC number
  • Short names
    Beta-gal

Gene names

    • Name
      bgaA

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ATCC 43815 / IB-21
  • Taxonomic lineage
    Bacteria > Deinococcota > Deinococci > Thermales > Thermaceae > Thermus

Accessions

  • Primary accession
    Q8GEA9

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004076951-645Beta-galactosidase BgaA

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    645
  • Mass (Da)
    72,714
  • Last updated
    2003-03-01 v1
  • Checksum
    E1F13D0D598857BE
MLGVCYYPEHWPKARWKEDARRMREAGLSYVRVGEFAWALLEPEPGRLEWGWLDEALATLAAEGLKVVLGTPTATPPKWLVDRYPEVLPVDREGRRRRFGGRRHYCFSSPAYREEARRIVTLLAERYGGLEAVAGFQTDNEYGCHGTVRCYCPRCQEAFRGWLKARYGTIEALNEAWGTAFWSQRYRNFTEVELPHLTVAEPNPSHLLDYYRFASDQVRAFNRLQVEILRAHAPGKFITHNFMGFFTDLDAFALAQDLDFASWDSYPLGFTDLMPLPPEEKLRYARTGHPDVAAFHHDLYRGVGRGRFWVMEQQPGPVNWAPHNPSPTPGMVRLWTWEALAHGAEVVSYFRWRQAPFAQEQMHAGLHRPDSAPDQGFFEAKQVAEELAALALPPVAQAPVALVFDYEAAWVYEVQPQGAEWSYLGLIYLFYSALRRLGLDVDVVPPGASLRGYALTVVPSLPIVRGEALKAFQEAEGIVLFGPRSGSKTETFQIPRELPPGPLQALLPLKVVRVESLPPGLLEVAEGPMGRFSLGLWREWVESPLRPWLAFADGGGALYREGRYLYLAAWPSPELLGVLLAGLAQEAGLRPVFLPEGLRLRRRGPWVFAFNYGPEAVEAPAPEGARFLLGGKRVGPYDLAVWEEA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY130259
EMBL· GenBank· DDBJ
AAN05443.1
EMBL· GenBank· DDBJ
Genomic DNA

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