Q8GAW0 · CPMO_COMS9
- ProteinCyclopentanone 1,2-monooxygenase
- GenecpnB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids550 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters or lactones using NADPH as an electron donor. Converts cyclopentanone to 5-valerolactone, a step in the degradation pathway of cyclopentanol. Besides cycloalkanones, can also act on methylated and other alkylated cycloalkanones, and on methylated cycloalkenones, with high enantioselectivity in some cases. Cannot use NADH instead of NADPH.
Catalytic activity
- cyclopentanone + H+ + NADPH + O2 = 5-valerolactone + H2O + NADP+
Cofactor
Pathway
Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 2/2.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 31-32 | FAD (UniProtKB | ChEBI) | ||||
Sequence: FT | ||||||
Binding site | 51 | FAD (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 60 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 71 | FAD (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 77 | FAD (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 123 | FAD (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Site | 344 | Transition state stabilizer | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | cyclopentanone monooxygenase activity | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | N,N-dimethylaniline monooxygenase activity | |
Molecular Function | NADP binding | |
Biological Process | cyclopentanol catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCyclopentanone 1,2-monooxygenase
- EC number
- Short namesCPMO
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Comamonas
Accessions
- Primary accessionQ8GAW0
- Secondary accessions
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene are not capable of growth on cyclopentanol or cyclopentanone as a sole carbon and energy source.
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000186453 | 2-550 | Cyclopentanone 1,2-monooxygenase | |||
Sequence: TTMTTMTTEQLGMNNSVNDKLDVLLIGAGFTGLYQLYHLRKLGYKVHLVDAGADIGGIWHWNCYPGARVDTHCQIYQYSIPELWQEFNWKELFPNWAQMREYFHFADKKLDLSKDISFNTRVQSAVFDEGTREWTVRSIGHQPIQARFVIANLGFGASPSTPNVDGIETFKGQWYHTALWPQEGVNMAGKRVAIIGTGSSGVQVAQEAALDAKQVTVYQRTPNLALPMHQKQLSAEDNLRMKPELPAAFERRGKCFAGFDFDFIAKNATELSAAERTEILEELWNAGGFRYWLANFQDYLFDDKANDYVYEFWRDKVRARIKDPKVAEKLAPMKKPHPYGAKRPSLEQWYYEIFNQNNVTLVDVNETPVLRITEKGIVTAEGEAEFDLIVFATGFDAVTGGLTSIDFRNNQGQSFKDVWSDGIRTQLGVATAGFPNLLFGYGPQSPAGFCNGPSSAEYQGDLLIQLMNYLRDNNISRIEAQSEAQEEWSKLIADFWDSSLFPRAKSWYQGSNIPGKKVESLNFPLGLPTYISKFNESAEKGYAGFSLAS |
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length550
- Mass (Da)62,111
- Last updated2007-01-23 v3
- Checksum8956EF95203F6173
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB073151 EMBL· GenBank· DDBJ | BAC22652.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB022102 EMBL· GenBank· DDBJ | BAC01269.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AJ418060 EMBL· GenBank· DDBJ | CAD10798.1 EMBL· GenBank· DDBJ | Genomic DNA |