Q8GAW0 · CPMO_COMS9

Function

function

Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters or lactones using NADPH as an electron donor. Converts cyclopentanone to 5-valerolactone, a step in the degradation pathway of cyclopentanol. Besides cycloalkanones, can also act on methylated and other alkylated cycloalkanones, and on methylated cycloalkenones, with high enantioselectivity in some cases. Cannot use NADH instead of NADPH.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 2/2.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site31-32FAD (UniProtKB | ChEBI)
Binding site51FAD (UniProtKB | ChEBI)
Binding site60FAD (UniProtKB | ChEBI)
Binding site71FAD (UniProtKB | ChEBI)
Binding site77FAD (UniProtKB | ChEBI)
Binding site123FAD (UniProtKB | ChEBI)
Site344Transition state stabilizer

GO annotations

AspectTerm
Molecular Functioncyclopentanone monooxygenase activity
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionN,N-dimethylaniline monooxygenase activity
Molecular FunctionNADP binding
Biological Processcyclopentanol catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cyclopentanone 1,2-monooxygenase
  • EC number
  • Short names
    CPMO
  • Alternative names
    • Baeyer-Villiger monooxygenase (BVMO)

Gene names

    • Name
      cpnB
    • Synonyms
      cpmA

Organism names

Accessions

  • Primary accession
    Q8GAW0
  • Secondary accessions
    • Q937L5

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene are not capable of growth on cyclopentanol or cyclopentanone as a sole carbon and energy source.

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001864532-550Cyclopentanone 1,2-monooxygenase

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Sequence similarities

Belongs to the FAD-binding monooxygenase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    550
  • Mass (Da)
    62,111
  • Last updated
    2007-01-23 v3
  • Checksum
    8956EF95203F6173
MTTMTTMTTEQLGMNNSVNDKLDVLLIGAGFTGLYQLYHLRKLGYKVHLVDAGADIGGIWHWNCYPGARVDTHCQIYQYSIPELWQEFNWKELFPNWAQMREYFHFADKKLDLSKDISFNTRVQSAVFDEGTREWTVRSIGHQPIQARFVIANLGFGASPSTPNVDGIETFKGQWYHTALWPQEGVNMAGKRVAIIGTGSSGVQVAQEAALDAKQVTVYQRTPNLALPMHQKQLSAEDNLRMKPELPAAFERRGKCFAGFDFDFIAKNATELSAAERTEILEELWNAGGFRYWLANFQDYLFDDKANDYVYEFWRDKVRARIKDPKVAEKLAPMKKPHPYGAKRPSLEQWYYEIFNQNNVTLVDVNETPVLRITEKGIVTAEGEAEFDLIVFATGFDAVTGGLTSIDFRNNQGQSFKDVWSDGIRTQLGVATAGFPNLLFGYGPQSPAGFCNGPSSAEYQGDLLIQLMNYLRDNNISRIEAQSEAQEEWSKLIADFWDSSLFPRAKSWYQGSNIPGKKVESLNFPLGLPTYISKFNESAEKGYAGFSLAS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB073151
EMBL· GenBank· DDBJ
BAC22652.1
EMBL· GenBank· DDBJ
Genomic DNA
AB022102
EMBL· GenBank· DDBJ
BAC01269.1
EMBL· GenBank· DDBJ
Genomic DNA
AJ418060
EMBL· GenBank· DDBJ
CAD10798.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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