Q8G8B6 · CARAA_PSERE
- ProteinCarbazole 1,9a-dioxygenase, terminal oxygenase component CarAa
- GenecarAa
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids384 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Part of the multicomponent carbazole 1,9a-dioxygenase (CARDO), that converts carbazole (CAR) into 2-aminobiphenyl-2,3-diol. Catalyzes the dioxygenation at the angular (C-9a) and adjacent (C-1) positions of carbazole to yield a highly unstable cis-hydrodiol intermediate which is spontaneously converted to 2-aminobiphenyl-2,3-diol. It is also able to attack the angular position adjacent of hetero atom of heterocyclic aromatic compounds such as polychlorinated dibenzo-p-dioxin (DD) and dibenzofuran (DBF). It was also shown that CARDO has the ability to metabolize biphenyl and polycyclic aromatic hydrocarbons, such as naphthalene and phenanthrene.
Catalytic activity
- 9H-carbazole + H+ + NADH + O2 = 2'-aminobiphenyl-2,3-diol + NAD+
Cofactor
Note: Binds 1 [2Fe-2S] cluster per subunit.
pH Dependence
Optimum pH is between 7 and 7.5.
Temperature Dependence
Optimum temperature is 30 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | carbazole 1,9a-dioxygenase activity | |
Molecular Function | metal ion binding | |
Biological Process | carbazole catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbazole 1,9a-dioxygenase, terminal oxygenase component CarAa
- EC number
- Short namesCARDO
Gene names
Encoded on
- Plasmid pCAR1
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ8G8B6
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000419024 | 2-384 | Carbazole 1,9a-dioxygenase, terminal oxygenase component CarAa | |||
Sequence: ANVDEAILKRVKGWAPYVDAKLGFRNHWYPVMFSKEIDEGEPKTLKLLGENLLVNRIDGKLYCLKDRCLHRGVQLSVKVECKTKSTITCWYHAWTYRWEDGVLCDILTNPTSAQIGRQKLKTYPVQEAKGCVFIYLGDGDPPPLARDTPPNFLDDDMEILGKNQIIKSNWRLAVENGFDPSHIYIHKDSILVKDNDLALPLGFAPGGDRKQQTRVVDDDVVGRKGVYDLIGEHGVPVFEGTIGGEVVREGAYGEKIVANDISIWLPGVLKVNPFPNPDMMQFEWYVPIDENTHYYFQTLGKPCANDEERKNYEQEFESKWKPMALEGFNNDDIWAREAMVDFYADDKGWVNEILFEVDEAIVAWRKLASEHNQGIQTQAHVSG |
Interaction
Subunit
Homotrimer. Carbazole 1,9a-dioxygenase complex consists of a terminal oxygenase component CarAa, a ferredoxin reductase component CarAd and a ferredoxin component CarAc.
Structure
Sequence
- Sequence statusComplete
- Length384
- Mass (Da)43,785
- Last updated2003-03-01 v1
- ChecksumE941848321A6A728
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB047548 EMBL· GenBank· DDBJ | BAB32765.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB047548 EMBL· GenBank· DDBJ | BAB32766.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB088420 EMBL· GenBank· DDBJ | BAC41544.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB088420 EMBL· GenBank· DDBJ | BAC41545.1 EMBL· GenBank· DDBJ | Genomic DNA |