Q8FDI4 · ASST_ECOL6
- ProteinArylsulfate sulfotransferase AssT
- GeneassT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids598 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the transfer of a sulfate group from a phenyl sulfate ester to other phenolic compounds. In vitro, is able to use 4-methylumbelliferyl sulfate and p-nitrophenyl sulfate (PNS) as donor substrates with phenol as the acceptor substrate (PubMed:18565543, PubMed:19036922).
Cannot use 3'-phosphoadenosine-5'-phophosulfate (PAPS), the donor substrate of mammalian sulfotransferase (PubMed:19036922).
Cannot use 3'-phosphoadenosine-5'-phophosulfate (PAPS), the donor substrate of mammalian sulfotransferase (PubMed:19036922).
Miscellaneous
Most commensal E.coli strains do not have a gene for arylsulfate sulfotransferase, in contrast to strain CFT073 and other uropathogenic strains.
Catalytic activity
- an aryl sulfate + a phenol = an aryl sulfate + a phenol
- 4-methylumbelliferone sulfate + phenol = phenyl sulfate + 4-methylumbelliferone
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1.35 mM | phenol | 8.0 | ||||
45 μM | 4-methylumbelliferylsulfate | 8.0 |
kcat is 48.6 sec-1 with phenol and 4-methylumbelliferylsulfate as substrates (at pH 8.0).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 279 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 383 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 463 | Nucleophile; sulfurylated histidine covalent intermediate | ||||
Sequence: H | ||||||
Binding site | 463 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | aryl sulfotransferase activity | |
Molecular Function | arylsulfate sulfotransferase activity | |
Molecular Function | identical protein binding |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameArylsulfate sulfotransferase AssT
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ8FDI4
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 123 | No effect. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 235 | No effect. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 279 | Reduces enzyme activity by 96%. | ||||
Sequence: H → L | ||||||
Mutagenesis | 383 | Reduces enzyme activity by over 99%. | ||||
Sequence: H → L | ||||||
Mutagenesis | 401 | Reduces enzyme activity by over 99%. | ||||
Sequence: R → L | ||||||
Mutagenesis | 586 | Reduces enzyme activity by about 40%. | ||||
Sequence: Y → F |
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MFDKYRKTLVAGTVAITLGLSASGVMA | ||||||
Chain | PRO_0000418844 | 28-598 | Arylsulfate sulfotransferase AssT | |||
Sequence: AGFKPAPPAGQLGAVIVDPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTHTFTAQGXDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK | ||||||
Disulfide bond | 445↔451 | |||||
Sequence: CNENGLC |
Post-translational modification
The disulfide bond is crucial for enzyme activity.
Keywords
- PTM
Expression
Induction
Expressed from a tri-cistronic operon that encodes AssT, DsbI and DsbL.
Interaction
Subunit
Homodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8FDI4 | assT Q8FDI4 | 2 | EBI-15743267, EBI-15743267 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length598
- Mass (Da)66,570
- Last updated2003-03-01 v1
- Checksum309EE9E1580D7FB0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014075 EMBL· GenBank· DDBJ | AAN82229.1 EMBL· GenBank· DDBJ | Genomic DNA |