Q8FDI4 · ASST_ECOL6

Function

function

Catalyzes the transfer of a sulfate group from a phenyl sulfate ester to other phenolic compounds. In vitro, is able to use 4-methylumbelliferyl sulfate and p-nitrophenyl sulfate (PNS) as donor substrates with phenol as the acceptor substrate (PubMed:18565543, PubMed:19036922).
Cannot use 3'-phosphoadenosine-5'-phophosulfate (PAPS), the donor substrate of mammalian sulfotransferase (PubMed:19036922).

Miscellaneous

Most commensal E.coli strains do not have a gene for arylsulfate sulfotransferase, in contrast to strain CFT073 and other uropathogenic strains.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.35 mMphenol8.0
45 μM4-methylumbelliferylsulfate8.0
kcat is 48.6 sec-1 with phenol and 4-methylumbelliferylsulfate as substrates (at pH 8.0).

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site2794-methylumbelliferone (UniProtKB | ChEBI)
Binding site3834-methylumbelliferone (UniProtKB | ChEBI)
Active site463Nucleophile; sulfurylated histidine covalent intermediate
Binding site4634-methylumbelliferone (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentperiplasmic space
Molecular Functionaryl sulfotransferase activity
Molecular Functionarylsulfate sulfotransferase activity
Molecular Functionidentical protein binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Arylsulfate sulfotransferase AssT
  • EC number
  • Alternative names
    • Aryl sulfotransferase AssT

Gene names

    • Name
      assT
    • Ordered locus names
      c3785

Organism names

Accessions

  • Primary accession
    Q8FDI4

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis123No effect.
Mutagenesis235No effect.
Mutagenesis279Reduces enzyme activity by 96%.
Mutagenesis383Reduces enzyme activity by over 99%.
Mutagenesis401Reduces enzyme activity by over 99%.
Mutagenesis586Reduces enzyme activity by about 40%.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-27
ChainPRO_000041884428-598Arylsulfate sulfotransferase AssT
Disulfide bond445↔451

Post-translational modification

The disulfide bond is crucial for enzyme activity.

Keywords

Expression

Induction

Expressed from a tri-cistronic operon that encodes AssT, DsbI and DsbL.

Interaction

Subunit

Homodimer.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q8FDI4assT Q8FDI42EBI-15743267, EBI-15743267

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the aryl sulfotransferase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    598
  • Mass (Da)
    66,570
  • Last updated
    2003-03-01 v1
  • Checksum
    309EE9E1580D7FB0
MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIVDPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTHTFTAQGXDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014075
EMBL· GenBank· DDBJ
AAN82229.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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