Q8FA29 · Q8FA29_LEPIN
- ProteintRNA-dihydrouridine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids329 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity
- a 5,6-dihydrouridine in tRNA + NAD+ = a uridine in tRNA + H+ + NADH
- a 5,6-dihydrouridine in tRNA + NADP+ = a uridine in tRNA + H+ + NADPH
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17-19 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PMA | ||||||
Binding site | 71 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 101 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 140 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 170 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 225-226 | FMN (UniProtKB | ChEBI) | ||||
Sequence: GR |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | tRNA dihydrouridine synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine synthase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Leptospirales > Leptospiraceae > Leptospira
Accessions
- Primary accessionQ8FA29
Proteomes
PTM/Processing
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-302 | DUS-like FMN-binding | ||||
Sequence: LSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFRYKSEERPITFQIFGNRLEIIAEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILEESGVDALTVHGRTKEMAYTGFADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKKEELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGLEFDLAWKTELLEIKEWNRF |
Sequence similarities
Belongs to the dus family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length329
- Mass (Da)37,543
- Last updated2003-03-01 v1
- ChecksumC2DA18B33B5EBFF5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE010300 EMBL· GenBank· DDBJ | AAN47206.1 EMBL· GenBank· DDBJ | Genomic DNA |