Q8EHS6 · CARA_SHEON

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site51L-glutamine (UniProtKB | ChEBI)
Binding site245L-glutamine (UniProtKB | ChEBI)
Binding site247L-glutamine (UniProtKB | ChEBI)
Active site273Nucleophile
Binding site274L-glutamine (UniProtKB | ChEBI)
Binding site277L-glutamine (UniProtKB | ChEBI)
Binding site315L-glutamine (UniProtKB | ChEBI)
Binding site317L-glutamine (UniProtKB | ChEBI)
Binding site318L-glutamine (UniProtKB | ChEBI)
Active site357
Active site359

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      SO_1141

Organism names

Accessions

  • Primary accession
    Q8EHS6

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001123141-386Carbamoyl phosphate synthase small chain

Proteomic databases

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-196CPSase
Domain197-384Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    386
  • Mass (Da)
    41,347
  • Last updated
    2003-03-01 v1
  • Checksum
    1376EBE09185BC94
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDVESNGVHACGLIIRDLPLIASSFRNQQNLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEVKALAAAKAFPGLKGMDLAKEVTTDKAYPWRKGSWRLVGGLPADTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEHYRQNAK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014299
EMBL· GenBank· DDBJ
AAN54211.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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