Q8EHS6 · CARA_SHEON
- ProteinCarbamoyl phosphate synthase small chain
- GenecarA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids386 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
Catalytic activity
- hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H+
Carbamoyl phosphate synthase small chain
L-glutamine + H2O = L-glutamate + NH4+
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 51 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 245 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 247 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 273 | Nucleophile | |||
Binding site | 274 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 277 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 315 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 317 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 318 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 357 | ||||
Active site | 359 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | carbamoyl-phosphate synthase complex | |
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity | |
Molecular Function | glutaminase activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | arginine biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyl phosphate synthase small chain
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Shewanellaceae > Shewanella
Accessions
- Primary accessionQ8EHS6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000112314 | 1-386 | Carbamoyl phosphate synthase small chain | ||
Proteomic databases
Interaction
Subunit
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-196 | CPSase | |||
Domain | 197-384 | Glutamine amidotransferase type-1 | |||
Sequence similarities
Belongs to the CarA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length386
- Mass (Da)41,347
- Last updated2003-03-01 v1
- Checksum1376EBE09185BC94
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014299 EMBL· GenBank· DDBJ | AAN54211.1 EMBL· GenBank· DDBJ | Genomic DNA |