Q8DP63 · PGDA_STRR6

Function

function

Catalyzes the deacetylation of N-acetylglucosamine (GlcNAc) residues in peptidoglycan, a modification that confers host lysozyme resistance and contributes to pneumococcal virulence.

Catalytic activity

  • peptidoglycan-N-acetyl-D-glucosamine + H2O = peptidoglycan-D-glucosamine + acetate.
    EC:3.5.1.104 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Co2+ (UniProtKB | Rhea| CHEBI:48828 )

Note: Zn2+. Although displays higher activity with Co2+ than with Zn2+ in vitro, bioavailability may well limit this enzyme to be zinc-dependent.

Activity regulation

Enzymatic activity is inhibited by EDTA in vitro.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.8 mMGlcNAc3
kcat is 0.55 sec-1 with GlcNAc3 as substrate.

Features

Showing features for active site, binding site, site.

TypeIDPosition(s)Description
Active site275Proton acceptor
Binding site276Zn2+ (UniProtKB | ChEBI)
Binding site326Zn2+ (UniProtKB | ChEBI)
Binding site330Zn2+ (UniProtKB | ChEBI)
Binding site367substrate
Site391Raises pKa of active site His
Active site417Proton donor

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionlysozyme inhibitor activity
Molecular FunctionN-acetylglucosamine deacetylase activity
Molecular Functionzinc ion binding
Biological Processcarbohydrate metabolic process
Biological Processcell wall modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidoglycan-N-acetylglucosamine deacetylase
  • EC number
  • Short names
    Peptidoglycan GlcNAc deacetylase
  • Alternative names
    • Peptidoglycan N-deacetylase
      (PG N-deacetylase
      )

Gene names

    • Name
      pgdA
    • Ordered locus names
      spr1333

Organism names

Accessions

  • Primary accession
    Q8DP63

Proteomes

Subcellular Location

Cell membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane18-38Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene produce fully N-acetylated glycan, become hypersensitive to exogenous lysozyme in the stationary phase of growth, and show reduced virulence in the intraperitoneal mouse model.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis275Loss of activity.
Mutagenesis326Loss of activity.
Mutagenesis364Loss of activity.
Mutagenesis391Loss of activity.
Mutagenesis417Loss of activity.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004244401-463Peptidoglycan-N-acetylglucosamine deacetylase

Interaction

Protein-protein interaction databases

Chemistry

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain268-442NodB homology

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    463
  • Mass (Da)
    52,675
  • Last updated
    2003-03-01 v1
  • Checksum
    12E1935E66521EDE
MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQKSFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKDKLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQPFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDSQIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDDGPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILSQLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDWKSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNTRLKAHELYYSRDE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE007317
EMBL· GenBank· DDBJ
AAL00137.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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