Q8DP63 · PGDA_STRR6
- ProteinPeptidoglycan-N-acetylglucosamine deacetylase
- GenepgdA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids463 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the deacetylation of N-acetylglucosamine (GlcNAc) residues in peptidoglycan, a modification that confers host lysozyme resistance and contributes to pneumococcal virulence.
Catalytic activity
Cofactor
Co2+ (UniProtKB | Rhea| CHEBI:48828 )
Note: Zn2+. Although displays higher activity with Co2+ than with Zn2+ in vitro, bioavailability may well limit this enzyme to be zinc-dependent.
Activity regulation
Enzymatic activity is inhibited by EDTA in vitro.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.8 mM | GlcNAc3 |
kcat is 0.55 sec-1 with GlcNAc3 as substrate.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 275 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 276 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 326 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 330 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 367 | substrate | ||||
Sequence: Y | ||||||
Site | 391 | Raises pKa of active site His | ||||
Sequence: D | ||||||
Active site | 417 | Proton donor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | lysozyme inhibitor activity | |
Molecular Function | N-acetylglucosamine deacetylase activity | |
Molecular Function | zinc ion binding | |
Biological Process | carbohydrate metabolic process | |
Biological Process | cell wall modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeptidoglycan-N-acetylglucosamine deacetylase
- EC number
- Short namesPeptidoglycan GlcNAc deacetylase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionQ8DP63
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 18-38 | Helical | ||||
Sequence: LLALIGILAISICLLGGFIAF |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene produce fully N-acetylated glycan, become hypersensitive to exogenous lysozyme in the stationary phase of growth, and show reduced virulence in the intraperitoneal mouse model.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 275 | Loss of activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 326 | Loss of activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 364 | Loss of activity. | ||||
Sequence: R → S | ||||||
Mutagenesis | 391 | Loss of activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 417 | Loss of activity. | ||||
Sequence: H → S |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424440 | 1-463 | Peptidoglycan-N-acetylglucosamine deacetylase | |||
Sequence: MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQKSFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKDKLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQPFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDSQIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDDGPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILSQLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDWKSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNTRLKAHELYYSRDE |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 268-442 | NodB homology | ||||
Sequence: KVVALTFDDGPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILSQLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDWKSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFV |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length463
- Mass (Da)52,675
- Last updated2003-03-01 v1
- Checksum12E1935E66521EDE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE007317 EMBL· GenBank· DDBJ | AAL00137.1 EMBL· GenBank· DDBJ | Genomic DNA |