Q8CT28 · PUR3_STAES
- ProteinPhosphoribosylglycinamide formyltransferase
- GenepurN
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids188 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.
Catalytic activity
- (6R)-10-formyltetrahydrofolate + N1-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H+ + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-14 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: GSN | ||||||
Binding site | 66 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 91-94 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: MRLV | ||||||
Binding site | 108 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 110 | Proton donor | ||||
Sequence: H | ||||||
Site | 146 | Raises pKa of active site His | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | phosphoribosylglycinamide formyltransferase activity | |
Biological Process | 'de novo' IMP biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoribosylglycinamide formyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ8CT28
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074951 | 1-188 | Phosphoribosylglycinamide formyltransferase | |||
Sequence: MTNIAIFASGSGSNFENIVKHIQTGQLSGINVTALYTDNEGVPCIDRAKNLNIPIHINKPKDFSSKSLYEQHLLKLLSSEEVQWIVLAGYMRLVGQDLLQAYEGRILNIHPSLLPKFKGLDAIGQALESGDTVTGSTVHYVDSGMDTGEIIEQQQCDIKPDDTKEQLEDRVKHLEYELYPRVIAKIIK |
Structure
Sequence
- Sequence statusComplete
- Length188
- Mass (Da)20,965
- Last updated2003-03-01 v1
- Checksum7A0D37C071678E8A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE015929 EMBL· GenBank· DDBJ | AAO04367.1 EMBL· GenBank· DDBJ | Genomic DNA |