Q8CHG5 · AREL1_MOUSE

  • Protein
    Apoptosis-resistant E3 ubiquitin protein ligase 1
  • Gene
    Arel1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

E3 ubiquitin-protein ligase that catalyzes 'Lys-11'- or 'Lys-33'-linked polyubiquitin chains, with some preference for 'Lys-33' linkages (By similarity).
E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity).
Ubiquitinates SEPTIN4, DIABLO/SMAC and HTRA2 in vitro (By similarity).
Modulates pulmonary inflammation by targeting SOCS2 for ubiquitination and subsequent degradation by the proteasome (PubMed:31578312).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.26 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for active site.

1823100200300400500600700800
TypeIDPosition(s)Description
Active site790Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular Functionubiquitin protein ligase activity
Molecular Functionubiquitin-protein transferase activity
Biological Processapoptotic process
Biological Processnegative regulation of apoptotic process
Biological Processprotein K11-linked ubiquitination
Biological Processprotein K33-linked ubiquitination
Biological Processprotein polyubiquitination
Biological Processprotein ubiquitination
Biological Processregulation of inflammatory response
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Apoptosis-resistant E3 ubiquitin protein ligase 1
  • EC number
  • Alternative names
    • Apoptosis-resistant HECT-type E3 ubiquitin transferase 1

Gene names

    • Name
      Arel1
    • Synonyms
      Kiaa0317

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8CHG5
  • Secondary accessions
    • Q6P9Q1
    • Q80YC4
    • Q8C5W5

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Mice show significant and stepwise decreases in indicators of inflammatory injury, such as bronchoalveolar lavage fluid protein concentration and cell count, as well as decreases in pro-inflammatory cytokine release.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 36 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001203501-823Apoptosis-resistant E3 ubiquitin protein ligase 1

Post-translational modification

Autoubiquitinated in vitro in the presence of E2 enzyme UBE2D1/UBCH5A.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in brain, testis, heart, liver, lung and kidney with very low levels in skeletal muscle and spleen.

Gene expression databases

Interaction

Subunit

Interacts with SOCS2 (PubMed:31578312).
Interacts (via HECT domain) with HTRA2, DIABLO/SMAC and SEPTIN4; in the cytoplasm following induction of apoptosis (PubMed:23479728).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for repeat, region, domain.

TypeIDPosition(s)Description
Repeat64-158Filamin
Region315-345Disordered
Region483-789Interaction with SOCS2
Domain483-823HECT

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    823
  • Mass (Da)
    94,199
  • Last updated
    2005-03-29 v2
  • Checksum
    E74AAE76555A20D4
MFYVIGGIIVSVVAFFFTIKFLFELAARVVSFLQNEDRERRGDRTIYDYVRGNYLDPRSCKVSWDWKDPYEVGHSMAFRVHLFYKNGQPFPAHRPVGLRVHISHVELAVDIPVTQEVLQEPNSNVVKVAFTVRKAGRYEITVKLGGLNVAYSPYYKIFQPGMVVPSKTKIVCHFSTLVLTCGQPHTLQIVPRDEYDNPTNNSMSLRDEHSYSLAIHELGPQEEENNEVSFEKSVTSNRQTCQVFLRLTLHSRGCFHACISYQNQPINNGEFDIIVLSENEKNIVERNVSTSGVSIYFEAYLYNANNCTSTPWHLPPMHMSSSQRRPSTAIEEDDEDSPSECHTPEKVKKPKKVYCYVSPKQFSVKEFYLKIIPWRLYTFRVCPGTKFSYLGPDPVHKLLTLVVDDGIQPPVELSCKERNILAATFIRSLHKNIGGSETFQDKVNFFQRELRQVHMKRPHSKVTLKVSRHALLESSLKATRNFSISDWSKNFEVVFQDEEALDWGGPRREWFELICKALFDTTSQLFARFTDSNQALVHPNPNRPAHLRLKMYEFAGRLVGKCLYESSLGGAYKQLVRARFTRSFLAQIIGLRMHYKYFETDDPEFYKSKVCFILNNDMSEMELVFAEEKYNKSGQLDKIVELMTGGAQTPVTNANKIFYLNLLAQYRLASQVKEEVEHFLKGLNELVPENLLAIFDENELELLMCGTGDINVSDFKAHAVVVGGSWHFREKVMRWFWAVVSSLTQEELARLLQFTTGSSQLPPGGFAALCPSFQIIAAPTHSTLPTAHTCFNQLCLPTYDSYEEVHRMLQLAISEGCEGFGML

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
E9PZX5E9PZX5_MOUSEArel1502
E9PY98E9PY98_MOUSEArel1160
E9PYA6E9PYA6_MOUSEArel161
E9PYE5E9PYE5_MOUSEArel171
E9Q7I2E9Q7I2_MOUSEArel199
E9Q2W2E9Q2W2_MOUSEArel164

Sequence caution

The sequence BAC41414.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict424in Ref. 3; AAH60658

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB093230
EMBL· GenBank· DDBJ
BAC41414.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK077015
EMBL· GenBank· DDBJ
BAC36566.1
EMBL· GenBank· DDBJ
mRNA
BC049900
EMBL· GenBank· DDBJ
AAH49900.1
EMBL· GenBank· DDBJ
mRNA
BC060658
EMBL· GenBank· DDBJ
AAH60658.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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