Q8CFN2 · CDC42_RAT

  • Protein
    Cell division control protein 42 homolog
  • Gene
    Cdc42
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses (PubMed:25498153).
Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (By similarity).
Regulates cell migration (By similarity).
In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (By similarity).
Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (By similarity).
Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (PubMed:21423166, PubMed:25498153).
Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (By similarity).
Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (By similarity).

Catalytic activity

Activity regulation

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase (Probable). Inhibited by GAPs such as ARHGAP44 (PubMed:25498153).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10-17GTP (UniProtKB | ChEBI)
Binding site57-61GTP (UniProtKB | ChEBI)
Binding site115-118GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentapical part of cell
Cellular Componentcell leading edge
Cellular Componentcell periphery
Cellular Componentcell projection
Cellular Componentcell-cell junction
Cellular Componentcentrosome
Cellular Componentcytoplasm
Cellular Componentcytoplasmic ribonucleoprotein granule
Cellular Componentcytosol
Cellular Componentdendritic spine
Cellular Componentfilopodium
Cellular Componentglutamatergic synapse
Cellular ComponentGolgi membrane
Cellular ComponentGolgi transport complex
Cellular Componentlamellipodium membrane
Cellular Componentleading edge membrane
Cellular Componentmembrane
Cellular Componentmidbody
Cellular Componentmitotic spindle
Cellular Componentneuron projection
Cellular Componentneuronal cell body
Cellular Componentphagocytic vesicle
Cellular Componentplasma membrane
Cellular Componentpostsynapse
Cellular Componentprotein-containing complex
Cellular ComponentSchaffer collateral - CA1 synapse
Cellular Componentsecretory granule
Cellular Componentspindle midzone
Cellular Componentstorage vacuole
Molecular Functionapolipoprotein A-I receptor binding
Molecular FunctionG protein activity
Molecular FunctionGBD domain binding
Molecular FunctionGTP binding
Molecular FunctionGTP-dependent protein binding
Molecular FunctionGTPase activity
Molecular Functionidentical protein binding
Molecular Functionmitogen-activated protein kinase kinase kinase binding
Molecular Functionprotein kinase binding
Molecular Functionthioesterase binding
Molecular Functionubiquitin protein ligase activity
Biological Processactin cytoskeleton organization
Biological Processactin filament branching
Biological Processactin filament organization
Biological Processadherens junction organization
Biological Processcardiac conduction system development
Biological Processcardiac neural crest cell migration involved in outflow tract morphogenesis
Biological ProcessCdc42 protein signal transduction
Biological Processcell junction assembly
Biological Processcellular response to type II interferon
Biological Processdendritic cell migration
Biological Processdendritic spine morphogenesis
Biological Processembryonic heart tube development
Biological Processendocytosis
Biological Processendothelin receptor signaling pathway involved in heart process
Biological Processestablishment of cell polarity
Biological Processestablishment of epithelial cell apical/basal polarity
Biological Processestablishment of Golgi localization
Biological Processestablishment of localization in cell
Biological Processestablishment or maintenance of apical/basal cell polarity
Biological Processestablishment or maintenance of cell polarity
Biological Processfilopodium assembly
Biological ProcessGolgi organization
Biological Processheart contraction
Biological Processheart process
Biological Processintegrin-mediated signaling pathway
Biological Processmodification of synaptic structure
Biological Processmodulation by host of viral process
Biological Processnegative regulation of protein-containing complex assembly
Biological Processneuron fate determination
Biological Processneuropilin signaling pathway
Biological Processnuclear migration
Biological Processnucleus localization
Biological Processorganelle transport along microtubule
Biological Processphagocytosis, engulfment
Biological Processpositive regulation of cell growth
Biological Processpositive regulation of cell migration
Biological Processpositive regulation of cytokinesis
Biological Processpositive regulation of DNA replication
Biological Processpositive regulation of epithelial cell proliferation involved in lung morphogenesis
Biological Processpositive regulation of filopodium assembly
Biological Processpositive regulation of intracellular protein transport
Biological Processpositive regulation of JNK cascade
Biological Processpositive regulation of lamellipodium assembly
Biological Processpositive regulation of MAPK cascade
Biological Processpositive regulation of neuron apoptotic process
Biological Processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Processpositive regulation of pinocytosis
Biological Processpositive regulation of pseudopodium assembly
Biological Processpositive regulation of stress fiber assembly
Biological Processpositive regulation of substrate adhesion-dependent cell spreading
Biological Processpositive regulation of synapse structural plasticity
Biological Processprotein localization
Biological Processregulation of actin cytoskeleton organization
Biological Processregulation of attachment of spindle microtubules to kinetochore
Biological Processregulation of exocytosis
Biological Processregulation of filopodium assembly
Biological Processregulation of lamellipodium assembly
Biological Processregulation of mitotic nuclear division
Biological Processregulation of modification of postsynaptic structure
Biological Processregulation of postsynapse organization
Biological Processregulation of stress fiber assembly
Biological Processresponse to glucose
Biological Processsprouting angiogenesis
Biological Processsubmandibular salivary gland formation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cell division control protein 42 homolog
  • EC number

Gene names

    • Name
      Cdc42

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q8CFN2
  • Secondary accessions
    • Q6P9Y3
    • Q71TW5

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Midbody
Cytoplasm
Cell projection, lamellipodium membrane
; Peripheral membrane protein
Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome. In its active GTP-bound form localizes to the leading edge membrane of migrating dendritic cells.

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, modified residue, lipidation, propeptide.

TypeIDPosition(s)Description
ChainPRO_00002708291-188Cell division control protein 42 homolog
Modified residue64Phosphotyrosine; by SRC
Modified residue188Cysteine methyl ester
Lipidation188S-geranylgeranyl cysteine
PropeptidePRO_0000270830189-191Removed in mature form

Post-translational modification

Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with DOCK11/Zizimin2; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK9; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK8 (via DHR-2 domain); the interaction activates CDC42 by exchanging GDP for GTP. Interacts with IQGAP1. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1. Interacts with USP6. Interacts with NEK6. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with ITGB1BP1. Interacts with ARHGDIA; this interaction inactivates and stabilizes CDC42. Interacts with ARHGDIB; this maintains CDC42 in the inactive, GDP-bound form. Interacts in (GTP-bound form) with FNBP1L and ABI1, but only in the presence of FNBP1L. Interacts with MARCKS (By similarity).
Interacts with CD151 and ITGB1 (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO Q8CFN2BAIAP2 Q9UQB82EBI-7023929, EBI-525456

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif32-40Effector region

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q8CFN2-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    191
  • Mass (Da)
    21,259
  • Last updated
    2007-01-09 v2
  • Checksum
    51A437E22A4D8FFF
MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Q8CFN2-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 182-191: PKKSRRCVLL → TQPKRKCCIF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2JSM8A0A0G2JSM8_RATCdc42191

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict159in Ref. 1; AAF15538 and 2; AAN63806
Sequence conflict163in Ref. 1; AAF15538
Alternative sequenceVSP_022241182-191in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF205635
EMBL· GenBank· DDBJ
AAF15538.1
EMBL· GenBank· DDBJ
mRNA
AF491841
EMBL· GenBank· DDBJ
AAN63806.1
EMBL· GenBank· DDBJ
mRNA
BC060535
EMBL· GenBank· DDBJ
AAH60535.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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