Q8CAE2 · HENMT_MOUSE
- ProteinSmall RNA 2'-O-methyltransferase
- GeneHenmt1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids395 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
Catalytic activity
- S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H+ + S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 79 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 115 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 133 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 136 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 137 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 182 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Cellular Component | P granule | |
Molecular Function | metal ion binding | |
Molecular Function | O-methyltransferase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA methyltransferase activity | |
Molecular Function | small RNA 2'-O-methyltransferase activity | |
Biological Process | piRNA processing | |
Biological Process | RNA methylation | |
Biological Process | siRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSmall RNA 2'-O-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8CAE2
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 54-58 | Abolishes methyltransferase activity. | ||||
Sequence: DLGCG → NAVAV |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 29 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000304141 | 1-395 | Small RNA 2'-O-methyltransferase | |||
Sequence: MEMAESIPCNSVVGGNFKEVSPEKVIRFKPPLYKQRYQFVRDLVDRHEPKKVADLGCGDAKLLKLLKIYPCIQLLVGVDINEEKLHSNGHRLSPYLGEFVKPRDLDLTVTLYHGSVVERDSRLLGFDLITCIELIEHLDSDDLARFPDVVFGYLSPAMVVISTPNAEFNPLFPTVTLRDADHKFEWSRMEFQTWALHVANCYNYRVEFTGVGTPPAGSEHVGYCTQIGVFTKNGGKLSKPSVSQQCDQHVYKPVYTTSYPSLQQEKVLKFVLVGELLIQVDRLRLRYQRMLRDREKDRGPKPGDMDSCPAPHLLLGAVFTEAEKARIESSPKPFCEGEKFYIPLQRLLTYPKLHRLCADEDRVRSLIADSVCLSSDGSAVVVDLHNSWDYRPEEN |
Proteomic databases
PTM databases
Expression
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8CAE2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length395
- Mass (Da)44,921
- Last updated2003-03-01 v1
- ChecksumBA648D0289C2EEBE
Q8CAE2-2
- Name2
- Differences from canonical
- 9-51: Missing
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A2AED5 | A2AED5_MOUSE | Henmt1 | 262 | ||
A0A0G2JGG9 | A0A0G2JGG9_MOUSE | Henmt1 | 98 | ||
A0A0G2JFU0 | A0A0G2JFU0_MOUSE | Henmt1 | 10 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_028012 | 9-51 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 319 | in Ref. 1; BAC39480 | ||||
Sequence: F → L |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK038994 EMBL· GenBank· DDBJ | BAC30196.1 EMBL· GenBank· DDBJ | mRNA | ||
AK085587 EMBL· GenBank· DDBJ | BAC39480.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AL671894 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC099408 EMBL· GenBank· DDBJ | AAH99408.1 EMBL· GenBank· DDBJ | mRNA | ||
BC128494 EMBL· GenBank· DDBJ | AAI28495.1 EMBL· GenBank· DDBJ | mRNA | ||
BC128495 EMBL· GenBank· DDBJ | AAI28496.1 EMBL· GenBank· DDBJ | mRNA |