Q8C5Y2 · MISFA_MOUSE
- ProteinMitochondrial sheath formation-associated protein
- GeneMisfa
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Isoform Kastor
Regulates sperm development. May be involved in mitochondrial sheath formation.
Isoform Polluks
Regulates sperm development. May be involved in mitochondrial sheath formation.
Miscellaneous
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial outer membrane | |
Biological Process | cell differentiation | |
Biological Process | sperm mitochondrial sheath assembly | |
Biological Process | spermatogenesis |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMitochondrial sheath formation-associated protein
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8C5Y2
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform Kastor
Mitochondrion outer membrane ; Single-pass membrane protein
Isoform Polluks
Mitochondrion outer membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Mitochondrial intermembrane | ||||
Sequence: MIVLGW | ||||||
Topological domain | 1-7 | In isoform Q8C5Y2-2; Mitochondrial intermembrane | ||||
Sequence: MIVLGWM | ||||||
Transmembrane | 7-23 | Helical | ||||
Sequence: MLFVGLATYMGTFPEAM | ||||||
Transmembrane | 8-24 | In isoform Q8C5Y2-2; Helical | ||||
Sequence: LFVGLATYMGTFPEAMP | ||||||
Topological domain | 24-53 | Cytoplasmic | ||||
Sequence: PPTLKWKERLPGQENKARRRIQALEEELLL | ||||||
Topological domain | 25-40 | In isoform Q8C5Y2-2; Cytoplasmic | ||||
Sequence: PTLKWKERLPGQENKA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Isoform Kastor
Knockout mice are viable and manifest no substantial differences in body weight, testis weight or size, sperm count, or testicular histology. Although Kastor-deficient male mice are fertile, the number of pups produced by wild-type females after breeding with the mutant males is significantly reduced compared with that for wild-type males. The loss of Kastor induced a a nonuniform size of mitochondria and abnormal bending in spermatozoa.
Isoform Polluks
Knockout mice are viable and manifest no substantial differences in body weight, testis weight or size, sperm count, or testicular histology. Although Polluks-deficient male mice are fertile, the number of pups produced by wild-type females after breeding with the mutant males is significantly reduced compared with that for wild-type males. The loss of Polluks results in irregular elongation and flattening of mitochondria without abnormal bending in spermatozoa.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000457589 | 1-53 | Mitochondrial sheath formation-associated protein | |||
Sequence: MIVLGWMLFVGLATYMGTFPEAMPPTLKWKERLPGQENKARRRIQALEEELLL |
Proteomic databases
Expression
Tissue specificity
Isoform Kastor
Testis specific. Detected only in germ cells at the step of spermiogenesis (at protein level). Expressed during the middle steps of spermatid development.
Isoform Polluks
Testis specific. Detected only in germ cells at the step of spermiogenesis (at protein level). Expressed in the late steps of spermatid development.
Gene expression databases
Interaction
Subunit
Isoform Kastor
Interacts with VDAC3.
Isoform Polluks
Interacts with VDAC3.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative initiation.
Q8C5Y2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameKastor
- Length53
- Mass (Da)6,174
- Last updated2003-03-01 v1
- ChecksumBF59DEF89C958391
Q8C5Y2-2
- NamePolluks
- Differences from canonical
- 4-52: LGWMLFVGLATYMGTFPEAMPPTLKWKERLPGQENKARRRIQALEEELL → VLWMVLIAGTMWKGYKYPPGGTPLEVNKDDPGEPMQ
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_061801 | 4-52 | in isoform Polluks | |||
Sequence: LGWMLFVGLATYMGTFPEAMPPTLKWKERLPGQENKARRRIQALEEELL → VLWMVLIAGTMWKGYKYPPGGTPLEVNKDDPGEPMQ |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK076952 EMBL· GenBank· DDBJ | BAC36535.1 EMBL· GenBank· DDBJ | mRNA | ||
AC113001 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |