Q8C5Y2 · MISFA_MOUSE

  • Protein
    Mitochondrial sheath formation-associated protein
  • Gene
    Misfa
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Isoform Kastor

Regulates sperm development. May be involved in mitochondrial sheath formation.

Isoform Polluks

Regulates sperm development. May be involved in mitochondrial sheath formation.

Miscellaneous

Isoform Kastor and isoform Polluks are conserved only in mammals.

Caution

The locus MISFA encodes two different polypeptides Kastor and Polluks, with different transcription start sites and different ORFs.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmitochondrial outer membrane
Biological Processcell differentiation
Biological Processsperm mitochondrial sheath assembly
Biological Processspermatogenesis

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Mitochondrial sheath formation-associated protein

Gene names

    • Name
      Misfa
    • Synonyms
      Gm9999

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8C5Y2
  • Secondary accessions
    • A0A0N4SVS5

Proteomes

Organism-specific databases

Subcellular Location

Isoform Kastor

Mitochondrion outer membrane
; Single-pass membrane protein

Isoform Polluks

Mitochondrion outer membrane
; Single-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-6Mitochondrial intermembrane
Topological domain1-7In isoform Q8C5Y2-2; Mitochondrial intermembrane
Transmembrane7-23Helical
Transmembrane8-24In isoform Q8C5Y2-2; Helical
Topological domain24-53Cytoplasmic
Topological domain25-40In isoform Q8C5Y2-2; Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Isoform Kastor

Knockout mice are viable and manifest no substantial differences in body weight, testis weight or size, sperm count, or testicular histology. Although Kastor-deficient male mice are fertile, the number of pups produced by wild-type females after breeding with the mutant males is significantly reduced compared with that for wild-type males. The loss of Kastor induced a a nonuniform size of mitochondria and abnormal bending in spermatozoa.

Isoform Polluks

Knockout mice are viable and manifest no substantial differences in body weight, testis weight or size, sperm count, or testicular histology. Although Polluks-deficient male mice are fertile, the number of pups produced by wild-type females after breeding with the mutant males is significantly reduced compared with that for wild-type males. The loss of Polluks results in irregular elongation and flattening of mitochondria without abnormal bending in spermatozoa.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004575891-53Mitochondrial sheath formation-associated protein

Proteomic databases

Expression

Tissue specificity

Isoform Kastor

Testis specific. Detected only in germ cells at the step of spermiogenesis (at protein level). Expressed during the middle steps of spermatid development.

Isoform Polluks

Testis specific. Detected only in germ cells at the step of spermiogenesis (at protein level). Expressed in the late steps of spermatid development.

Gene expression databases

Interaction

Subunit

Isoform Kastor

Interacts with VDAC3.

Isoform Polluks

Interacts with VDAC3.

Protein-protein interaction databases

Miscellaneous

Family & Domains

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative initiation.

Q8C5Y2-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    Kastor
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    53
  • Mass (Da)
    6,174
  • Last updated
    2003-03-01 v1
  • Checksum
    BF59DEF89C958391
MIVLGWMLFVGLATYMGTFPEAMPPTLKWKERLPGQENKARRRIQALEEELLL

Q8C5Y2-2

  • Name
    Polluks
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 4-52: LGWMLFVGLATYMGTFPEAMPPTLKWKERLPGQENKARRRIQALEEELL → VLWMVLIAGTMWKGYKYPPGGTPLEVNKDDPGEPMQ

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0618014-52in isoform Polluks

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK076952
EMBL· GenBank· DDBJ
BAC36535.1
EMBL· GenBank· DDBJ
mRNA
AC113001
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp