Q8C5S7 · REC8_MOUSE
- ProteinMeiotic recombination protein REC8 homolog
- GeneRec8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids591 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | condensed chromosome | |
Cellular Component | condensed chromosome, centromeric region | |
Cellular Component | condensed nuclear chromosome | |
Cellular Component | kinetochore | |
Cellular Component | lateral element | |
Cellular Component | male germ cell nucleus | |
Cellular Component | meiotic cohesin complex | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | synaptonemal complex | |
Molecular Function | chromatin binding | |
Biological Process | double-strand break repair | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | fertilization | |
Biological Process | homologous chromosome pairing at meiosis | |
Biological Process | male meiosis I | |
Biological Process | meiotic cell cycle | |
Biological Process | meiotic sister chromatid cohesion | |
Biological Process | oocyte maturation | |
Biological Process | seminiferous tubule development | |
Biological Process | spermatid development | |
Biological Process | synaptonemal complex assembly |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMeiotic recombination protein REC8 homolog
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8C5S7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mice display a high mortality rate, both during embryogenesis and after birth, germ cell failure and sterility. Mutant females exhibit ovarian dysgenesis and lack ovarian follicles at reproductive maturity. Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I. Early chromosome pairing appears normal but synapsis occurs between sister chromatids rather than between homologous chromosomes.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 41 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000097879 | 1-591 | Meiotic recombination protein REC8 homolog | |||
Sequence: MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLNVNVVKTCEEILNYVLVRVQPPVAGLPRPRFSLYLSAQLQIGVIRVYFQQCQYLVEDIQHILEHLHRAQLRIRIDMEEADLPSLLLPNCLAMMETLEDAPEPFFGKMSVDPRLPSPFDIPQIRHLLEAATPEKTRKETLPEATPDPRKPDRTLATVQSPEVITLQEAEPIRMLQIEGEQDLPEISRGDLELLIAEKDDAILLEERQRGRLLRQRRASLPLDESREEPRALEGAGLVSALSPPAPAQVEGIQEALPGQVFPPEVQKMTGWEPGALLTEVTPPQELRLPAPPSTEKRLPSLQRPLPRRHRRRQLLFWDKETQISREKFEEQLQTGAHCWEYPVAQPPKRMLTSPAELFRTPTLSGWLPPELLGLWTHCAQVPQRMLRQRPQLETEETVEEERAADEEERRKTEALSEIEVLREAQEPSGPLMLSSELSLEAAEDEKSRTSLIPPEWWAWSEEGQPEPPALPMLPELPEVPMEMPPRPELSSEAVLRAVALKLQANKELDFSSLVPPLSPRKLASRVFYLLLVLSTQKILLVEQQKPYGPLLIRPGPKFP | ||||||
Modified residue | 149 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 164 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 192 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine.
Developmental stage
Expressed from 2 weeks postpartum (at protein level).
Gene expression databases
Interaction
Subunit
Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51. Forms a complex with EWSR1, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (PubMed:27932493).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 163-182 | Basic and acidic residues | ||||
Sequence: ATPEKTRKETLPEATPDPRK | ||||||
Region | 163-186 | Disordered | ||||
Sequence: ATPEKTRKETLPEATPDPRKPDRT | ||||||
Region | 316-336 | Disordered | ||||
Sequence: QELRLPAPPSTEKRLPSLQRP | ||||||
Region | 420-447 | Disordered | ||||
Sequence: QRPQLETEETVEEERAADEEERRKTEAL | ||||||
Compositional bias | 421-447 | Basic and acidic residues | ||||
Sequence: RPQLETEETVEEERAADEEERRKTEAL |
Sequence similarities
Belongs to the rad21 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length591
- Mass (Da)67,425
- Last updated2003-03-01 v1
- Checksum622986F564735700
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 49 | in Ref. 1; AAF69524 | ||||
Sequence: V → E | ||||||
Compositional bias | 163-182 | Basic and acidic residues | ||||
Sequence: ATPEKTRKETLPEATPDPRK | ||||||
Compositional bias | 421-447 | Basic and acidic residues | ||||
Sequence: RPQLETEETVEEERAADEEERRKTEAL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF262055 EMBL· GenBank· DDBJ | AAF69524.1 EMBL· GenBank· DDBJ | mRNA | ||
AK077167 EMBL· GenBank· DDBJ | BAC36657.1 EMBL· GenBank· DDBJ | mRNA | ||
AK146832 EMBL· GenBank· DDBJ | BAE27467.1 EMBL· GenBank· DDBJ | mRNA | ||
BC052155 EMBL· GenBank· DDBJ | AAH52155.1 EMBL· GenBank· DDBJ | mRNA |