Q8C5S7 · REC8_MOUSE

  • Protein
    Meiotic recombination protein REC8 homolog
  • Gene
    Rec8
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcondensed chromosome
Cellular Componentcondensed chromosome, centromeric region
Cellular Componentcondensed nuclear chromosome
Cellular Componentkinetochore
Cellular Componentlateral element
Cellular Componentmale germ cell nucleus
Cellular Componentmeiotic cohesin complex
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentsynaptonemal complex
Molecular Functionchromatin binding
Biological Processdouble-strand break repair
Biological Processdouble-strand break repair via homologous recombination
Biological Processfertilization
Biological Processhomologous chromosome pairing at meiosis
Biological Processmale meiosis I
Biological Processmeiotic cell cycle
Biological Processmeiotic sister chromatid cohesion
Biological Processoocyte maturation
Biological Processseminiferous tubule development
Biological Processspermatid development
Biological Processsynaptonemal complex assembly

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Meiotic recombination protein REC8 homolog
  • Alternative names
    • Cohesin Rec8p

Gene names

    • Name
      Rec8
    • Synonyms
      Mei8, Rec8L1

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8C5S7
  • Secondary accessions
    • Q3UIN9
    • Q9JK52

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Chromosome
Chromosome, centromere
Note: In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.

Keywords

Phenotypes & Variants

Disruption phenotype

Mice display a high mortality rate, both during embryogenesis and after birth, germ cell failure and sterility. Mutant females exhibit ovarian dysgenesis and lack ovarian follicles at reproductive maturity. Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I. Early chromosome pairing appears normal but synapsis occurs between sister chromatids rather than between homologous chromosomes.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 41 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000978791-591Meiotic recombination protein REC8 homolog
Modified residue149Phosphoserine
Modified residue164Phosphothreonine
Modified residue192Phosphoserine

Post-translational modification

Phosphorylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine.

Developmental stage

Expressed from 2 weeks postpartum (at protein level).

Gene expression databases

Interaction

Subunit

Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51. Forms a complex with EWSR1, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (PubMed:27932493).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias163-182Basic and acidic residues
Region163-186Disordered
Region316-336Disordered
Region420-447Disordered
Compositional bias421-447Basic and acidic residues

Sequence similarities

Belongs to the rad21 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    591
  • Mass (Da)
    67,425
  • Last updated
    2003-03-01 v1
  • Checksum
    622986F564735700
MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLNVNVVKTCEEILNYVLVRVQPPVAGLPRPRFSLYLSAQLQIGVIRVYFQQCQYLVEDIQHILEHLHRAQLRIRIDMEEADLPSLLLPNCLAMMETLEDAPEPFFGKMSVDPRLPSPFDIPQIRHLLEAATPEKTRKETLPEATPDPRKPDRTLATVQSPEVITLQEAEPIRMLQIEGEQDLPEISRGDLELLIAEKDDAILLEERQRGRLLRQRRASLPLDESREEPRALEGAGLVSALSPPAPAQVEGIQEALPGQVFPPEVQKMTGWEPGALLTEVTPPQELRLPAPPSTEKRLPSLQRPLPRRHRRRQLLFWDKETQISREKFEEQLQTGAHCWEYPVAQPPKRMLTSPAELFRTPTLSGWLPPELLGLWTHCAQVPQRMLRQRPQLETEETVEEERAADEEERRKTEALSEIEVLREAQEPSGPLMLSSELSLEAAEDEKSRTSLIPPEWWAWSEEGQPEPPALPMLPELPEVPMEMPPRPELSSEAVLRAVALKLQANKELDFSSLVPPLSPRKLASRVFYLLLVLSTQKILLVEQQKPYGPLLIRPGPKFP

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict49in Ref. 1; AAF69524
Compositional bias163-182Basic and acidic residues
Compositional bias421-447Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF262055
EMBL· GenBank· DDBJ
AAF69524.1
EMBL· GenBank· DDBJ
mRNA
AK077167
EMBL· GenBank· DDBJ
BAC36657.1
EMBL· GenBank· DDBJ
mRNA
AK146832
EMBL· GenBank· DDBJ
BAE27467.1
EMBL· GenBank· DDBJ
mRNA
BC052155
EMBL· GenBank· DDBJ
AAH52155.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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