Q8C5H1 · ANO4_MOUSE

  • Protein
    Anoctamin-4
  • Gene
    Ano4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (PubMed:23532839).
Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22075693).

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Catalytic activity

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionchloride channel activity
Molecular Functionphospholipid scramblase activity
Molecular Functionprotein dimerization activity
Biological Processcalcium activated galactosylceramide scrambling
Biological Processcalcium activated phosphatidylcholine scrambling
Biological Processcalcium activated phosphatidylserine scrambling
Biological Processchloride transmembrane transport
Biological Processestablishment of localization in cell

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Anoctamin-4
  • Alternative names
    • Transmembrane protein 16D

Gene names

    • Name
      Ano4
    • Synonyms
      Tmem16d

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8C5H1
  • Secondary accessions
    • Q8CA37

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Note: Shows an intracellular localization according to PubMed:22075693.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-352Cytoplasmic
Transmembrane353-373Helical
Topological domain374-424Extracellular
Transmembrane425-445Helical
Topological domain446-505Cytoplasmic
Transmembrane506-526Helical
Topological domain527-547Extracellular
Transmembrane548-568Helical
Topological domain569-595Cytoplasmic
Transmembrane596-616Helical
Topological domain617-715Extracellular
Transmembrane716-736Helical
Topological domain737-768Cytoplasmic
Transmembrane769-789Helical
Topological domain790-885Extracellular
Transmembrane886-906Helical
Topological domain907-955Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00003531901-955Anoctamin-4
Glycosylation544N-linked (GlcNAc...) asparagine
Glycosylation824N-linked (GlcNAc...) asparagine
Glycosylation837N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Predominantly expressed in neuronal tissues. Expressed at low levels in ovary, uterus, heart and brain.

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region73-97Disordered
Compositional bias80-94Polar residues

Sequence similarities

Belongs to the anoctamin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q8C5H1-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    955
  • Mass (Da)
    111,544
  • Last updated
    2008-11-04 v2
  • Checksum
    DE3F0B931B21FB6A
MEASSSGITNGKNKVFHAEGGLDLQSHQLDMQILPDGPKSDVDFSEILNAIQEMAKDVNILFDELEAVNSPCKDDDSLLHPGNLTSTSEDTSRLEAGGETVRERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLINSDIIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYCRYRDYRDPPHSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP

Q8C5H1-2

  • Name
    2
  • Note
    May be due to an intron retention.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q8C5H1-3

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q8CA69Q8CA69_MOUSEAno4190
S4R1D3S4R1D3_MOUSEAno4149
S4R1L3S4R1L3_MOUSEAno4920
S4R272S4R272_MOUSEAno4284
S4R1Z4S4R1Z4_MOUSEAno4356
S4R1Z9S4R1Z9_MOUSEAno4155

Features

Showing features for compositional bias, sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Compositional bias80-94Polar residues
Sequence conflict214in Ref. 1; BAC30429
Alternative sequenceVSP_052960245in isoform 3
Alternative sequenceVSP_052961246-955in isoform 3
Alternative sequenceVSP_052962438-439in isoform 2
Alternative sequenceVSP_052963440-955in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK039728
EMBL· GenBank· DDBJ
BAC30429.1
EMBL· GenBank· DDBJ
mRNA
AK078364
EMBL· GenBank· DDBJ
BAC37238.1
EMBL· GenBank· DDBJ
mRNA
AC124504
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC132383
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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