Q8C5H1 · ANO4_MOUSE
- ProteinAnoctamin-4
- GeneAno4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids955 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (PubMed:23532839).
Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22075693).
Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22075693).
Miscellaneous
The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)This reaction proceeds in the forward direction.
- a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(out) = a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(in)This reaction proceeds in the forward direction.
- a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)This reaction proceeds in the backward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | chloride channel activity | |
Molecular Function | phospholipid scramblase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | calcium activated galactosylceramide scrambling | |
Biological Process | calcium activated phosphatidylcholine scrambling | |
Biological Process | calcium activated phosphatidylserine scrambling | |
Biological Process | chloride transmembrane transport | |
Biological Process | establishment of localization in cell |
Keywords
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameAnoctamin-4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8C5H1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Shows an intracellular localization according to PubMed:22075693.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-352 | Cytoplasmic | ||||
Sequence: MEASSSGITNGKNKVFHAEGGLDLQSHQLDMQILPDGPKSDVDFSEILNAIQEMAKDVNILFDELEAVNSPCKDDDSLLHPGNLTSTSEDTSRLEAGGETVRERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLINSDIIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFA | ||||||
Transmembrane | 353-373 | Helical | ||||
Sequence: WLGWYTGMLFPAAFIGLFVFL | ||||||
Topological domain | 374-424 | Extracellular | ||||
Sequence: YGVTTLDHCQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDN | ||||||
Transmembrane | 425-445 | Helical | ||||
Sequence: GATVFFAVFMAVWATVFLEFW | ||||||
Topological domain | 446-505 | Cytoplasmic | ||||
Sequence: KRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVS | ||||||
Transmembrane | 506-526 | Helical | ||||
Sequence: ASGIFFMICVVIAAVFGIVIY | ||||||
Topological domain | 527-547 | Extracellular | ||||
Sequence: RVVTVSTFAAFKWALIRNNSQ | ||||||
Transmembrane | 548-568 | Helical | ||||
Sequence: VATTGTAVCINFCIIMLLNVL | ||||||
Topological domain | 569-595 | Cytoplasmic | ||||
Sequence: YEKVALLLTNLEQPRTESEWENSFTLK | ||||||
Transmembrane | 596-616 | Helical | ||||
Sequence: MFLFQFVNLNSSTFYIAFFLG | ||||||
Topological domain | 617-715 | Extracellular | ||||
Sequence: RFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYNLQPMNAYGLFDEYLE | ||||||
Transmembrane | 716-736 | Helical | ||||
Sequence: MILQFGFTTIFVAAFPLAPLL | ||||||
Topological domain | 737-768 | Cytoplasmic | ||||
Sequence: ALLNNIIEIRLDAYKFVTQWRRPLASRAKDIG | ||||||
Transmembrane | 769-789 | Helical | ||||
Sequence: IWYGILEGIGILSVITNAFVI | ||||||
Topological domain | 790-885 | Extracellular | ||||
Sequence: AITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYCRYRDYRDPPHSLAPYGYTLQFWHVLAARL | ||||||
Transmembrane | 886-906 | Helical | ||||
Sequence: AFIIVFEHLVFCIKHLISYLI | ||||||
Topological domain | 907-955 | Cytoplasmic | ||||
Sequence: PDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000353190 | 1-955 | Anoctamin-4 | |||
Sequence: MEASSSGITNGKNKVFHAEGGLDLQSHQLDMQILPDGPKSDVDFSEILNAIQEMAKDVNILFDELEAVNSPCKDDDSLLHPGNLTSTSEDTSRLEAGGETVRERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLINSDIIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYCRYRDYRDPPHSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP | ||||||
Glycosylation | 544 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 824 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 837 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Predominantly expressed in neuronal tissues. Expressed at low levels in ovary, uterus, heart and brain.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 73-97 | Disordered | ||||
Sequence: KDDDSLLHPGNLTSTSEDTSRLEAG | ||||||
Compositional bias | 80-94 | Polar residues | ||||
Sequence: HPGNLTSTSEDTSRL |
Sequence similarities
Belongs to the anoctamin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q8C5H1-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length955
- Mass (Da)111,544
- Last updated2008-11-04 v2
- ChecksumDE3F0B931B21FB6A
Q8C5H1-2
- Name2
- NoteMay be due to an intron retention.
Q8C5H1-3
- Name3
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Features
Showing features for compositional bias, sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 80-94 | Polar residues | ||||
Sequence: HPGNLTSTSEDTSRL | ||||||
Sequence conflict | 214 | in Ref. 1; BAC30429 | ||||
Sequence: P → H | ||||||
Alternative sequence | VSP_052960 | 245 | in isoform 3 | |||
Sequence: H → Q | ||||||
Alternative sequence | VSP_052961 | 246-955 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052962 | 438-439 | in isoform 2 | |||
Sequence: AT → GK | ||||||
Alternative sequence | VSP_052963 | 440-955 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK039728 EMBL· GenBank· DDBJ | BAC30429.1 EMBL· GenBank· DDBJ | mRNA | ||
AK078364 EMBL· GenBank· DDBJ | BAC37238.1 EMBL· GenBank· DDBJ | mRNA | ||
AC124504 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC132383 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |