Q8BVH9 · METL6_MOUSE

  • Protein
    tRNA N(3)-methylcytidine methyltransferase METTL6
  • Gene
    Mettl6
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

S-adenosyl-L-methionine-dependent methyltransferase that mediates N3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Ser), including tRNA(Ser)(UGA) and tRNA(Ser)(GCU) (PubMed:28655767).
Interaction with SARS1/SerRS is required for N3-methylcytidine methylation (By similarity).

Catalytic activity

Features

Showing features for binding site.

128220406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site45S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site49S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site87S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site110S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site136S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site137S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site157S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Functionenzyme binding
Molecular FunctiontRNA (cytidine-3-)-methyltransferase activity
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA N(3)-methylcytidine methyltransferase METTL6
  • EC number
  • Alternative names
    • Methyltransferase-like protein 6

Gene names

    • Name
      Mettl6

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8BVH9
  • Secondary accessions
    • Q9D9M6
    • Q9DAX6

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Mice were born with normal Mendelian ratio without developmental defects (PubMed:28655767, PubMed:32923617).
However, male show a significant reduction in body weight over time due to reduced energy expenditure (PubMed:32923617).
Cells show reduced N3-methylcytidine modification in tRNA fractions (PubMed:28655767).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002044551-282tRNA N3-methylcytidine methyltransferase METTL6

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer (By similarity).
Interacts with SARS1/SerRS; interaction is mediated via tRNA(Ser) and is required for N3-methylcytidine methylation (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q8BVH9-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    282
  • Mass (Da)
    32,786
  • Last updated
    2003-10-03 v2
  • Checksum
    D537C99EB4D539C6
MASFQRKGLQARILSTEEEEKLKRDQALVSAFKQQKLEKEAQKNWDLFYKRNSTNFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPLLEEDLNLFAYACDFSPRAVDYVKQHPLYNAERCKVFQCDLTRDDLLDHVPPESVDAVTLIFVLSAVHPEKMRLVLLNVYKVLKPGRSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFVDAGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKPPKDPAPTSDSASL

Q8BVH9-2

  • Name
    2
  • Note
    Due to intron retention.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 225-282: EFLAQLFVDAGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKPPKDPAPTSDSASL → GKRNGVSP

Q8BVH9-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2I3BQ54A0A2I3BQ54_MOUSEMettl6186
A0A2I3BQH6A0A2I3BQH6_MOUSEMettl678
A0A2I3BR75A0A2I3BR75_MOUSEMettl653
A0A2I3BRX7A0A2I3BRX7_MOUSEMettl6255

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_00848276-120in isoform 3
Alternative sequenceVSP_008481225-282in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK005448
EMBL· GenBank· DDBJ
BAB24041.1
EMBL· GenBank· DDBJ
mRNA
AK006715
EMBL· GenBank· DDBJ
BAB24713.1
EMBL· GenBank· DDBJ
mRNA
AK078185
EMBL· GenBank· DDBJ
BAC37165.1
EMBL· GenBank· DDBJ
mRNA
BC030449
EMBL· GenBank· DDBJ
AAH30449.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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