Q8BVH9 · METL6_MOUSE
- ProteintRNA N(3)-methylcytidine methyltransferase METTL6
- GeneMettl6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids282 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
S-adenosyl-L-methionine-dependent methyltransferase that mediates N3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Ser), including tRNA(Ser)(UGA) and tRNA(Ser)(GCU) (PubMed:28655767).
Interaction with SARS1/SerRS is required for N3-methylcytidine methylation (By similarity).
Interaction with SARS1/SerRS is required for N3-methylcytidine methylation (By similarity).
Catalytic activity
- cytidine32 in tRNA(Ser) + S-adenosyl-L-methionine = H+ + N3-methylcytidine32 in tRNA(Ser) + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 45 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 49 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 87 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 110 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 136 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 137 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 157 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | enzyme binding | |
Molecular Function | tRNA (cytidine-3-)-methyltransferase activity | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N(3)-methylcytidine methyltransferase METTL6
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8BVH9
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Mice were born with normal Mendelian ratio without developmental defects (PubMed:28655767, PubMed:32923617).
However, male show a significant reduction in body weight over time due to reduced energy expenditure (PubMed:32923617).
Cells show reduced N3-methylcytidine modification in tRNA fractions (PubMed:28655767).
However, male show a significant reduction in body weight over time due to reduced energy expenditure (PubMed:32923617).
Cells show reduced N3-methylcytidine modification in tRNA fractions (PubMed:28655767).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000204455 | 1-282 | tRNA N3-methylcytidine methyltransferase METTL6 | |||
Sequence: MASFQRKGLQARILSTEEEEKLKRDQALVSAFKQQKLEKEAQKNWDLFYKRNSTNFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPLLEEDLNLFAYACDFSPRAVDYVKQHPLYNAERCKVFQCDLTRDDLLDHVPPESVDAVTLIFVLSAVHPEKMRLVLLNVYKVLKPGRSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFVDAGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKPPKDPAPTSDSASL |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q8BVH9-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length282
- Mass (Da)32,786
- Last updated2003-10-03 v2
- ChecksumD537C99EB4D539C6
Q8BVH9-2
- Name2
- NoteDue to intron retention.
- Differences from canonical
- 225-282: EFLAQLFVDAGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKPPKDPAPTSDSASL → GKRNGVSP
Q8BVH9-3
- Name3
- Differences from canonical
- 76-120: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2I3BQ54 | A0A2I3BQ54_MOUSE | Mettl6 | 186 | ||
A0A2I3BQH6 | A0A2I3BQH6_MOUSE | Mettl6 | 78 | ||
A0A2I3BR75 | A0A2I3BR75_MOUSE | Mettl6 | 53 | ||
A0A2I3BRX7 | A0A2I3BRX7_MOUSE | Mettl6 | 255 |
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK005448 EMBL· GenBank· DDBJ | BAB24041.1 EMBL· GenBank· DDBJ | mRNA | ||
AK006715 EMBL· GenBank· DDBJ | BAB24713.1 EMBL· GenBank· DDBJ | mRNA | ||
AK078185 EMBL· GenBank· DDBJ | BAC37165.1 EMBL· GenBank· DDBJ | mRNA | ||
BC030449 EMBL· GenBank· DDBJ | AAH30449.1 EMBL· GenBank· DDBJ | mRNA |