Q8BTW8 · CK5P1_MOUSE
- ProteinMitochondrial tRNA methylthiotransferase CDK5RAP1
- GeneCdk5rap1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids588 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i6A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:25738458).
Essential for efficient and highly accurate protein translation by the ribosome (PubMed:25738458).
Specifically inhibits CDK5 activation by CDK5R1 (By similarity).
Essential for efficient mitochondrial protein synthesis and respiratory chain (PubMed:25738458).
Essential for efficient and highly accurate protein translation by the ribosome (PubMed:25738458).
Specifically inhibits CDK5 activation by CDK5R1 (By similarity).
Essential for efficient mitochondrial protein synthesis and respiratory chain (PubMed:25738458).
Catalytic activity
- [sulfur carrier]-SH + AH2 + N6-dimethylallyladenosine37 in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 108 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 144 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 182 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 257 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 261 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 264 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | nucleoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | N6-isopentenyladenosine methylthiotransferase activity | |
Molecular Function | protein-containing complex binding | |
Biological Process | mitochondrial tRNA modification | |
Biological Process | negative regulation of cyclin-dependent protein serine/threonine kinase activity | |
Biological Process | positive regulation of mitochondrial translation | |
Biological Process | positive regulation of translational fidelity | |
Biological Process | regulation of cyclin-dependent protein serine/threonine kinase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMitochondrial tRNA methylthiotransferase CDK5RAP1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8BTW8
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Knockout mice show a deficiency in ms2i6A modification, resulting in impaired mitochondrial protein synthesis, which leads to respiratory defects.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-30 | Mitochondrion | ||||
Sequence: MHPLRCVLQVQRLSAPFTSMCWVLLRTCRA | ||||||
Chain | PRO_0000141765 | 31-588 | Mitochondrial tRNA methylthiotransferase CDK5RAP1 | |||
Sequence: QSSVSSTPCPSPEAKSSEAQKDFSSRLATGPTFQHFLRSASVPQEKPSSPEVEDPPPYLSGDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILCRTTMKDSLTYCTT |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in brain, liver, skeletal muscle and heart.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 33-53 | Disordered | ||||
Sequence: SVSSTPCPSPEAKSSEAQKDF | ||||||
Region | 70-91 | Disordered | ||||
Sequence: ASVPQEKPSSPEVEDPPPYLSG | ||||||
Domain | 99-219 | MTTase N-terminal | ||||
Sequence: RKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDLPRLLAVVES | ||||||
Domain | 243-498 | Radical SAM core | ||||
Sequence: SPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKA | ||||||
Domain | 500-575 | TRAM | ||||
Sequence: KTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILC |
Sequence similarities
Belongs to the methylthiotransferase family. MiaB subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length588
- Mass (Da)66,110
- Last updated2003-06-16 v2
- Checksum4430C4F1A067093C
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q3UW67 | Q3UW67_MOUSE | Cdk5rap1 | 190 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 505 | in Ref. 1; BAC40398 | ||||
Sequence: C → Y |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK010097 EMBL· GenBank· DDBJ | BAB26700.1 EMBL· GenBank· DDBJ | mRNA | ||
AK088517 EMBL· GenBank· DDBJ | BAC40398.1 EMBL· GenBank· DDBJ | mRNA | ||
BC025132 EMBL· GenBank· DDBJ | AAH25132.1 EMBL· GenBank· DDBJ | mRNA |