Q8BME9 · CBLN4_MOUSE
- ProteinCerebellin-4
- GeneCbln4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids198 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a synaptic organizer in specific subsets of neurons in the brain (PubMed:29691328).
Essential for the formation and maintenance of inhibitory GABAergic synapses (PubMed:25534236).
Promotes the development of dendrite-targeting inhibitory GABAergic synapses made by somatostatin-positive interneurons (PubMed:30679375).
May contribute to the function of ventral medial habenula region of the brain implicated in the regulation of anxiety-related behaviors (PubMed:30287486).
May play a role in CBLN3 export from the endoplasmic reticulum and secretion (PubMed:17030622).
Essential for the formation and maintenance of inhibitory GABAergic synapses (PubMed:25534236).
Promotes the development of dendrite-targeting inhibitory GABAergic synapses made by somatostatin-positive interneurons (PubMed:30679375).
May contribute to the function of ventral medial habenula region of the brain implicated in the regulation of anxiety-related behaviors (PubMed:30287486).
May play a role in CBLN3 export from the endoplasmic reticulum and secretion (PubMed:17030622).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | extracellular space | |
Cellular Component | GABA-ergic synapse | |
Cellular Component | glutamatergic synapse | |
Cellular Component | synapse | |
Cellular Component | synaptic cleft | |
Biological Process | inhibitory synapse assembly | |
Biological Process | protein secretion | |
Biological Process | trans-synaptic signaling, modulating synaptic transmission |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCerebellin-4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8BME9
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Mutant animals are fertile, have normal life spans and have no overt anatomical abnormalities (PubMed:22220752).
They have a normal corpus callosum, hippocampal commisure and pontine nucleus and no other gross neuroanatomical abnormalities (PubMed:22220752).
Mice exhibit increased anxiety-related behavior (PubMed:30287486).
Triple CBLN1, CBLN2 and CBLN4 knockout mice exhibit impairments in sensory processing and sensorimotor gating, in addition to severe motor deficits, seizures and reduced synapse density in the hippocampus of aging mice (PubMed:29691328).
They have a normal corpus callosum, hippocampal commisure and pontine nucleus and no other gross neuroanatomical abnormalities (PubMed:22220752).
Mice exhibit increased anxiety-related behavior (PubMed:30287486).
Triple CBLN1, CBLN2 and CBLN4 knockout mice exhibit impairments in sensory processing and sensorimotor gating, in addition to severe motor deficits, seizures and reduced synapse density in the hippocampus of aging mice (PubMed:29691328).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 26 | No effect on its ability to form homohexameric or heteromeric complexes with other CBLN family members. Increased interaction with NRXN1, NRXN3 and DCC. Shows interaction with NEO1, GRID1 and GRID2. Total loss of N-glycosylation; when associated with Q-85. | ||||
Sequence: N → Q | ||||||
Mutagenesis | 85 | No effect on its ability to form homohexameric or heteromeric complexes with other CBLN family members. Increased interaction with NRXN1, NRXN3 and DCC. Shows interaction with NEO1, GRID1 and GRID2. Total loss of N-glycosylation; when associated with Q-26. | ||||
Sequence: N → Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MGSARRALSVVPAVLLILVLPVWA | ||||||
Chain | PRO_0000003557 | 25-198 | Cerebellin-4 | |||
Sequence: QNDTEPIVLEGKCLVVCDSNPATDSKGSSSSPLGISVRAANSKVAFSAVRSTNHEPSEMSNKTRIIYFDQILVNVGNFFTLESVFVAPRKGIYSFSFHVIKVYQSQTIQVNLMLNGKPVISAFAGDKDVTREAATNGVLLYLDKEDKVYLKLEKGNLLGGWQYSTFSGFLVFPL | ||||||
Glycosylation | 26 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 37 | Interchain | ||||
Sequence: C | ||||||
Disulfide bond | 41 | Interchain | ||||
Sequence: C | ||||||
Glycosylation | 85 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
Sialoglycoprotein.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in brain with high levels in particular thalamic nuclei. In the thalamus, predominantly expressed in neurons within the parafascicular nucleus. Found in the hippocampus, mostly in the dendrites and somata of pyramidal neurons (at protein level). Very low or no expression in most other brain regions. Highly expressed in the ventral medial habenula.
Developmental stage
In the developing brain, expressed as early as 10-13 dpc. Expression level peaks at 18 dpc and gradually decreases afterwards. Expressed in developing somatostatin-positive basket cells.
Gene expression databases
Interaction
Subunit
Homohexamer; disulfide-linked homotrimers. The trimers are assembled via the globular C1q domains. The trimers associate via N-terminal cysteine residues to form disulfide-linked hexamers (By similarity).
May form oligomers with CBLN1, CBLN2 and CBLN3 prior to secretion (PubMed:17030622, PubMed:29782851).
Once secreted, does not interact with other CBLN family members (PubMed:17030622).
Strongly interacts with DCC in a NTN1-displaceable fashion (PubMed:22220752, PubMed:29782851).
Weakly binds to NRXN1 and NRXN2 long and short isoforms produced by alternative promoter usage (PubMed:22220752, PubMed:29782851).
Interaction with NRXN3 short isoform is hardly detectable; no interaction at all with NRXN3 long isoform (PubMed:22220752, PubMed:29782851).
Does not interact with NEO1, GRID1 and GRID2 (PubMed:22220752, PubMed:29782851).
May form oligomers with CBLN1, CBLN2 and CBLN3 prior to secretion (PubMed:17030622, PubMed:29782851).
Once secreted, does not interact with other CBLN family members (PubMed:17030622).
Strongly interacts with DCC in a NTN1-displaceable fashion (PubMed:22220752, PubMed:29782851).
Weakly binds to NRXN1 and NRXN2 long and short isoforms produced by alternative promoter usage (PubMed:22220752, PubMed:29782851).
Interaction with NRXN3 short isoform is hardly detectable; no interaction at all with NRXN3 long isoform (PubMed:22220752, PubMed:29782851).
Does not interact with NEO1, GRID1 and GRID2 (PubMed:22220752, PubMed:29782851).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 63-198 | C1q | ||||
Sequence: AANSKVAFSAVRSTNHEPSEMSNKTRIIYFDQILVNVGNFFTLESVFVAPRKGIYSFSFHVIKVYQSQTIQVNLMLNGKPVISAFAGDKDVTREAATNGVLLYLDKEDKVYLKLEKGNLLGGWQYSTFSGFLVFPL |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length198
- Mass (Da)21,609
- Last updated2003-03-01 v1
- Checksum5A35ACC1354C70D6
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 112 | in Ref. 1; BAC27855 | ||||
Sequence: P → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY134663 EMBL· GenBank· DDBJ | AAN08614.1 EMBL· GenBank· DDBJ | mRNA | ||
AK032406 EMBL· GenBank· DDBJ | BAC27855.1 EMBL· GenBank· DDBJ | mRNA | ||
AK032621 EMBL· GenBank· DDBJ | BAC27954.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466551 EMBL· GenBank· DDBJ | EDL06594.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC094540 EMBL· GenBank· DDBJ | AAH94540.1 EMBL· GenBank· DDBJ | mRNA | ||
BC132025 EMBL· GenBank· DDBJ | AAI32026.1 EMBL· GenBank· DDBJ | mRNA | ||
BC132027 EMBL· GenBank· DDBJ | AAI32028.1 EMBL· GenBank· DDBJ | mRNA |