Q8BIW9 · CTF18_MOUSE
- ProteinChromosome transmission fidelity protein 18 homolog
- GeneChtf18
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids969 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Ctf18 RFC-like complex | |
Cellular Component | cytoplasm | |
Cellular Component | male germ cell nucleus | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Biological Process | chromosome segregation | |
Biological Process | DNA duplex unwinding | |
Biological Process | DNA replication | |
Biological Process | germ cell development | |
Biological Process | male meiotic nuclear division | |
Biological Process | negative regulation of chromosome segregation | |
Biological Process | positive regulation of DNA-directed DNA polymerase activity | |
Biological Process | reciprocal meiotic recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameChromosome transmission fidelity protein 18 homolog
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8BIW9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associates with chromatin during S phase.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000340082 | 1-969 | Chromosome transmission fidelity protein 18 homolog | |||
Sequence: MEDYEEDLYGVEDDFQNQFAAELEVLAELEGTRDQAPPGTLQTPASRPPLTFEEAIAGGDTVPRPCPAGSPGNVNRNTRKNVRRDQPAPSSPMVKRPRLDVVKKLNFEPDMEELLYPDSPPGDITPPPSPEVFPEMLDAGYSDANADKDLMQTLPSPRNRNPVLRRPPILEDYINVTSTSGERAFLVLRADLIGPGVQNPLLDVHWRGCGQLDLLGVPFASLKEQVDSKRRQQLLEDAQQLSDTLHSLRSEGEEAVLEGPPAEEPAPGQNTAQHCLWVDEFAPQHYTELLSDDFTNRCLLKWLKLWDLVVFGRERPARKPRPGVETTRVGKEATAPGKWKSHEQALEEMLEAELDPSQRPRQKVALLCGPPGLGKTTLAHVVARHAGYCVVEMNASDDRSPEAFRTRIEAATQMESVLGVGGRPNCLVIDEIDGAPTAAINVLLGILNRKGPQEADQGGTAVAAGGRRRRAEGGLLTRPIICICNDQFTPSLRQLKQQALLLHVPPTLPSRLVQRLQEISLQHGMRSDPGALVALCEKTDNDIRACINTLQFLYGRGRRELSVKAVQTTHVGLKDQRKGLFSVWQEVFQLPRTQRRIVGQDLMLPAHALLLSNGDKGSLTLASQRFYHILRVTTSAGEHEKVVQGLFDNFLRLRLRDSSLSTVCCALDWLAFDDLLEQAAHRGQSFQLLCYLPFVPAAFHVLFASSHVPRITFPSSQQEAQTRMSQTRNHIQTLVSGMAPTTRSRATPQALVLDTLCLLLDVLAPKLRPVSTQLYSAHEKQQLSCLVGTMLAYSLTYHQERTPDGQYLYKLEPNVEEVCRFPELPARKPLTYQAKQLIAREIEMEKMRRAEALAWARSGPQVDQGSSGPASLWTDSGEKGTRQPAPRNHEQRLEHIMKRATVQEQPERDFFGRVVIRKVAVPSREVEAPQKDADEWRMGVAVGRSEVWFRFNEGVSNAVRRSLYIRDLL | ||||||
Modified residue | 51 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 221 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. During assembly of the CTF18-RFC complex, CTF18 may first assemble into a subcomplex with RFC2, RFC3, RFC4 and RFC5. CTF18 then interacts directly with CTF8, which in turn interacts with DCC1. The CTF18-RFC complex associates with PCNA and with DNA polymerase POLH. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. CTF18 interacts with SMC1A and RAD21. Interacts with DDX11.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 30-97 | Disordered | ||||
Sequence: EGTRDQAPPGTLQTPASRPPLTFEEAIAGGDTVPRPCPAGSPGNVNRNTRKNVRRDQPAPSSPMVKRP | ||||||
Region | 250-269 | Disordered | ||||
Sequence: SEGEEAVLEGPPAEEPAPGQ | ||||||
Region | 318-340 | Disordered | ||||
Sequence: RKPRPGVETTRVGKEATAPGKWK | ||||||
Region | 856-889 | Disordered | ||||
Sequence: ARSGPQVDQGSSGPASLWTDSGEKGTRQPAPRNH | ||||||
Compositional bias | 860-878 | Polar residues | ||||
Sequence: PQVDQGSSGPASLWTDSGE |
Sequence similarities
Belongs to the activator 1 small subunits family. CTF18 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length969
- Mass (Da)108,137
- Last updated2008-06-10 v2
- ChecksumE0269C507AF514BD
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E9PVJ3 | E9PVJ3_MOUSE | Chtf18 | 970 | ||
A0A0R4J0I4 | A0A0R4J0I4_MOUSE | Chtf18 | 969 | ||
E9Q2M3 | E9Q2M3_MOUSE | Chtf18 | 365 | ||
F6ZBE7 | F6ZBE7_MOUSE | Chtf18 | 130 | ||
F6YVH6 | F6YVH6_MOUSE | Chtf18 | 109 |
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 860-878 | Polar residues | ||||
Sequence: PQVDQGSSGPASLWTDSGE | ||||||
Sequence conflict | 880 | in Ref. 2; AAH24142 | ||||
Sequence: G → E | ||||||
Sequence conflict | 885 | in Ref. 1; BAC37079 | ||||
Sequence: A → V | ||||||
Sequence conflict | 898 | in Ref. 1; BAC37079 | ||||
Sequence: K → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK077947 EMBL· GenBank· DDBJ | BAC37079.1 EMBL· GenBank· DDBJ | mRNA | ||
BC024142 EMBL· GenBank· DDBJ | AAH24142.1 EMBL· GenBank· DDBJ | mRNA |