Q8BFR6 · ZFAN1_MOUSE

  • Protein
    AN1-type zinc finger protein 1
  • Gene
    Zfand1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Plays a role in the regulation of cytoplasmic stress granules (SGs) turnover. SGs are dynamic and transient cytoplasmic ribonucleoprotein assemblies important for cellular protein homeostasis when protein production is suspended after acute exogenous stress. Associates with SGs and is involved in the efficient and specific arsenite-induced clearance process of SGs through the recruitment of the ubiquitin-selective ATPase VCP and the 26S proteasome. This process requires both complexes for efficient degradation of damaged ubiquitinated SG proteins during recovery from arsenite stress, and hence avoiding aberrant cytoplasmic SGs degradation via autophagy.

Features

Showing features for binding site.

126820406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site10Zn2+ 1 (UniProtKB | ChEBI)
Binding site15Zn2+ 1 (UniProtKB | ChEBI)
Binding site25Zn2+ 2 (UniProtKB | ChEBI)
Binding site28Zn2+ 2 (UniProtKB | ChEBI)
Binding site33Zn2+ 1 (UniProtKB | ChEBI)
Binding site36Zn2+ 1 (UniProtKB | ChEBI)
Binding site42Zn2+ 2 (UniProtKB | ChEBI)
Binding site44Zn2+ 2 (UniProtKB | ChEBI)
Binding site64Zn2+ 3 (UniProtKB | ChEBI)
Binding site69Zn2+ 3 (UniProtKB | ChEBI)
Binding site79Zn2+ 4 (UniProtKB | ChEBI)
Binding site82Zn2+ 4 (UniProtKB | ChEBI)
Binding site87Zn2+ 3 (UniProtKB | ChEBI)
Binding site90Zn2+ 3 (UniProtKB | ChEBI)
Binding site96Zn2+ 4 (UniProtKB | ChEBI)
Binding site98Zn2+ 4 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasmic stress granule
Molecular Functionproteasome binding
Molecular Functionzinc ion binding
Biological Processcellular response to arsenite ion
Biological Processpositive regulation of intracellular protein transport
Biological Processstress granule disassembly

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    AN1-type zinc finger protein 1
  • Alternative names
    • Zinc finger AN1-type-containing protein 1

Gene names

    • Name
      Zfand1

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8BFR6
  • Secondary accessions
    • Q8R3Y6
    • Q9D7H5

Proteomes

Organism-specific databases

Subcellular Location

Note: Colocalizes with TIA1, G3BP1, VCP and 26S proteasome in cytoplasmic stress granules (SGs) in response to arsenite-induced stress treatment in a VCP-independent manner. Not localized in SGs in response to other heat- oxidative- or osmotic-induced stress treatments. Colocalizes with VCP in cytoplasmic speckles.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000665811-268AN1-type zinc finger protein 1

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Associates with the 26S proteasome; this association occurs upon exposure to arsenite and is reduced in the presence of ATP. Interacts (via AN1-type 1 and 2 zinc fingers) with PSMD1; this interaction is increased upon arsenite treatment and occurs in an ATP-independent manner. Interacts with PSMC4. Interacts with PSMA1. Interacts (via its ubiquitin-like region) with VCP; this interaction occurs in an arsenite-dependent manner and is necessary for the recruitment of the ubiquitin-selective ATPase VCP to stress granules (SGs).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for zinc finger, region.

Type
IDPosition(s)Description
Zinc finger4-52AN1-type 1
Zinc finger58-106AN1-type 2
Region160-260ubiquitin-like

Domain

The ubiquitin-like region is necessary for its localization to stress granules (SGs) in a VCP-independent manner. The AN1-type 1 and 2 zinc finger domains are necessary for the recruitment of the 26S proteasome to SGs. Both the AN1-type 1 and 2 zinc finger domains and the ubiquitin-like region are necessary for efficient SGs clearance upon specific arsenite-induced responses.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    268
  • Mass (Da)
    30,203
  • Last updated
    2003-03-01 v1
  • MD5 Checksum
    2CA9C8E8C45141999C4355ABE0800CA0
MAELDIGQHCQVQHCRQRDFLPFVCDGCSGIFCLEHRSKDSHGCSEVNVVKERPKTDEHKSYSCSFKGCTDVELVAVICPYCEKNFCLRHRHQSDHDCEKLEVAKPRMAATQKLVRDIVDAKTGGAASKGRKGAKSSGTAAKVALMKLKMHADGDKSLPQTERTYFQVYLPKGSKEKSKAMFFCLRWSIGKVVDFAASLANLRNENNKLTAKKLRLCHVPSGEALPLDHTLERWITKEECPLYNGGNVILEYLNDEEQFLKNVDSYLE

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
V9GXT9V9GXT9_MOUSEZfand149
D3Z7G5D3Z7G5_MOUSEZfand1207
F7CFT1F7CFT1_MOUSEZfand1247

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict102in Ref. 1; BAB26161

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK009240
EMBL· GenBank· DDBJ
BAB26161.1
EMBL· GenBank· DDBJ
mRNA
AK030472
EMBL· GenBank· DDBJ
BAC26977.1
EMBL· GenBank· DDBJ
mRNA
AK077873
EMBL· GenBank· DDBJ
BAC37044.1
EMBL· GenBank· DDBJ
mRNA
BC023352
EMBL· GenBank· DDBJ
AAH23352.1
EMBL· GenBank· DDBJ
mRNA
BC046585
EMBL· GenBank· DDBJ
AAH46585.1
EMBL· GenBank· DDBJ
mRNA
BC089494
EMBL· GenBank· DDBJ
AAH89494.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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