Q8BFR6 · ZFAN1_MOUSE
- ProteinAN1-type zinc finger protein 1
- GeneZfand1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids268 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Plays a role in the regulation of cytoplasmic stress granules (SGs) turnover. SGs are dynamic and transient cytoplasmic ribonucleoprotein assemblies important for cellular protein homeostasis when protein production is suspended after acute exogenous stress. Associates with SGs and is involved in the efficient and specific arsenite-induced clearance process of SGs through the recruitment of the ubiquitin-selective ATPase VCP and the 26S proteasome. This process requires both complexes for efficient degradation of damaged ubiquitinated SG proteins during recovery from arsenite stress, and hence avoiding aberrant cytoplasmic SGs degradation via autophagy.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 10 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 15 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 25 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 28 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 33 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 36 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 42 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 44 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 64 | Zn2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 69 | Zn2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 79 | Zn2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 82 | Zn2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 87 | Zn2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 90 | Zn2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 96 | Zn2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 98 | Zn2+ 4 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic stress granule | |
Molecular Function | proteasome binding | |
Molecular Function | zinc ion binding | |
Biological Process | cellular response to arsenite ion | |
Biological Process | positive regulation of intracellular protein transport | |
Biological Process | stress granule disassembly |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameAN1-type zinc finger protein 1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8BFR6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with TIA1, G3BP1, VCP and 26S proteasome in cytoplasmic stress granules (SGs) in response to arsenite-induced stress treatment in a VCP-independent manner. Not localized in SGs in response to other heat- oxidative- or osmotic-induced stress treatments. Colocalizes with VCP in cytoplasmic speckles.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000066581 | 1-268 | AN1-type zinc finger protein 1 | ||
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Associates with the 26S proteasome; this association occurs upon exposure to arsenite and is reduced in the presence of ATP. Interacts (via AN1-type 1 and 2 zinc fingers) with PSMD1; this interaction is increased upon arsenite treatment and occurs in an ATP-independent manner. Interacts with PSMC4. Interacts with PSMA1. Interacts (via its ubiquitin-like region) with VCP; this interaction occurs in an arsenite-dependent manner and is necessary for the recruitment of the ubiquitin-selective ATPase VCP to stress granules (SGs).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Zinc finger | 4-52 | AN1-type 1 | |||
Zinc finger | 58-106 | AN1-type 2 | |||
Region | 160-260 | ubiquitin-like | |||
Domain
The ubiquitin-like region is necessary for its localization to stress granules (SGs) in a VCP-independent manner. The AN1-type 1 and 2 zinc finger domains are necessary for the recruitment of the 26S proteasome to SGs. Both the AN1-type 1 and 2 zinc finger domains and the ubiquitin-like region are necessary for efficient SGs clearance upon specific arsenite-induced responses.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length268
- Mass (Da)30,203
- Last updated2003-03-01 v1
- MD5 Checksum2CA9C8E8C45141999C4355ABE0800CA0
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 102 | in Ref. 1; BAB26161 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK009240 EMBL· GenBank· DDBJ | BAB26161.1 EMBL· GenBank· DDBJ | mRNA | ||
AK030472 EMBL· GenBank· DDBJ | BAC26977.1 EMBL· GenBank· DDBJ | mRNA | ||
AK077873 EMBL· GenBank· DDBJ | BAC37044.1 EMBL· GenBank· DDBJ | mRNA | ||
BC023352 EMBL· GenBank· DDBJ | AAH23352.1 EMBL· GenBank· DDBJ | mRNA | ||
BC046585 EMBL· GenBank· DDBJ | AAH46585.1 EMBL· GenBank· DDBJ | mRNA | ||
BC089494 EMBL· GenBank· DDBJ | AAH89494.1 EMBL· GenBank· DDBJ | mRNA |