Q8BDE6 · NCAP_LATVB
- ProteinNucleoprotein
- GeneN
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids557 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 379 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 381 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 389 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Site | 456 | Important for exonuclease activity | ||||
Sequence: D | ||||||
Binding site | 496 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 499 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 518 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 522 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | helical viral capsid | |
Cellular Component | host cell cytoplasm | |
Cellular Component | ribonucleoprotein complex | |
Cellular Component | viral nucleocapsid | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Biological Process | negative stranded viral RNA replication | |
Biological Process | RNA-templated viral transcription | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleoprotein
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Arenaviridae > Mammarenavirus
- Virus hosts
Accessions
- Primary accessionQ8BDE6
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 214 | |||||
Sequence: D → N | ||||||
Natural variant | 279 | |||||
Sequence: V → I |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000361009 | 1-557 | Nucleoprotein | |||
Sequence: MSGASEVPSFRWTQSLRRGLSHFTTSAKGDVLRDAKSLVDGLDFNQVSQVQRVMRKDKRSDDDLSKLRDLNRSVDSLMVMKNKQNNVSLKIGSLSKDELMDLATDLEKLKRKINLGDRQGPGVYQGNLTSAQLEKRSEILKSLGFQPRANQNGVVKVWDIKNPKLLINQFGSIPALTIACMSVQGAEQMNDVVQGLTSLGLLYTVKYPNLDDLDKLSKDHPCLEFITKEESANNISGYNLSLSAAVKAGACLVDGGNMLETILVKPDNFQDIVKSLLVVKRQEKMFVNEKPGLRNPYENILYKLCLSGEGWPYIGSRSQIVGRAWENTTVDLSKEVVYGPSAPVKNGGNMRLSPLSDTQEAVIKEAIGKLDMDETIWIDIEGPPNDPVELAIYQPSTGNYIHCFRVPHDEKGFKNGSKYSHGILLRDIENARSGLLSRILMRLPQKVVFTCQGSDDIQKLLQMNGRPDIATIDMSFSSEQARFFEGVVWEKFGHLCTRHNGVVLSRKKKGGNSGEPHCALLDCIIFQAAFEGQVTGQIPKPLLPNSLIFKDEPRVAM |
Interaction
Subunit
Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity (PubMed:22532683).
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 54-235 | Binding site for the cap structure m7GTP | ||||
Sequence: MRKDKRSDDDLSKLRDLNRSVDSLMVMKNKQNNVSLKIGSLSKDELMDLATDLEKLKRKINLGDRQGPGVYQGNLTSAQLEKRSEILKSLGFQPRANQNGVVKVWDIKNPKLLINQFGSIPALTIACMSVQGAEQMNDVVQGLTSLGLLYTVKYPNLDDLDKLSKDHPCLEFITKEESANNI |
Domain
The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.
Sequence similarities
Belongs to the arenaviridae nucleocapsid protein family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length557
- Mass (Da)61,870
- Last updated2003-03-01 v1
- ChecksumDA1A83B1BD7DC0C0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF485259 EMBL· GenBank· DDBJ | AAN09941.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
AF512830 EMBL· GenBank· DDBJ | AAN32960.1 EMBL· GenBank· DDBJ | Genomic RNA |