Q8BDE6 · NCAP_LATVB

Function

function

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.

Features

Showing features for binding site, site.

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TypeIDPosition(s)Description
Binding site379Mn2+ (UniProtKB | ChEBI)
Binding site381Mn2+ (UniProtKB | ChEBI)
Binding site389Zn2+ (UniProtKB | ChEBI)
Site456Important for exonuclease activity
Binding site496Zn2+ (UniProtKB | ChEBI)
Binding site499Zn2+ (UniProtKB | ChEBI)
Binding site518Zn2+ (UniProtKB | ChEBI)
Binding site522Mn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell cytoplasm
Cellular Componentribonucleoprotein complex
Cellular Componentviral nucleocapsid
Molecular Functionhydrolase activity
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Biological Processnegative stranded viral RNA replication
Biological ProcessRNA-templated viral transcription
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity
Biological Processvirus-mediated perturbation of host defense response

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoprotein
  • EC number
  • Alternative names
    • Nucleocapsid protein
    • Protein N

Gene names

    • Name
      N

Organism names

Accessions

  • Primary accession
    Q8BDE6
  • Secondary accessions
    • Q8BD29

Proteomes

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant214
Natural variant279

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003610091-557Nucleoprotein

Interaction

Subunit

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity (PubMed:22532683).

Structure

3D structure databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region54-235Binding site for the cap structure m7GTP

Domain

The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.

Sequence similarities

Belongs to the arenaviridae nucleocapsid protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    557
  • Mass (Da)
    61,870
  • Last updated
    2003-03-01 v1
  • Checksum
    DA1A83B1BD7DC0C0
MSGASEVPSFRWTQSLRRGLSHFTTSAKGDVLRDAKSLVDGLDFNQVSQVQRVMRKDKRSDDDLSKLRDLNRSVDSLMVMKNKQNNVSLKIGSLSKDELMDLATDLEKLKRKINLGDRQGPGVYQGNLTSAQLEKRSEILKSLGFQPRANQNGVVKVWDIKNPKLLINQFGSIPALTIACMSVQGAEQMNDVVQGLTSLGLLYTVKYPNLDDLDKLSKDHPCLEFITKEESANNISGYNLSLSAAVKAGACLVDGGNMLETILVKPDNFQDIVKSLLVVKRQEKMFVNEKPGLRNPYENILYKLCLSGEGWPYIGSRSQIVGRAWENTTVDLSKEVVYGPSAPVKNGGNMRLSPLSDTQEAVIKEAIGKLDMDETIWIDIEGPPNDPVELAIYQPSTGNYIHCFRVPHDEKGFKNGSKYSHGILLRDIENARSGLLSRILMRLPQKVVFTCQGSDDIQKLLQMNGRPDIATIDMSFSSEQARFFEGVVWEKFGHLCTRHNGVVLSRKKKGGNSGEPHCALLDCIIFQAAFEGQVTGQIPKPLLPNSLIFKDEPRVAM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF485259
EMBL· GenBank· DDBJ
AAN09941.1
EMBL· GenBank· DDBJ
Genomic RNA
AF512830
EMBL· GenBank· DDBJ
AAN32960.1
EMBL· GenBank· DDBJ
Genomic RNA

Genome annotation databases

Similar Proteins

Disclaimer

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